MRI: Acquisition of a Pulsed Electron Paramagnetic Resonance (EPR) Spectrometer

该奖项由主要研究仪器计划（MRI）和化学研究仪器计划支持。来自牛津（俄亥俄州）迈阿密大学的加里·洛里根教授和他的同事迈克尔·克劳德、大卫·蒂尔尼、卡罗尔·达布尼-史密斯和理查德·佩奇正在获得一台X/Q波段脉冲电子顺磁共振（EPR）光谱仪。该仪器可用于生物学和环境研究等研究具有未成对电子的分子的各种领域的研究。一般来说，EPR光谱仪产生分子和固态材料的几何和电子结构的详细信息。它也可以用来获得有关未成对电子寿命的信息，因为它们是参与许多化学转化的短寿命，高反应性物种。研究生和本科生（包括来自NSF本科生研究经验（REU）计划的学生）在研究人员的实验室接受培训，以便在学术界和工业界的职业生涯中具有竞争力。附近的中央州立大学（一所历史上的黑人学院或大学（HBCU））的学生也可以使用这种仪器。这台EPR光谱仪加强了各级的研究和教育。它有助于研究人员探测KCNE 1/Q1通道复合物的结构;阐明金属-B-内酰胺酶的不同底物和抑制剂的结合机制，并研究高自旋钴（II）磁性材料和酶的电子状态。该仪器还用于确定叶绿体膜运输机制和研究参与肌肉收缩和信号转导的蛋白质。该光谱仪用于探测CHIP/Hsp 70蛋白复合物的蛋白质-蛋白质相互作用，并研究同源/异源寡聚蛋白的结构/结合。 最后，EPR光谱仪作为其他国家机构和附近大学的区域资源。

This award is supported by the Major Research Instrumentation Program (MRI) and the Chemistry Research Instrumentation Program. Professor Gary Lorigan from Miami University at Oxford (Ohio) and colleagues Michael Crowder, David Tierney, Carole Dabney-Smith and Richard Page are acquiring an X-/Q-band pulsed electron paramagnetic resonance (EPR) spectrometer. This instrument allows research in a variety of fields that study molecules with unpaired electrons such as biology and environmental studies. In general, an EPR spectrometer yields detailed information on the geometric and electronic structure of molecular and solid state materials. It may also be used to obtain information about the lifetimes of the unpaired electrons as they are short-lived, highly reactive species involved in many chemical transformations. Graduate and undergraduate students (including those from in an NSF Research Experiences for Undergraduates (REU) program) are trained in the researchers' laboratories to be competitive in careers in academia and industry. Students from the nearby Central State University (an Historically Black College or University (HBCU)) also have access to this instrumentation. This EPR spectrometer enhances research and education at all levels. It aids researchers in probing the structure of the KCNE1/Q1 channel complex; elucidating the binding mechanisms of different substrates and inhibitors for metallo-B-lactamases, and studying the electronic state of high-spin cobalt(II) magnetic materials and enzymes. The instrumentation is also used in determining the chloroplast membrane transport mechanisms and studying the proteins involved in muscle contraction and signal transduction. The spectrometer is employed in probing the protein-protein interactions of the CHIP/Hsp70 protein complex and investigating the structure/binding of homo/hetero-oligomeric proteins. Finally, the EPR spectrometer serves as a regional resource for other state institutions and nearby universities.