MRI: Acquisition of a Pulsed EPR Spectrometer for Miami University

牛津迈阿密大学化学和生物化学系获得了重大研究仪器计划(MRI)颁发的这一奖项，将获得一台脉冲EPR光谱仪。脉冲EPR光谱仪将用于几个研究项目，包括1)膜蛋白的结构表征，2)金属内酰胺酶的结构测定，3)ATP和/或DNA与粘附素复合体的结构测定和结合，4)各种HMGA1/DNA/蛋白质复合体的结构性质，以及5)几种独特的纳米结构人工酶/蛋白质的表征。这将是第一个放置在俄亥俄州学术机构的脉冲EPR光谱仪，访问范围将扩大到俄亥俄州的其他六个学术机构。电子顺磁共振(EPR)光谱仪可以获得关于分子的分子和电子结构的信息。它还可以用来获取有关自由基寿命的信息，自由基是一种寿命较短的物种，通常对启动肿瘤生长和/或各种化学反应至关重要。脉冲电子顺磁共振(EPR)方法，如脉冲Endor，已被成功地用于研究生物样品中顺磁中心的局部结构和动力学。不同的脉冲EPR方法可以区分不同的EPR谱，并有助于解开复杂的EPR谱，这些谱由重叠的共振线组成，通常在无序的蛋白质样品中发现。

With this award from the Major Research Instrumentation program (MRI), the Department of Chemistry and Biochemistry at Miami University at Oxford will acquire a pulsed EPR spectrometer. The pulsed EPR spectrometer will be utilized in several research projects including 1) structural characterization of membrane proteins, 2) structural determination of Metallo-â-lactamases, 3) structural determination and binding of ATP and/or DNA to the Cohesin Complex, 4) structural properties of various HMGA1/DNA/protein complexes, and 5) characterization of several unique nanostructured artificial enzymes/proteins. This would be the first pulsed EPR spectrometer placed at an academic institution in the state of Ohio and access would be expanded to include six other academic institutions in the state of Ohio.An electron paramagnetic resonance (EPR) spectrometer yields information on the molecular and electronic structure of molecules. It may also be used to obtain information about the lifetimes of free radicals, short-lived species that are often essential for the initiation of tumor growth and/or a variety of chemical reactions. Pulsed electron paramagnetic resonance (EPR) methods such as pulsed ENDOR, have been successfully utilized to investigate the local structure and dynamics of paramagnetic centers in biological samples. Different pulsed EPR methods allow different contributions to the EPR spectra to be distinguished and can help unravel complicated EPR spectra consisting of overlapping resonance lines, often found in disordered protein samples.