Comparative analysis of luteotropic and luteolytic factors in CLs of domestic cats and Eurasian lynx

家猫与欧亚山猫 CL 中促黄体素和黄体分解因子的比较分析

• 批准号: 230375173
• 负责人: Professorin Dr. Katarina Jewgenow
• 金额: --
• 依托单位: Leibniz-Institut für Zoo- und Wildtierforschung (IZW)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/230375173?language=en
• 关键词: Comparative; analysis; luteotropic; luteolytic; factors

Assisted reproductive techniques offer a great potential for conservation of endangered species. The Iberian lynx is the only felid species which is listed a critically endangered on IUCN Red List. In 2000 a scientific captive breeding program was established to accompany the in-situ-conservation of Iberian lynx. The success of the captive breeding program depends on knowledge on reproduction and reproduction management. The application of artificial insemination within the captive breeding program is compromised by the phenomenon of persistent corpora lutea (CLs) in lynx which hamper ovulation induction outside breeding season. Aim of project is the comparative analysis of luteotropic and luteolytic factors in CLs of domestic cats and Eurasian lynx to investigate the functional life cycles of CLs in the two felid species. Factors involved in the organization (after ovulation), function (comparison between pregnancy and pseudopregnancy) and regression will be examined on structural (histology, immunohistology), endocrine (hormones and hormone receptors), molecular (expression analysis) and biochemical levels (proteome analysis). In lynx, ovaries will be obtained during the whole year with emphasis on breeding season. We suggest that persistent CLs in lynx are a result of the decoupling of functional and structural luteolysis.

辅助生殖技术为保护濒危物种提供了巨大的潜力。伊比利亚猞猁是世界上唯一被世界自然保护联盟列为极度濒危物种的猫科动物。2000年建立了一个科学的圈养繁殖计划，以配合伊比利亚猞猁的就地保护。圈养繁殖计划的成功取决于繁殖和繁殖管理方面的知识。人工授精在圈养繁殖计划中的应用受到猞猁体内持续黄体（CLs）现象的影响，这种现象阻碍了繁殖季节以外的排卵诱导。本项目旨在比较分析家猫和欧亚猞猁CLs中促黄体和溶黄体因子，探讨两种猫科动物CLs的功能生命周期。将从结构（组织学、免疫组织学）、内分泌（激素和激素受体）、分子（表达分析）和生化水平（蛋白质组学分析）等方面检查组织（排卵后）、功能（妊娠与假妊娠比较）和回归的相关因素。猞猁的卵巢采集在全年进行，主要集中在繁殖季节。我们认为，猞猁的持续CLs是功能性和结构性黄体溶解解耦的结果。