CAREER: ENGINEER A FUNCTIONAL 3-D VASCULAR NICHE TO SUPPORT NEURAL STEM CELL SELF-RENEWAL

职业:设计功能性 3D 血管生态位以支持神经干细胞自我更新

基本信息

  • 批准号:
    1737130
  • 负责人:
  • 金额:
    $ 25.95万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-03-01 至 2020-03-31
  • 项目状态:
    已结题

项目摘要

1350240DaiAdult neural stem cells (NSCs) have great potential for treating damage or disease of the nervous system. However, these cells are rare and difficult to maintain in culture because they quickly lose their multi-potency after being removed from their native environment. In vivo, NSCs reside adjacent to a vascular niche, suggesting that it may be possible to maintain these cells ex vivo if critical functional features of the vascular niche are recreated in the culture environment.The hypothesis of the proposed studies is that formation of a perfused, functional vasculature within 3-D extracellular matrix promotes self-renewal of the surrounding NSCs. To test this hypothesis, the following specific aims will be pursued: 1) Create a neurovascular unit that contains perfused vasculature and NSCs within 3-D matrix using a novel cell printing technology. 2) Establish the maturation process and functionalities of the synthesized structures with regard to matrix properties and culture parameters. 3) Examine the NSC behavior and cell fate using the engineered neurovascular unit; and determine critical factors that influence NSC fate and its relationship to the functional status of the vasculature.Intellectual Merit: The proposed research will build a 3-D perfused vascular niche to study NSCs in a physiologically relevant experimental system, in which individual components and parameters can be easily manipulated. Using this system, the proposed research will define critical factors to achieve functionality of the vasculature and will elucidate underlying mechanisms of NSC self-renewal in the dynamically perfused system. Since vasculature is a key element of many adult stem cell niches, this research will have broader ramifications in studying other stem cell fields. Overall, this work will identify design principles and operating parameters for the printed structures to support the growth of the surrounding tissues in vitro, and thereby lay the foundation to fabricate tissues of appropriate thickness for biomedical applications in the future.Broader Impacts: The proposed research has broader scientific impact in generating fundamental knowledge related to 3-D vascular tissue formation and its role in regulating NSC fate. Therefore, the proposed studies are expected to help develop methods for the ex vivo expansion of patient-specific NSCs to treat spinal cord injury and neurodegenerative diseases, which will benefit patients and the society at large. Research will be integrated with educational and outreach activities targeting K-12 education and underrepresented groups. Activities demonstrating how engineers can solve societal challenges will provide incentives to students to pursue careers in science and engineering.
1350240 DaiAdult neural stem cells(NSC)具有治疗神经系统损伤或疾病的巨大潜力。 然而,这些细胞是罕见的,难以在培养中维持,因为它们在从其天然环境中移除后很快失去其多能性。 在体内,神经干细胞居住在邻近的血管龛,这表明它可能是可能的,以维持这些细胞离体,如果血管龛的关键功能功能的特点是在培养environment.The假设提出的研究是,形成一个灌注,功能性血管内的3-D细胞外基质促进自我更新周围的神经干细胞。 为了检验这一假设,将追求以下具体目标:1)使用新的细胞打印技术在3-D基质内创建包含灌注的脉管系统和NSC的神经血管单元。 2)根据基质特性和培养参数,建立合成结构的成熟过程和功能。 3)检查神经干细胞的行为和细胞的命运,使用工程神经血管单位,并确定影响神经干细胞的命运和它的关系的功能状态的vascularis.Intellectual优点:拟议的研究将建立一个3-D灌注血管生态位研究神经干细胞在生理相关的实验系统,其中个别组件和参数可以很容易地操纵的关键因素。 使用这个系统,拟议的研究将定义关键因素,以实现功能的血管,并将阐明潜在的机制,神经干细胞自我更新的动态灌注系统。由于脉管系统是许多成体干细胞龛的关键因素,这项研究将在研究其他干细胞领域产生更广泛的影响。 总体而言,这项工作将确定打印结构的设计原则和操作参数,以支持体外周围组织的生长,从而为未来生物医学应用中制造适当厚度的组织奠定基础。拟议的研究在产生与3-D血管组织形成及其在调节NSC命运中的作用相关的基础知识方面具有更广泛的科学影响。 因此,拟议的研究有望帮助开发用于患者特异性NSC的体外扩增的方法,以治疗脊髓损伤和神经退行性疾病,这将使患者和整个社会受益。 研究将与针对K-12教育和代表性不足群体的教育和外联活动相结合。 展示工程师如何解决社会挑战的活动将激励学生追求科学和工程事业。

项目成果

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Guohao Dai其他文献

Enabling Fast 2-bit LLM on GPUs: Memory Alignment and Asynchronous Dequantization
在 GPU 上启用快速 2 位 LLM:内存对齐和异步反量化
  • DOI:
    10.48550/arxiv.2308.13137
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Jinhao Li;Shiyao Li;Jiaming Xu;Shan Huang;Yaoxiu Lian;Jun Liu;Yu Wang;Guohao Dai
  • 通讯作者:
    Guohao Dai
Comprehensive models and analytical solutions for transient heat transfer in PHC energy pile
PHC 能源桩瞬态传热的综合模型与解析解
  • DOI:
    10.1016/j.compgeo.2024.106713
  • 发表时间:
    2024-12-01
  • 期刊:
  • 影响因子:
    6.200
  • 作者:
    Guohao Dai;Gangqiang Kong;Huafeng Deng;Qing Yang
  • 通讯作者:
    Qing Yang
Exploiting Online Locality and Reduction Parallelism for Sampled Dense Matrix Multiplication on GPUs
利用在线局部性和约简并行性进行 GPU 上的采样密集矩阵乘法
Analytical solution of heat transfer for energy soldier piles considering convection at the ground surface and internal wall of underground space
考虑地下空间地表和内壁对流的能源士兵桩传热分析解
  • DOI:
    10.1016/j.compgeo.2025.107150
  • 发表时间:
    2025-05-01
  • 期刊:
  • 影响因子:
    6.200
  • 作者:
    Guohao Dai;Gangqiang Kong;Qing Yang
  • 通讯作者:
    Qing Yang
Memory-Bound Proof-of-Work Acceleration for Blockchain Applications
区块链应用程序的内存限制工作证明加速
  • DOI:
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kun Wu;Guohao Dai;Xing Hu;Shuangchen Li;Xinfeng Xie;Yu Wang;Yuan Xie
  • 通讯作者:
    Yuan Xie

Guohao Dai的其他文献

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{{ truncateString('Guohao Dai', 18)}}的其他基金

2020 Biomedical Engineering Society (BMES) - Cellular and Molecular Bioengineering (CMBE) Conference; Rio Grande, Puerto Rico; January 2-6, 2020
2020年生物医学工程学会(BMES)-细胞与分子生物工程(CMBE)会议;
  • 批准号:
    1933397
  • 财政年份:
    2019
  • 资助金额:
    $ 25.95万
  • 项目类别:
    Standard Grant
CAREER: ENGINEER A FUNCTIONAL 3-D VASCULAR NICHE TO SUPPORT NEURAL STEM CELL SELF-RENEWAL
职业:设计功能性 3D 血管生态位以支持神经干细胞自我更新
  • 批准号:
    1350240
  • 财政年份:
    2014
  • 资助金额:
    $ 25.95万
  • 项目类别:
    Standard Grant
Integrated Platform to Construct and Image 3-D Perfused Vascular Network Within Thick Matrix
用于在厚基质内构建和成像 3D 灌注血管网络的集成平台
  • 批准号:
    1263455
  • 财政年份:
    2013
  • 资助金额:
    $ 25.95万
  • 项目类别:
    Continuing Grant

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MFB:移码刺激器的RNA修饰:通过计算突变预测和功能实验来设计基因表达的细胞平台
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    2330628
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    2024
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Structural and functional studies on African horse sickness virus (AFHV) to engineer a protein-based vaccine prototype
对非洲马瘟病毒 (AFHV) 进行结构和功能研究,以设计基于蛋白质的疫苗原型
  • 批准号:
    2747609
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    2022
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    Studentship
Collaborative Research: Engineer a functional 3D vascularized islet organoid from pluripotent stem cells
合作研究:利用多能干细胞设计功能性 3D 血管化胰岛类器官
  • 批准号:
    1842675
  • 财政年份:
    2018
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Collaborative Research: Engineer a functional 3D vascularized islet organoid from pluripotent stem cells
合作研究:利用多能干细胞设计功能性 3D 血管化胰岛类器官
  • 批准号:
    1706742
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Collaborative Research: Engineer a functional 3D vascularized islet organoid from pluripotent stem cells
合作研究:利用多能干细胞设计功能性 3D 血管化胰岛类器官
  • 批准号:
    1706674
  • 财政年份:
    2017
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    $ 25.95万
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    Standard Grant
Collaborative Research: Engineer a functional 3D vascularized islet organoid from pluripotent stem cells
合作研究:利用多能干细胞设计功能性 3D 血管化胰岛类器官
  • 批准号:
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CAREER: ENGINEER A FUNCTIONAL 3-D VASCULAR NICHE TO SUPPORT NEURAL STEM CELL SELF-RENEWAL
职业:设计功能性 3D 血管生态位以支持神经干细胞自我更新
  • 批准号:
    1350240
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    $ 25.95万
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    Standard Grant
Exploiting the self-assembly of hydrophobin proteins to engineer functional nanostructuring surfaces
利用疏水蛋白的自组装来设计功能性纳米结构表面
  • 批准号:
    DP1093949
  • 财政年份:
    2010
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    $ 25.95万
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    Discovery Projects
Using drugs, machines, and genes to engineer functional muscle
使用药物、机器和基因来设计功能性肌肉
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Identification of tumour-antigen specific T cell receptors for non-viral CRISPR/Cas9 mediated orthotopic- vs. retro-viral transduction to engineer high-functional T cell responses against Ewing’s sarcoma
鉴定非病毒 CRISPR/Cas9 介导的原位病毒转导与逆转录病毒转导的肿瘤抗原特异性 T 细胞受体,以设计针对尤文氏肉瘤的高功能 T 细胞反应
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    501829858
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    $ 25.95万
  • 项目类别:
    Heisenberg Grants
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