Collaborative Research: EAGER: The early evolution of lamp shells and relatives (brachiopods) using an integrated approach combining genomics and fossils

合作研究：EAGER：使用基因组学和化石相结合的综合方法研究灯壳及其亲戚（腕足动物）的早期进化

• 批准号: 1747704
• 负责人: Sandra Carlson
• 金额: $ 7.95万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1747704&HistoricalAwards=false
• 关键词: Collaborative; Research; EAGER; early; evolution

Lamp shells and relatives (brachiopods) are marine animals that are one of the world's oldest forms of multicellular organisms. Although no longer numerous today, brachiopod species were a dominant component of marine ecosystems 540-250 million years ago. Because of their mineralized shells, brachiopods have a high chance of being preserved as fossils, and have one of the most complete fossil records of any animal group. This project aims to solve a long-standing debate regarding how different brachiopod groups are related to each other and to other animals. Living brachiopods from North America, Croatia, Japan, New Zealand, and several other localities around the world will be sampled for genetic material that will be used to reconstruct evolutionary relationships among brachiopods. These genetic data will then be combined with data from their rich fossil record to test whether rates of evolution were higher during the Cambrian period than during more recent time periods. Several workshops will be organized to train a new generation of early career scientists in a diversity of genomic and paleontological methods. This project will also develop new instructional videos and accompanying lesson plans designed to teach high school science students how to build earth science content from a biological foundation. Brachiopods are amongst the first mineralized metazoans to appear in the fossil record during the Cambrian, yet agreement about the phylogenetic relationships among these early forms is still unknown. While the brachiopod fossil record will ultimately be key to determining character homology and polarity during the evolution of the distinctive brachiopod body plan, correctly reading this record has been difficult due to disagreement about relationships among the living clades. This project will use an integrated approach to understand the phylogeny of brachiopods and macroevolutionary patterns of early animal evolution. First, a widespread fieldwork campaign will collect brachiopod specimens suitable for high-quality RNA extraction and transcriptome sequencing. These data will form the basis for the heart of this study: the first phylogenomic study of brachiopods, which will result in a robust phylogenetic framework for the modern clades. Second, a comprehensive morphological database for living and fossil brachiopods will be constructed and calibrated using a molecular clock. These molecular and morphological data sources will be combined to test hypotheses of morphological and molecular evolution during the rapid diversification of form during the Cambrian radiation.

贝壳类动物及其近亲（腕足类）是海洋动物，是世界上最古老的多细胞生物之一。虽然腕足动物的数量在今天已经不多了，但它们在5.4亿至2.5亿年前是海洋生态系统的主要组成部分。 由于它们的矿化外壳，腕足动物有很高的机会被保存为化石，并且拥有任何动物群中最完整的化石记录之一。该项目旨在解决一个长期存在的争论，即不同的腕足动物群体之间以及与其他动物之间的关系。来自北美、克罗地亚、日本、新西兰和世界其他几个地方的活腕足动物将被取样，以获取遗传物质，用于重建腕足动物之间的进化关系。这些遗传数据将与丰富的化石记录数据相结合，以测试寒武纪时期的进化速率是否高于最近的时期。将组织几个讲习班，以培训新一代的早期职业科学家在基因组学和古生物学方法的多样性。该项目还将开发新的教学视频和配套的课程计划，旨在教高中理科学生如何从生物学基础上构建地球科学内容。腕足动物是寒武纪化石记录中最早出现的矿化后生动物之一，但关于这些早期形式之间的系统发育关系的协议仍然是未知的。虽然腕足动物化石记录最终将是确定特征同源性和极性的关键，在独特的腕足动物身体计划的演变过程中，正确阅读这一记录一直是困难的，由于生活分支之间的关系存在分歧。这个项目将使用一个综合的方法来理解腕足动物的进化和早期动物进化的宏观进化模式。首先，广泛的实地工作将收集适合高质量RNA提取和转录组测序的腕足动物标本。这些数据将构成本研究核心的基础：第一个腕足动物的基因组学研究，这将为现代分支提供一个强大的系统发育框架。第二，将建立一个全面的腕足动物活体和化石形态数据库，并使用分子钟进行校准。这些分子和形态学数据源将结合起来，以测试形态和分子进化的假设，在寒武纪辐射期间的形式迅速多样化。

项目成果

Brachiopod phylogenomics: implications for the evolutionary history of biomineralization and the Cambrian explosion

腕足动物系统发育组学：对生物矿化和寒武纪大爆发进化史的影响

• 发表时间: 2019
• 期刊: PaleoBios
• 作者: Butler, A. D.;Eitel, M.;Wörheide, G.;Carlson, S.J.;Sperling, E.A.
• 通讯作者: Sperling, E.A.

Testing species assignments in extant terebratulide brachiopods: A three-dimensional geometric morphometric analysis of long-looped brachidia

测试现存的terebratulide腕足动物的物种分配：长环腕足动物的三维几何形态测量分析

• DOI: 10.1371/journal.pone.0225528
• 发表时间: 2019
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Lopez Carranza, N.;Carlson, S. J.
• 通讯作者: Carlson, S. J.

Terebratulina: is 81 species too many?

Terebratulina：81个物种太多了吗？

• 发表时间: 2017
• 期刊: Abstracts with Programs,
• 作者: Carlson, S. J.;Bapst, D. W.;Robinson, J. H.;Rudman, E. A.;Lopez-Carranza, N.
• 通讯作者: Lopez-Carranza, N.

Phylogenomic analysis of Brachiopoda: revealing the evolutionary history of biomineralization with an integrated palaeontological and molecular approach

腕足动物的系统发育分析：通过综合古生物学和分子方法揭示生物矿化的进化史

• 发表时间: 2018
• 期刊: Permophiles
• 作者: Butler, A. D.;Eitel, M.;Wörheide, G.;Carlson, S. J.;Sperling, E. A.
• 通讯作者: Sperling, E. A.

The role of ontogenetic transformations in the loop in the classification and phylogeny of Terebratellidina (Brachiopoda)

环中个体发生转化在腕足动物分类和系统发育中的作用

• 发表时间: 2019
• 期刊: PaleoBios
• 作者: Carlson, S.J.;López Carranza, N.;Butler, A.D.;Bapst, D.W.;Sperling, E.A.
• 通讯作者: Sperling, E.A.