SG: Collaborative Research: Measuring intra-locus conflict across the genome in a dioecious plant

SG：合作研究：测量雌雄异株植物基因组中的位点内冲突

• 批准号: 1753629
• 负责人: Lynda Delph
• 金额: $ 9.81万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1753629&HistoricalAwards=false
• 关键词: SG; Collaborative; Research; Measuring; intra

Understanding natural selection is an important scientific objective that furthers our understanding of the natural world and impacts human health and welfare. Modern agriculture is based on the adoption of selection as a method to improve crops. Furthermore, dealing with emerging diseases and antibiotic resistance is helped by understanding how natural selection operates. This project examines the fact that the strength and direction of natural selection routinely differs between males and females of a species. The consequence of this selection depends on whether the same genes are expressed in males and females. The research team will explore selection on males and females using field studies, genomic analyses, and statistical modeling using Silene latifolia, an herbaceous flowering plant. This plant is an ideal model for this research given extensive prior work demonstrating important phenotypic and genetic differences between males and females. Workshops to the scientific community will be presented on how to write scientific articles and the genomic techniques used in this study. Outreach to the general public will include a project involving high-school students in Kansas in genetic research, and a demonstration on flowers at a community-level science festival in Indiana.The research team will collect phenotypic and fitness measurements on all individuals within a S. latifolia population from Virginia, and then interrogate these individuals, and their progeny, at Single Nucleotide Polymorphisms (SNPs) across the genome. The combination of phenotypic, genotypic, and fitness data will allow the researchers to determine if selection is evident at the scale of individual loci and if it differs between males and females. This species has heteromorphic sex chromosomes (males are XY), and a genome-wide characterization of selection will enable a test of the prediction that selection should differ between autosomes and sex chromosomes. While there is a great body of work measuring natural selection in plant populations, this experiment will provide unprecedented detail on a typically hidden component of selection, the differential success of male plants in siring. Finally, the project will allow both undergraduate and graduate students to engage in and learn field techniques, laboratory methodology, and bioinformatics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

了解自然选择是一个重要的科学目标，它可以加深我们对自然世界的了解，并影响人类的健康和福祉。现代农业的基础是采用选择作为改进作物的方法。此外，了解自然选择如何运作有助于应对新出现的疾病和抗生素耐药性。这个项目研究了自然选择的强度和方向在一个物种的雄性和雌性之间通常不同的事实。这种选择的结果取决于相同的基因是否在男性和女性中表达。该研究小组将使用田间研究，基因组分析和统计建模来探索对雄性和雌性的选择，使用的是一种草本开花植物Silene latifolia。这种植物是这项研究的理想模型，因为大量的前期工作证明了雄性和雌性之间重要的表型和遗传差异。研讨会将介绍科学界如何撰写科学文章和本研究中使用的基因组技术。对公众的宣传将包括一个涉及堪萨斯高中生遗传研究的项目，以及在印第安纳州社区科学节上展示花卉。来自弗吉尼亚州的阔叶树种群，然后询问这些个体及其后代，在整个基因组中的单核苷酸多态性（SNP）。表型、基因型和适应性数据的结合将使研究人员能够确定选择在单个基因座的尺度上是否明显，以及男性和女性之间是否存在差异。该物种具有异形性染色体（雄性为XY），全基因组范围内的选择特征将能够测试常染色体和性染色体之间的选择应该不同的预测。虽然有大量的工作测量植物种群中的自然选择，这个实验将提供前所未有的细节选择的一个典型的隐藏的组成部分，雄性植物在繁殖中的差异成功。最后，该项目将允许本科生和研究生参与和学习现场技术，实验室方法和生物信息学。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。