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Targeting of salicylic acid-activated NPR1 by a bacterial type III effector

III 型细菌效应子靶向水杨酸激活的 NPR1

基本信息

批准号：
1758994
负责人：
Zhengqing Fu
金额：
$ 48.76万
依托单位：
University of South Carolina at Columbia
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2021-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1758994&HistoricalAwards=false
关键词：
Targeting salicylic acid activated NPR1

项目摘要

Plant diseases caused by pathogens pose a major threat to food security worldwide. A deeper understanding of how plant pathogens cause diseases lays foundations for developing effective strategies to keep plant diseases under control for sustainable agriculture. Many plant pathogens including fungi, bacteria, nematodes, bacteria, and oomycetes rely on effectors that they deliver into plant cells to suppress plant defenses and establish infection. The plant hormone salicylic acid plays an essential role in plant defense against pathogen infection. The investigators discovered that a bacterial type III effector targets the master regulator of salicylic acid-mediated plant defense for degradation, causing disease. This project aims to elucidate the molecular mechanism and biological consequences of the bacterial effector-mediated degradation of the master regulator, and identify a mutant form of the regulator, which cannot be degraded by the effector. This non-degradable regulator could potentially be used to generate disease-resistant plants. Disease-resistant crops are critical to feed a global world population on a decreasing amount of arable land, and to reduce the amount of environmentally harmful pesticides. Two graduate and four undergraduate students will be supported through this project each year. Funding will also be used to train middle school teachers and students in South Carolina to detect citrus greening disease before citrus trees show obvious symptoms in SCienceLab. By connecting hands-on cutting-edge experimentation with urgent real life plant pathology problems, this project will inspire students and make them aware of the importance of plant science. This project focuses on the targeting of SA-activated NPR1 by the Psudomonas syringae type III effector AvrPtoB. Though it has been known for many years that NPR1 plays an essential role in both local and systemic plant defense, it has not been reported that a pathogen effector targets NPR1. SA facilitates the reduction of plant cytosolic NPR1 oligomers into monomers, which enter the nucleus and function as transcriptional coactivators of plant defense genes. This project showed that SA promotes the interaction between AvrPtoB and NPR1, suggesting that AvrPtoB only interacts with the active form of NPR1. This project demonstrated that AvrPtoB targets NPR1 for degradation, dependent on AvrPtoB's E3 ligase activity. In addition, this project found that the master regulator of SA signaling, NPR1, plays an important role in MTI (MAMP-triggered immunity). This proposal seeks to 1) investigate how SA promotes the interaction between NPR1 and AvrPtoB by determining if AvrPtoB only targets monomeric NPR1 protein, 2) determine the molecular mechanism of AvrPtoB-mediated poly-ubiquitination and degradation of NPR1, 3) show how NPR1 contributes to MTI, and 4) determine how AvrPtoB targets SA-activated NPR1 to disrupt NPR1-dependent MTI to subvert plant innate immunity. These studies will provide fresh insights into effector biology and increase our understanding of MTI and the molecular and biological functions of NPR1 and SA in plant defense. The work will involve training of two postdoctoral fellows to perform research and mentoring of undergraduate students from under-represented groups to empower them to pursue careers in scientific disciplines.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

病原体引起的植物病害对全世界粮食安全构成重大威胁。更深入地了解植物病原体如何引起疾病，为制定有效的战略奠定了基础，以控制植物疾病，实现可持续农业。许多植物病原体，包括真菌、细菌、线虫、细菌和卵菌，依赖于它们递送到植物细胞中的效应物来抑制植物防御并建立感染。植物激素水杨酸在植物防御病原体感染中起重要作用。研究人员发现，一种细菌III型效应子靶向水杨酸介导的植物防御的主要调节因子，使其降解，引起疾病。本项目旨在阐明细菌效应子介导的主调节子降解的分子机制和生物学后果，并鉴定不能被效应子降解的调节子的突变形式。这种不可降解的调节剂有可能用于产生抗病植物。抗病作物对于在日益减少的可耕地上养活全球人口以及减少对环境有害的农药数量至关重要。每年将通过该项目资助两名研究生和四名本科生。资金还将用于培训南卡罗来纳州的中学教师和学生，在柑橘树在SCienceLab中表现出明显症状之前检测柑橘绿化病。通过将动手尖端实验与紧迫的真实的生命植物病理学问题联系起来，该项目将激励学生并使他们意识到植物科学的重要性。该项目的重点是靶向SA激活的NPR1的Psuspiciumingae III型效应AvrPtoB。尽管多年来人们已经知道NPR 1在局部和系统性植物防御中发挥着重要作用，但尚未有病原体效应子靶向NPR 1的报道。SA促进植物胞质NPR 1寡聚体还原成单体，单体进入细胞核并作为植物防御基因的转录共激活因子发挥作用。该项目表明SA促进AvrPtoB和NPR1之间的相互作用，表明AvrPtoB仅与NPR1的活性形式相互作用。该项目表明，AvrPtoB靶向NPR 1降解，这取决于AvrPtoB的E3连接酶活性。此外，该项目发现SA信号传导的主调节因子NPR 1在MTI（MAMP触发免疫）中起重要作用。该提案旨在1）通过确定AvrPtoB是否仅靶向单体NPR 1蛋白来研究SA如何促进NPR 1和AvrPtoB之间的相互作用，2）确定AvrPtoB介导的NPR 1的多聚泛素化和降解的分子机制，3）显示NPR 1如何有助于MTI，以及4）确定AvrPtoB如何靶向SA激活的NPR 1以破坏NPR 1依赖的MTI从而破坏植物先天免疫。这些研究将为效应生物学提供新的见解，并增加我们对MTI以及NPR1和SA在植物防御中的分子和生物学功能的理解。这项工作将包括培训两名博士后研究员进行研究，并指导来自代表性不足群体的本科生，使他们能够追求科学学科的职业生涯。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling, NPR1, to Subvert Plant Immunity

DOI：
10.1016/j.chom.2017.10.019
发表时间：
2017-12-13
期刊：
CELL HOST & MICROBE
影响因子：
30.3
作者：
Chen, Huan;Chen, Jian;Fu, Zheng Qing
通讯作者：
Fu, Zheng Qing

Salicylic acid-mediated plant defense: Recent developments, missing links, and future outlook

DOI：
10.1007/s11515-017-1460-4
发表时间：
2017-09
期刊：
Frontiers in Biology
影响因子：
0
作者：
I. Palmer;Zhenhua Shang*;Z. Fu
通讯作者：
I. Palmer;Zhenhua Shang*;Z. Fu

Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis

新型水杨酸类似物在拟南芥中诱导有效的防御反应

DOI：
10.3390/ijms20133356
发表时间：
2019
期刊：
International Journal of Molecular Sciences
影响因子：
5.6
作者：
Palmer, Ian Arthur;Chen, Huan;Chen, Jian;Chang, Ming;Li, Min;Liu, Fengquan;Fu, Zheng Qing
通讯作者：
Fu, Zheng Qing

PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity

PBS3 保护 EDS1 免受植物免疫中蛋白酶体介导的降解