Creating Dynamic and Adaptive Force-Producing Nanostructures

创建动态和自适应力产生纳米结构

• 批准号: 1807514
• 负责人: Henry Hess
• 金额: $ 46.32万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807514&HistoricalAwards=false
• 关键词: Creating; Dynamic; Adaptive; Force; Producing

Creating dynamic and adaptive force-producing nanostructuresNon-Technical Abstract: The heart muscle can operate reliably for more than a hundred years, but the molecules which generate the force to contract are replaced every few days. Muscle also responds to exercise with growth and will shrink if not used. Clearly, nature has developed ways to produce force very reliably and efficiently by dynamically organizing the molecules in this biological material. The goal of the proposed project is to take the first steps towards creating such materials in the laboratory. To this end, this project will investigate the approaches used by nature in the construction of muscles, and translate them to the engineering of man-made materials assembled from the same type of molecules used in muscles. The goal is to show that structures can be created "similar to muscle" that can be long-lived by automatically replacing their aging parts and can adapt to changing conditions by adding and releasing parts as needed. Compared to the traditional engineering approach where, for example, a long-lasting car is assembled from even more durable components, this bio-inspired approach may offer great benefits for engineering. At the same time it will help us to better understand the natural way of constructing muscles and other tissues. This understanding in turn can contribute to efforts to combat aging and restore bodily functions in medicine. In addition to obtaining new research insights, the aim is to inspire and train students at all levels. For example, teams of high school and undergraduate students will be mentored to compete in the BIOMOD biomolecular design competition.Technical Abstract: Future active materials that assemble molecular motors into structures with macroscopic force output will have to be engineered based on self-organization principles supporting the extraction of work, constant regeneration, and adaptation. The goal of this project is to discover these principles and investigate their application in a minimal model system utilizing motor proteins as the force- producing molecular components. In order to maintain these active nanostructures in a functional state beyond the limited lifetime of their active molecular components, this project will abstract the mechanisms which allow biological materials to maintain their function for a duration far beyond the lifetime of the components. In particular the roles of self-organization and continuous turnover, and the balance between desired structural stability and ease of component replacement will be investigated. The specific objectives of this research are (1) to create a structure in dynamic equilibrium where microtubules are propelled by kinesin motors weakly and reversibly bound to a surface, (2) to demonstrate that adaptation to changing loading conditions is possible due to the dynamic equilibrium established, and (3) to demonstrate that the dynamic recruitment of kinesin motors to microtubules lowers the number of motors required for stable microtubule gliding relative to a static configuration where motors are permanently adhered everywhere on the surface. The insights from these experiments will in return assist in developing a theoretical understanding of how biological materials both prevent and heal damage, and will be generalizable for the design of novel synthetic and hybrid materials. The project will integrate undergraduate and high school students in the research process and aim to recruit graduate students from underrepresented groups. Various outreach activities to local K-12 students are planned. The outcome of the project will be potentially transformational in that it aims to assist in a paradigm shift from durable man-made structures assembled from equally durable nanoscale components to durable and adaptive engineered structures composed of short-lived nanoscale components with continuous replacement.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

摘要：心肌可以可靠地运行一百多年，但产生收缩力的分子每隔几天就会被替换。肌肉也会随着运动而生长，如果不使用就会萎缩。显然，大自然通过动态组织这种生物材料中的分子，已经开发出了非常可靠和有效地产生力的方法。这个项目的目标是迈出在实验室中制造这种材料的第一步。为此，该项目将研究自然界在肌肉构造中使用的方法，并将其转化为由肌肉中使用的相同类型分子组装而成的人造材料的工程。其目标是证明可以创造出“类似于肌肉”的结构，这种结构可以通过自动替换老化的部分来延长寿命，并可以通过根据需要增加和释放部分来适应不断变化的条件。与传统的工程方法相比，例如，一辆经久耐用的汽车是由更耐用的部件组装而成的，这种生物启发的方法可能为工程提供了巨大的好处。同时，它将帮助我们更好地理解构建肌肉和其他组织的自然方式。这种理解反过来又有助于医学上对抗衰老和恢复身体功能的努力。除了获得新的研究见解外，其目的是激励和培养各级学生。例如，将指导高中生和大学生组成的团队参加BIOMOD生物分子设计竞赛。技术摘要：未来将分子马达组装成具有宏观力输出结构的活性材料必须基于支持功提取、不断再生和自适应的自组织原理进行设计。这个项目的目标是发现这些原理，并研究它们在利用运动蛋白作为产生力的分子成分的最小模型系统中的应用。为了使这些活性纳米结构在活性分子成分有限的寿命之外保持功能状态，该项目将抽象出允许生物材料在远远超过其成分寿命的时间内保持其功能的机制。特别是自组织和持续周转的作用，以及期望的结构稳定性和易于更换部件之间的平衡将被研究。本研究的具体目标是(1)创造一种动态平衡结构，其中微管由马达驱动，弱且可逆地绑定在表面上，(2)证明由于建立了动态平衡，适应不断变化的负载条件是可能的。(3)与静态配置（电机永久粘附在表面上的任何地方）相比，动态招募马达到微管降低了稳定微管滑动所需的马达数量。从这些实验中获得的见解将有助于从理论上理解生物材料如何预防和治愈损伤，并将推广到新型合成和混合材料的设计中。该项目将在研究过程中整合本科生和高中生，并旨在从代表性不足的群体中招募研究生。计划为当地K-12学生举办各种外展活动。该项目的结果将具有潜在的变革性，因为它旨在帮助实现从由同样耐用的纳米级组件组装的耐用人造结构到由寿命短的纳米级组件组成的耐用和适应性工程结构的范式转变。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Power Law Behavior in Protein Desorption Kinetics Originating from Sequential Binding and Unbinding

源自顺序结合和解结合的蛋白质解吸动力学中的幂律行为

• DOI: 10.1021/acs.langmuir.0c02260
• 发表时间: 2020
• 期刊: Langmuir
• 影响因子: 3.9
• 作者: Armstrong, Megan J.;Rodriguez, Juan B.;Dahl, Peter;Salamon, Peter;Hess, Henry;Katira, Parag
• 通讯作者: Katira, Parag

Actuating macroscopic machines with nanoscopic engines

用纳米引擎驱动宏观机器

• DOI: 10.1016/j.matt.2021.03.001
• 发表时间: 2021
• 期刊: Matter
• 影响因子: 18.9
• 作者: Zhang, Yifei;Hess, Henry
• 通讯作者: Hess, Henry

Molecular motors in materials science

材料科学中的分子马达

• DOI: 10.1557/mrs.2019.19
• 发表时间: 2019
• 期刊: MRS Bulletin
• 影响因子: 5
• 作者: Hess, Henry;Katira, Parag;Riedel-Kruse, Ingmar H.;Tsitkov, Stanislav
• 通讯作者: Tsitkov, Stanislav

Robotic end-to-end fusion of microtubules powered by kinesin

• DOI: 10.1126/scirobotics.abj7200
• 发表时间: 2021-11
• 期刊: Science Robotics
• 影响因子: 25
• 作者: Gadiel Saper;S. Tsitkov;Parag Katira;H. Hess

Muscle on demand

按需增肌

• DOI: 10.1038/s41563-021-01069-1
• 发表时间: 2021
• 期刊: Nature Materials
• 影响因子: 41.2
• 作者: Hess, Henry