Instrument Development: 4-D Super Time Resolved Microscopy (4-D STReM) for Understanding Dynamics in Porous Materials

仪器开发：用于了解多孔材料动力学的 4-D 超级时间分辨显微镜 (4-D STReM)

• 批准号: 1808382
• 负责人: Christy Landes
• 金额: $ 46.63万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1808382&HistoricalAwards=false
• 关键词: Instrument; Development; Super; Time; Resolved

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Landes at Rice University is working to understand and optimize processes that occur within porous materials. The goal of the project is to develop a new type of microscope with unprecedented space and time resolution. The Landes group's new microscope allows the study of how rare events impact the efficiency of porous materials that are important for catalysis, separations science, corrosion, and biology. It has been established that it is possible to manipulate light as it interacts with molecules and proteins. For example, Professor Landes has already shown that by shaping light's phase, events faster than the camera frame rate can be imaged. By incorporating new mathematical and physical tools, the current project will result in a new instrument to image and track fast dynamics in porous materials with optimized 3-D space and time resolution. The interdisciplinary nature of this research effort provides participating students with a unique experience at the interface of spectroscopy and materials science, as well as image processing and modern information theory, and continues the strong history of cross-disciplinary activities in science and technology at Rice University. This grant supports Professor Landes to provide training opportunities to high school teachers to incorporate cutting edge science into their course materials, as well as her new effort to create a summer scientific programming course. Recently, a new microscopy technique called super temporal-resolved microscopy (STREM) was developed. Proof-of-concept measurements showed that STREM can improve the time resolution of traditional wide-field cameras by at least twenty times. This development, if combined with recent advances in 3-D imaging methods and signal processing, represents an opportunity to resolve the multiscale, nonlinear dynamics that drive a range of interfacial materials properties. Thus, the current project's objective is to develop and optimize 4-D STREM, a chemical imaging method for quantifying the nonlinear dynamics and structures in porous materials. It is hypothesized that better 3-D sub-diffraction spatial information, coupled with improved time resolution and signal processing algorithms, reveals heterogeneous mass transport, chemical, and biological mechanisms occurring at porous interfaces. The project will involve innovations in both hardware and software to improve the temporal and 2-D spatial resolution. Additionally, a new algorithm is to be developed to track in 3-D. Finally, the new microscope is to be used to acquire and curate a machine learning library capable of differentiating among common analyte, sample, and instrument conditions. A new instrument optimized for characterizing the multiscalar physics and chemistry that underlie separations in porous media, by improving both spatial and temporal resolution is obtained in this project. Further, the project will result in new algorithms to extract information from large 3-D data sets. In terms of applications, a more detailed description of mass transport in pores and channels is a step towards predictive separations, which are currently optimized empirically, amounting to billions of dollars each year for industry, government, and academic purposes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像项目的支持下，莱斯大学的Landes教授正在努力了解和优化多孔材料中发生的过程。该项目的目标是开发具有前所未有的空间和时间分辨率的新型显微镜。Landes集团的新显微镜允许研究罕见事件如何影响多孔材料的效率，这些材料对催化，分离科学，腐蚀和生物学非常重要。已经确定，当光与分子和蛋白质相互作用时，可以操纵光。例如，Landes教授已经证明，通过对光的相位进行整形，可以成像速度超过相机帧速率的事件。通过结合新的数学和物理工具，目前的项目将产生一种新的仪器，以优化的三维空间和时间分辨率成像和跟踪多孔材料中的快速动态。这项研究工作的跨学科性质为参与的学生提供了光谱学和材料科学以及图像处理和现代信息理论的独特体验，并延续了莱斯大学科学和技术跨学科活动的悠久历史。这笔赠款支持教授兰德斯提供培训机会，以高中教师将尖端科学纳入他们的课程材料，以及她的新努力，创造一个夏季科学编程课程。最近，一种新的显微镜技术称为超时间分辨显微镜（斯特雷姆）的发展。概念验证测量表明，斯特雷姆可以将传统宽视场相机的时间分辨率提高至少20倍。这一发展，如果结合在3-D成像方法和信号处理的最新进展，代表了一个机会，以解决多尺度，非线性动力学，驱动一系列的界面材料的性能。因此，目前的项目的目标是发展和优化4-D斯特雷姆，一种用于定量多孔材料中的非线性动力学和结构的化学成像方法。据推测，更好的3-D子衍射空间信息，再加上改进的时间分辨率和信号处理算法，揭示了在多孔界面发生的异质质量传输，化学和生物机制。该项目将涉及硬件和软件方面的创新，以提高时间和二维空间分辨率。此外，一个新的算法是要开发的跟踪在3-D。最后，新显微镜将用于获取和管理能够区分常见分析物、样品和仪器条件的机器学习库。在这个项目中，通过提高空间和时间分辨率，获得了一种新的仪器，用于表征多孔介质中分离的多标量物理和化学。此外，该项目还将产生从大型三维数据集中提取信息的新算法。在应用方面，更详细地描述孔隙和通道中的物质传输是迈向预测分离的一步，目前这是根据经验优化的，每年为工业、政府和学术目的提供数十亿美元的资金。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Imaging Switchable Protein Interactions with an Active Porous Polymer Support

• DOI: 10.1021/acs.jpcb.0c01807
• 发表时间: 2020-06-04
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY B
• 影响因子: 3.3
• 作者: Dutta, Chayan;Bishop, Logan D. C.;Landes, Christy F.
• 通讯作者: Landes, Christy F.

A mechanistic examination of salting out in protein–polymer membrane interactions

• DOI: 10.1073/pnas.1909860116
• 发表时间: 2019-10
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Nicholas A Moringo;Logan D. C. Bishop;Hao Shen;Anastasiia Misiura;Nicole C. Carrejo;Rashad Baiyasi

Generalized method to design phase masks for 3D super-resolution microscopy

设计 3D 超分辨率显微镜相位掩模的通用方法

• DOI: 10.1364/oe.27.003799
• 发表时间: 2019
• 期刊: Optics Express
• 影响因子: 3.8
• 作者: Wang, Wenxiao;Ye, Fan;Shen, Hao;Moringo, Nicholas A.;Dutta, Chayan;Robinson, Jacob T.;Landes, Christy F.
• 通讯作者: Landes, Christy F.

Acoustic Vibrations of Al Nanocrystals: Size, Shape, and Crystallinity Revealed by Single-Particle Transient Extinction Spectroscopy

• DOI: 10.1021/acs.jpca.0c01190
• 发表时间: 2020-05-14
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY A
• 影响因子: 2.9
• 作者: Ostovar, Behnaz;Su, Man-Nung;Link, Stephan
• 通讯作者: Link, Stephan

Toward Protein Chromatography by Design: Stochastic Theory, Single-Molecule Parameter Control, and Stimuli-Responsive Materials

通过设计实现蛋白质色谱：随机理论、单分子参数控制和刺激响应材料

• DOI: 10.1021/acs.jpcc.2c05887
• 发表时间: 2022
• 期刊: The Journal of Physical Chemistry C
• 作者: Dutta, Chayan;Misiura, Anastasiia;Bishop, Logan D.;Marciel, Amanda B.;Kisley, Lydia;Landes, Christy F.
• 通讯作者: Landes, Christy F.