Regulating Nitric Oxide Homeostasis and its Impact on Plant Growth and Reproduction
调节一氧化氮稳态及其对植物生长和繁殖的影响
基本信息
- 批准号:1817985
- 负责人:
- 金额:$ 80万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project will determine how nitric oxide (NO) modulates important aspects of plant growth, development and seed yield, processes critical to food security and plant biomass production. NO acts as a signaling molecule in all higher organisms, and the discoverers of the role of NO in cells were awarded the Nobel Prize. This project will use biochemical and genetic approaches in Arabidopsis thaliana to investigate how a specific protein enzyme, known as GSNOR, controls the amount and effects of NO in plants. Results will contribute to our overall understanding of the mechanism of NO action in all organisms. One postdoctoral researcher, a graduate student, two undergraduates and a middle school teacher will receive training in laboratory research, as well as mentoring in written and oral communication of scientific data for specialist and lay audiences. In addition, outreach programs will focus on engaging teachers and students in Amherst middle and high schools with the goals of developing hands on experiments for middle school students involving plant materials and exposing high school students to University of Massachusetts STEM undergraduate majors and their campus experiences. The engagement with Amherst schools will also serve the important goal of strengthening community ties with the university.Despite the clear involvement of nitric oxide (NO) in multiple plant processes, including germination, root growth and fertility, a basic understanding of the mechanisms by which NO exerts its effects on systems critical for plant growth and development is lacking and will be addressed in this project. The cellular signaling and regulatory effects of NO are mediated by reversible, posttranslational protein redox modifications, including cysteine (Cys) nitrosation (Cys-SNO) and glutathionylation (Cys-SG). These reversible, covalent modifications result primarily from reaction with S-nitrosoglutathione (GSNO), a soluble NO-adduct of the abundant cellular redox buffer glutathione. They can have major effects on protein activity, indicating there must be mechanisms to control NO and GSNO concentrations in both time and space within cells. This project investigates how the enzyme S-nitrosoglutathione reductase (GSNOR) plays a major role in modulating GSNO and protein nitrosation levels. A model for regulation of GSNOR activity by nitrosation of specific Cys residues has been developed from biochemical studies of Arabidopsis, yeast and human GSNOR. This project will test this model both in vitro (Aim 1) and in vivo in Arabidopsis thaliana (Aim 2) using biochemistry and genetics, and possible regulation of GSNOR activity by other posttranslational modifications will be investigated. Experiments will also determine how GSNOR nitrosation is reversed by specific cytosolic redox proteins in the thioredoxin and thioredoxin reductase families (Aims 1 and 2). Results will develop a complete picture of the possible redox modifications controlling GSNOR activity and thereby cellular NO levels, and provide novel data on the activity and specificity of cytosolic thioredoxins. Finally, changes in protein nitrosation associated with the reduced fertility of Arabidopsis GSNOR null mutants will be determined by novel mass spectrometry methods (Aim 3). In total, experiments address how mechanisms controlling activity of a single enzyme (GSNOR) are propagated to result in phenotypic changes at the whole organism level.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目将确定一氧化氮(NO)如何调节植物生长、发育和种子产量的重要方面,以及对粮食安全和植物生物量生产至关重要的过程。一氧化氮在所有高等生物中都是一种信号分子,发现一氧化氮在细胞中的作用的人获得了诺贝尔奖。该项目将利用拟南芥的生化和遗传方法来研究一种被称为GSNOR的特定蛋白酶如何控制植物中NO的数量和作用。结果将有助于我们全面了解一氧化氮在所有生物体中的作用机制。一名博士后研究员、一名研究生、两名本科生和一名中学教师将接受实验室研究方面的培训,并指导专家和非专业观众进行科学数据的书面和口头交流。此外,外展计划将重点关注阿默斯特初中和高中的教师和学生,目标是为中学生开发涉及植物材料的动手实验,并让高中生了解马萨诸塞大学STEM本科专业及其校园经历。与阿默斯特学院的合作也将有助于加强社区与大学的联系。尽管一氧化氮(NO)明显参与植物的多个过程,包括发芽、根系生长和肥力,但对NO对植物生长发育至关重要的系统施加影响的机制的基本理解尚缺乏,这将在本项目中得到解决。NO的细胞信号传导和调节作用是通过可逆的翻译后蛋白氧化还原修饰介导的,包括半胱氨酸(Cys)亚硝化(Cys- sno)和谷胱甘肽化(Cys- sg)。这些可逆的共价修饰主要是与s -亚硝基谷胱甘肽(GSNO)反应产生的,GSNO是丰富的细胞氧化还原缓冲物谷胱甘肽的可溶性no加合物。它们可以对蛋白质活性产生重大影响,这表明一定存在控制细胞内时间和空间上NO和GSNO浓度的机制。本项目研究s -亚硝基谷胱甘肽还原酶(GSNOR)如何在调节GSNO和蛋白质亚硝化水平中发挥主要作用。从拟南芥、酵母和人GSNOR的生化研究中,建立了一个通过亚硝化特定的胱氨酸残基来调节GSNOR活性的模型。本项目将利用生物化学和遗传学方法在拟南芥(Arabidopsis thaliana)中体外(Aim 1)和体内(Aim 2)对该模型进行测试,并研究其他翻译后修饰对GSNOR活性的可能调节。实验还将确定硫氧还蛋白和硫氧还蛋白还原酶家族中的特定细胞质氧化还原蛋白如何逆转GSNOR亚硝化(目的1和2)。研究结果将为控制GSNOR活性和细胞NO水平的氧化还原修饰提供一个完整的图景,并为胞质硫氧还毒素的活性和特异性提供新的数据。最后,蛋白质亚硝化变化与拟南芥GSNOR零突变体生育能力降低相关,将通过新的质谱方法确定(目的3)。总的来说,实验解决了控制单一酶(GSNOR)活性的机制如何传播,从而导致整个生物体水平的表型变化。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Structural and biochemical characterization of Arabidopsis alcohol dehydrogenases reveals distinct functional properties but similar redox sensitivity
拟南芥醇脱氢酶的结构和生化特征揭示了不同的功能特性但相似的氧化还原敏感性
- DOI:10.1111/tpj.16651
- 发表时间:2024
- 期刊:
- 影响因子:0
- 作者:Meloni, Maria;Rossi, Jacopo;Fanti, Silvia;Carloni, Giacomo;Tedesco, Daniele;Treffon, Patrick;Piccinini, Luca;Falini, Giuseppe;Trost, Paolo;Vierling, Elizabeth
- 通讯作者:Vierling, Elizabeth
Corrigendum to New Phytologist 230 (2021), 2261–2274, doi: 10.1111/nph. 17152.
新植物学家勘误表 230 (2021), 2261–2274, doi: 10.1111/nph。
- DOI:
- 发表时间:2021
- 期刊:
- 影响因子:9.4
- 作者:Wang, J.;Guo, X.;Xiao, Q.;Zhu, J.;Cheung, A.Y.;Yuan, L.;Vierling, E.;Xu. S.
- 通讯作者:Xu. S.
Maternal nitric oxide homeostasis impacts female gametophyte development under optimal and stress conditions
母体一氧化氮稳态影响最佳和应激条件下雌配子体的发育
- DOI:10.1093/plcell/koae043
- 发表时间:2024
- 期刊:
- 影响因子:0
- 作者:Wang, Junzhe;Guo, Xiaolong;Chen, Yijin;Liu, Tianxiang;Zhu, Jianchu;Xu, Shengbao;Vierling, Elizabeth
- 通讯作者:Vierling, Elizabeth
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Elizabeth Vierling其他文献
Correction to: Structural and functional aspects of the interaction partners of the small heat-shock protein in Synechocystis
- DOI:
10.1007/s12192-018-0901-6 - 发表时间:
2018-05-03 - 期刊:
- 影响因子:3.200
- 作者:
Erik G. Marklund;Yichen Zhang;Eman Basha;Justin L. P. Benesch;Elizabeth Vierling - 通讯作者:
Elizabeth Vierling
Metabolic adaptation of wheat grain contributes to stable filling rate under heat stress
- DOI:
doi:10.1093/jxb/ery303 - 发表时间:
2018 - 期刊:
- 影响因子:
- 作者:
Wang Xiaoming;Hou Lijiang;Lu Yunze;Wu Bingjin;Gong Xue;Liu Manshuang;Wang Jun;Sun Qixin;Elizabeth Vierling;Xu Shengbao - 通讯作者:
Xu Shengbao
Cytoplasmic HSP70 homologues of pea: differential expression in vegetative and embryonic organs
- DOI:
10.1007/bf00019312 - 发表时间:
1995-02-01 - 期刊:
- 影响因子:3.800
- 作者:
Amy DeRocher;Elizabeth Vierling - 通讯作者:
Elizabeth Vierling
Conserved cell and organelle division
保守的细胞和细胞器分裂
- DOI:
10.1038/376473b0 - 发表时间:
1995-08-10 - 期刊:
- 影响因子:48.500
- 作者:
Katherine W. Osteryoung;Elizabeth Vierling - 通讯作者:
Elizabeth Vierling
Small heat shock proteins: multifaceted proteins with important implications for life
- DOI:
10.1007/s12192-019-00979-z - 发表时间:
2019-02-13 - 期刊:
- 影响因子:3.200
- 作者:
Serena Carra;Simon Alberti;Justin L. P. Benesch;Wilbert Boelens;Johannes Buchner;John A. Carver;Ciro Cecconi;Heath Ecroyd;Nikolai Gusev;Lawrence E. Hightower;Rachel E. Klevit;Hyun O. Lee;Krzysztof Liberek;Brent Lockwood;Angelo Poletti;Vincent Timmerman;Melinda E. Toth;Elizabeth Vierling;Tangchun Wu;Robert M. Tanguay - 通讯作者:
Robert M. Tanguay
Elizabeth Vierling的其他文献
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{{ truncateString('Elizabeth Vierling', 18)}}的其他基金
Collaborative Research: Defining functions of an essential, conserved protein that uniquely links the mitochondrial matrix with the cytoplasm
合作研究:定义一种重要的、保守的蛋白质的功能,该蛋白质将线粒体基质与细胞质独特地连接起来
- 批准号:
2215727 - 财政年份:2022
- 资助金额:
$ 80万 - 项目类别:
Standard Grant
Linking Reactive Nitrogen Metabolism and Redox Homeostasis in Plants
将植物中的活性氮代谢与氧化还原稳态联系起来
- 批准号:
1517046 - 财政年份:2015
- 资助金额:
$ 80万 - 项目类别:
Standard Grant
mTERF Function and Control of Plant Respiration and Stress Tolerance
mTERF 功能以及植物呼吸和胁迫耐受性的控制
- 批准号:
1354960 - 财政年份:2014
- 资助金额:
$ 80万 - 项目类别:
Continuing Grant
2007 Gordon Research Conference - Temperature Stress in Plants; Ventura, CA; January 21-26, 2007
2007 年戈登研究会议 - 植物的温度胁迫;
- 批准号:
0704051 - 财政年份:2007
- 资助金额:
$ 80万 - 项目类别:
Standard Grant
Function of the Small Hsps in Stress Tolerance, Growth and Development
小热休克蛋白在应激耐受、生长发育中的作用
- 批准号:
0213128 - 财政年份:2002
- 资助金额:
$ 80万 - 项目类别:
Continuing Grant
POWRE: Quantitative Trait Loci Controlling High Temperature Adaptation in Plants
POWRE:控制植物高温适应的数量性状基因座
- 批准号:
0074870 - 财政年份:2000
- 资助金额:
$ 80万 - 项目类别:
Standard Grant
POWRE: A Genetic Approach to Structure and Function Analysis of a New Class of Molecular Chaperones
POWRE:一类新型分子伴侣结构和功能分析的遗传方法
- 批准号:
9752978 - 财政年份:1997
- 资助金额:
$ 80万 - 项目类别:
Standard Grant
Expression and Structure of a Chloroplast-localized Heat Shock Protein
叶绿体定位热休克蛋白的表达和结构
- 批准号:
8517576 - 财政年份:1986
- 资助金额:
$ 80万 - 项目类别:
Continuing Grant
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Mechanism of epigenetic regulation by nitric oxide with a novel compound regulating the modification of cysteine residue
一氧化氮与调节半胱氨酸残基修饰的新型化合物的表观遗传调控机制
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Genetic evaluation of nitric oxide regulating genes in ischemic stroke
缺血性脑卒中一氧化氮调节基因的遗传评价
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- 资助金额:
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Genetic evaluation of nitric oxide regulating genes in ischemic stroke
缺血性脑卒中一氧化氮调节基因的遗传评价
- 批准号:
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- 批准号:
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Biochemical properties of S-nitroso-myoglobin and its role in regulating nitric oxide bio-availability
S-亚硝基肌红蛋白的生化特性及其在调节一氧化氮生物利用度中的作用
- 批准号:
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Discovery Projects
Biochemical properties of S-nitroso-myoglobin and its role in regulating nitric oxide bio-availability
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NOVEL MECHANISMS REGULATING NITRIC OXIDE IN THE SKIN
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