Biochemical properties of S-nitroso-myoglobin and its role in regulating nitric oxide bio-availability

S-亚硝基肌红蛋白的生化特性及其在调节一氧化氮生物利用度中的作用

• 批准号: DP0343325
• 负责人: Prof Paul Witting
• 金额: $ 50.66万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2007-11-06
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0343325
• 关键词: Biochemical; properties; nitroso; myoglobin; its

Nitric oxide (NO) stimulates blood vessel dilation. Vessel dilation is essential to maintaining blood pressure. Altered NO-regulation leads to vessel dysfunction. Within blood vessels, myoglobin regulates NO concentrations through oxidation and binding reactions. In contrast, S-nitroso-myoglobin represents a novel source of NO in humans. The goal of this study is to expand the knowledge on NO-regulation by myoglobin through determining S-nitroso-myoglobin's - chemical stability, rates of formation and decay, concentration in human vessels and whether it can cause blood vessel dilation similar to authentic NO. Such novel data represents a major fundamental advance in understanding the role of myoglobin in NO-homeostasis.

一氧化氮(NO)刺激血管扩张。血管扩张对维持血压至关重要。无调节的改变会导致血管功能障碍。在血管内，肌红蛋白通过氧化和结合反应调节NO浓度。相比之下，S-亚硝基-肌红蛋白代表了人类一氧化氮的一种新来源。本研究的目的是通过测定S-亚硝基-肌红蛋白的化学稳定性、形成和衰变速率、在人体血管中的浓度以及它是否能引起与正品NO类似的血管扩张，来扩大对肌红蛋白对NO调节作用的认识。这些新的数据代表了在理解肌红蛋白在非内稳态中的作用方面的重大基础性进展。