The role of reactive gliosis of Müller cells in retinal ischemia

Müller 细胞反应性神经胶质增生在视网膜缺血中的作用

• 批准号: 234236646
• 负责人: Professor Dr. Andreas Reichenbach
• 金额: --
• 依托单位: Abteilung Pathophysiologie der Neuroglia
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/234236646?language=en
• 关键词: role; reactive; gliosis; ller; cells

Dysfunction of blood supply (ischemia) of the nervous tissue including the retina may cause massive neuronal cell death and loss of function of the tissue. Müller glial cells, the dominating macroglial cell of the retina, undergo a reactive gliosis after retinal ischemia and other retinal injuries and diseases. This process is characterized by typical alterations in gene expression, morphology, and physiology. Gliosis is often called janus-faced, because these alterations may have beneficial as well as detrimental effects on the nervous tissue. The exact role of the glial reaction in the course of various neuronal diseases is still in the focus of research. In the current project, two animal models for retinal ischemia (transiently increased intraocular pressure and sustained closure of the central retinal artery) should be applied on different transgenic mice strains. These mice are lacking certain proteins or cellular functions which may be involved in the development of reactive gliosis. After ischemia, functional alterations of Müller cells and the degree of neuronal degeneration should be investigated in these mice. One aim of the study is to better understand the process of reactive gliosis in the central nervous system using the retinal Müller cell as a typical glial cell. This should help to develop therapeutical approaches for a selective manipulation of defined glial cell reactions, to achieve a positive influence on the postischemic cellular alterations.

包括视网膜在内的神经组织的血供功能障碍（缺血）可能导致大量神经元细胞死亡和组织功能丧失。视网膜大胶质细胞中占主导地位的神经胶质细胞，在视网膜缺血和其他视网膜损伤和疾病后发生反应性胶质细胞增生。这一过程的特点是基因表达、形态和生理上的典型改变。胶质瘤通常被称为双面，因为这些改变可能对神经组织有有益和有害的影响。胶质反应在各种神经疾病过程中的确切作用仍是研究的焦点。本项目拟在不同转基因小鼠品系上应用两种视网膜缺血动物模型（短暂性眼压升高和视网膜中央动脉持续关闭）。这些小鼠缺乏可能参与反应性胶质瘤发展的某些蛋白质或细胞功能。在缺血后，研究这些小鼠的神经细胞功能改变和神经元变性程度。该研究的目的之一是更好地了解中枢神经系统反应性胶质瘤的过程，利用视网膜<s:1> ller细胞作为典型的胶质细胞。这将有助于开发治疗方法，以选择性地操纵确定的胶质细胞反应，以实现对缺血后细胞改变的积极影响。

项目成果

Glutamate release from satellite glial cells of the murine trigeminal ganglion

• DOI: 10.1016/j.neulet.2014.06.047
• 发表时间: 2014-08
• 期刊: Neuroscience Letters
• 影响因子: 2.5
• 作者: Lysann Wagner;R. Warwick;T. Pannicke;A. Reichenbach;A. Grosche;M. Hanani

Retinal functional alterations in mice lacking intermediate filament proteins glial fibrillary acidic protein and vimentin

• DOI: 10.1096/fj.15-272963
• 发表时间: 2015-08
• 期刊: The FASEB Journal
• 作者: K. Wunderlich;N. Tanimoto;A. Grosche;E. Zrenner;M. Pekny;A. Reichenbach;M. Seeliger;T. Pannicke;Maria-Thereza R. Perez