Light Propagation through the Retina: Vertebrate Retinal Optics

光在视网膜中的传播：脊椎动物视网膜光学

• 批准号: 193205741
• 负责人: Professor Dr. Andreas Reichenbach
• 金额: --
• 依托单位: Abteilung Physik der weichen Materie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/193205741?language=en
• 关键词: Light; Propagation; through; Retina; Vertebrate

It is an old enigma how the vertebrate retina can function properly despite being inverted: light must pass all (light scattering) retinal layers before it can be detected by the photoreceptor cells. Recently we have shown that Müller radial glial cells act as light guiding fibers, bypassing the inner retinal layers. However, this light guidance must end at the outer nuclear layer where the Müller cell processes split into thin cytoplasmic tongues. Our collaborating partner has forwarded the hypothesis that the radial rows of photoreceptor cell nuclei serve as ‘chains of lenses’ bridging the distance between the Müller cell stem processes and the photoreceptor inner segments. To test this hypothesis and to elucidate the complex light path through the entire retina, we will perform (i) a quantitative analysis of the numerical relation between the subsequent light-guiding elements, (ii) a structural analysis of the ‘transition zone(s)’ between them, and (iii) a direct visualization of light transfer through the retina. For the latter purpose, laser light will be applied by a thin fiber to the inner surface of the retina. The light path of the laser beam will be monitored, as well as the fluorescence induced by it within the structures of the retina, labeled by fluorescent vital dyes. In collaboration with the partner in Cambridge, mathematical modeling of light transport through the retina will be performed.

脊椎动物的视网膜如何在倒置的情况下正常工作是一个古老的谜：光必须通过所有（光散射）视网膜层才能被感光细胞检测到。最近，我们已经表明，米勒放射状胶质细胞作为光导纤维，绕过视网膜内层。然而，这种光的引导必须终止于外核层，在那里米勒细胞突起分裂成薄的细胞质舌。我们的合作伙伴提出了这样的假设，即感光细胞核的放射状排列作为“透镜链”，桥接了Müller细胞干细胞突起和感光细胞内节之间的距离。为了验证这一假设并阐明通过整个视网膜的复杂光路，我们将执行（i）后续光导元件之间的数值关系的定量分析，（ii）它们之间的“过渡区”的结构分析，以及（iii）通过视网膜的光传输的直接可视化。对于后一个目的，激光将通过细纤维施加到视网膜的内表面。将监测激光束的光路，以及由其在视网膜结构内诱导的荧光，由荧光活性染料标记。与剑桥的合作伙伴合作，将对通过视网膜的光传输进行数学建模。

项目成果

Live Cells as Optical Fibers in the Vertebrate Retina

活细胞作为脊椎动物视网膜中的光纤

• DOI: 10.5772/26945
• 发表时间: 2012
• 作者: Reichenbach;K. Franze;S. Agte;S. Junek;A. Wurm;J. Grosche;A. Savvinov;J. Guck;S. N. Skatchkov
• 通讯作者: S. N. Skatchkov

Retinal adaptation to dim light vision in spectacled caimans (Caiman crocodilus fuscus): Analysis of retinal ultrastructure

• DOI: 10.1016/j.exer.2018.05.006
• 发表时间: 2018-08-01
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Karl, Anett;Agte, Silke;Bringmann, Andreas
• 通讯作者: Bringmann, Andreas

Unidirectional Photoreceptor-to-Müller Glia Coupling and Unique K+ Channel Expression in Caiman Retina

凯门鳄视网膜中单向光感受器与穆勒神经胶质细胞的耦合和独特的 K 通道表达

• DOI: 10.1371/journal.pone.0097155
• 发表时间: 2014
• 期刊: PLoS ONE
• 影响因子: 3.7
• 作者: Zayas-Santiago;S. Agte;Y.Rivera;J. Benedikt;E. Ulbricht;A. Karl;J. Dávila;A. Savvinov;Y. Kucheryavykh;M. Inyushin;L.A. Cubano;T. Pannicke;R.W. Veh;M. Francke;A. Verkhratsky;M.J. Eaton;A. Reichenbach;S.N. Skatchkov
• 通讯作者: S.N. Skatchkov