A Molecular Link Between Actin and Exocytosis

肌动蛋白和胞吐作用之间的分子联系

• 批准号: 1824636
• 负责人: Magdalena Bezanilla
• 金额: $ 8.71万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-01-02 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1824636&HistoricalAwards=false
• 关键词: Molecular; Link; Between; Actin; Exocytosis

Intellectual Merit: Polarized growth of individual cells is a complex process integrating cellular metabolism events and the extracellular environment. Extension and shape changes are frequently accompanied with deposition of material into their extracellular environments. In plants, cells also build a network of carbohydrate polymers that act as a rigid barrier, known as the cell wall. Plant cells grow by building new cell wall material and plant cell shape is intimately linked with how this material is deposited on the outside of the cell. This project will work to elucidate the link between delivery of cell wall material and the cell's internal network of dynamic filaments known as the cytoskeleton. In particular the PI will use sophisticated light microscope imaging methods to test the hypothesis that formin proteins are critical for targeting actin assembly processes that initiate cell wall material exocytosis. Although it is known that cell growth and the cytoskeleton are intimately connected, a detailed mechanistic understanding of this coupling remains to be elucidated. This project will take advantage of moss, a simple, genetically tractable plant model system, to address this fundamental cell biological question.Broader Impacts: The investigator will broaden the impacts of the proposed research by providing resources and expertise to the plant research community, training of undergraduates and underrepresented minorities, and incorporating aspects of the project in undergraduate education. The moss model system is ideally suited for undergraduate laboratory experiences. Therefore, the investigator will also develop a project-based laboratory course wherein students perform RNA-induced silencing experiments to analyze the function of individual genes and their roles in cell growth. Additionally, a summer laboratory module offered through the STEM Diversity Institute at U. Mass will aim to increase participation and recruitment of underrepresented minorities. Course materials from the summer module will be distributed to underrepresented faculty at partner institutions to facilitate implementation of similar courses. Incorporating faculty and students in this endeavor will contribute to increasing diversity in the sciences.

智力优势：单个细胞的极化生长是整合细胞代谢事件和细胞外环境的复杂过程。伸展和形状的变化经常伴随着物质沉积到细胞外环境中。在植物中，细胞还构建了一个碳水化合物聚合物网络，作为一个刚性屏障，称为细胞壁。植物细胞通过构建新的细胞壁材料而生长，植物细胞的形状与这种材料如何沉积在细胞外部密切相关。这个项目将致力于阐明细胞壁材料的传递和细胞内部动态细丝网络（称为细胞骨架）之间的联系。特别是PI将使用先进的光学显微镜成像方法来测试的假设，即α-淀粉样蛋白是关键的目标肌动蛋白组装过程，启动细胞壁材料胞吐。虽然它是已知的，细胞生长和细胞骨架是密切相关的，这种耦合的详细机制的理解仍有待阐明。该项目将利用苔藓，一个简单的，易于遗传的植物模型系统，以解决这一基本的细胞生物学问题。更广泛的影响：调查员将扩大拟议的研究的影响，提供资源和专业知识，植物研究界，本科生和代表性不足的少数民族的培训，并纳入本科教育项目的各个方面。moss模型系统非常适合本科生的实验室经验。因此，研究人员还将开发一个基于项目的实验室课程，学生进行RNA诱导的沉默实验，以分析单个基因的功能及其在细胞生长中的作用。此外，夏季实验室模块提供通过干多样性研究所在美国。Mass的目标是增加代表性不足的少数民族的参与和招聘。暑期单元的课程材料将分发给伙伴机构中任职人数不足的教员，以促进类似课程的实施。教师和学生在这奋进的努力将有助于增加科学的多样性。

项目成果

In vivo analysis of formin dynamics in the moss P. patens reveals functional class diversification

• DOI: 10.1242/jcs.233791
• 发表时间: 2020-01
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: Peter A C van Gisbergen;Shu-Zon Wu;Xiaohang Cheng;Kelli A. Pattavina;M. Bezanilla