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Understanding the link between the axilla microbiome bacteria and odorous thioalcohol generation at the molecular level

在分子水平上了解腋窝微生物组细菌与硫醇气味产生之间的联系

基本信息

批准号：
2599446
负责人：
金额：
--
依托单位：
University of Liverpool
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2021
资助国家：
英国
起止时间：
2021 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2599446
关键词：
Understanding link between axilla microbiome

项目摘要

It is widely accepted that axillary malodour is driven by the production of volatile fatty acids (VFAs), thioalcohols and steroids. These molecules are generated by the microbial biotransformation of odourless precursor molecules originated from and are secreted by the apocrine gland. As the molecules of interest can be highly volatile and small, they can be difficult to sample and analyse, leading to loss of valuable information, including quantification of the molecules, and making associations between malodourous thiols and the bacteria within the microbial population of the armpit which are producing these thiols. By being able to measure these volatile molecules, we can identify with bacteria generate malodorous thiols.The main aim of this project is to develop surface enhanced Raman scattering (SERS) to detect and quantify the thiols generated from the armpit. Alongside SERS, thermal desorption- gas chromatography - mass spectrometry (TD-GC-MS) will be used to profile volatile organic compounds and phylogenetic approaches will be used to define the axilla microbiome.By combining these techniques and examining the relationship between bacteria in the axillary microbiota and thioalcohol generation, the project aims to develop an in depth understanding of the axilla microbiome and quantify the key drivers of malodour.

人们普遍认为，腋窝恶臭是由挥发性脂肪酸（VFA）、硫醇和类固醇的产生引起的。这些分子是由来源于顶泌腺并由顶泌腺分泌的无味前体分子的微生物生物转化产生的。由于感兴趣的分子可能是高度挥发性的和小的，它们可能难以取样和分析，导致有价值的信息的损失，包括分子的定量，以及恶臭硫醇与腋窝微生物群体内产生这些硫醇的细菌之间的关联。通过测量这些挥发性分子，我们可以识别产生恶臭硫醇的细菌。本项目的主要目的是开发表面增强拉曼散射（Sers）来检测和定量腋窝产生的硫醇。除了Sers，热解吸-气相色谱-质谱（TD-GC-MS）将用于分析挥发性有机化合物，系统发育方法将用于定义腋窝微生物组。通过结合这些技术并检查腋窝微生物组中的细菌与硫醇生成之间的关系，该项目旨在深入了解腋窝微生物组，并量化恶臭的主要驱动因素。