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MRI: Development: Extreme Life Volumetric Imaging System (ELVIS)

MRI：开发：极限生命体积成像系统 (ELVIS)

基本信息

批准号：
1828793
负责人：
Jay Nadeau
金额：
$ 63.03万
依托单位：
Portland State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2024-02-29
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828793&HistoricalAwards=false
关键词：
MRI Development Extreme Life Volumetric

项目摘要

The objective of this project is to develop a unique multi-modal volumetric microscope capable of instantaneous imaging of sample volumes approximately 100-fold larger than those sampled by conventional fluorescence microscopy, with spatial resolution sufficient to detect most bacteria and archaea. This instrument will combine two modalities for the first time: Digital Holography Microscopy (DHM) (which includes amplitude [brightfield] and quantitative phase imaging) and Fluorescence Light Field Microscopy (FLFM). The Co-PIs have established each technology separately as research instruments in their laboratories; this will be the first time that a microscope with all of the described capabilities will be made available for research. The instrument that will be build will be packaged for field use in aquatic environments. It will be used in the lab, and also made available to researchers. PSU is undergoing a major renovation of its research infrastructure, plan, and long-term outlook, which includes a growing interest in STEM and a major expansion in PhD programs. This instrument will enable research and training across academic levels, from high school through PhD, and will be an important part of PSU's growing research profile and engagement with other institutions and the community. The PI has an established pattern of public outreach and training; two areas of particular excellence are (1) engaging undergraduates in research, with a particular emphasis on recruiting women and underrepresented minorities, including English language learners; and (2) developing curriculum and training programs for STEM teachers. This instrument will assist these efforts, as well as new efforts, specifically: addition of a laboratory component to the existing junior/senior/graduate-level Cellular and Molecular Biophysics course; creation of a new Senior Capstone field course; participation in the NASA "Spaceward Bound" teachers' workshops.The first combined volumetric brightfield, phase, and fluorescence imaging system, will be created by coupling holographic and light field microscopy. This will enable instantaneous volumetric imaging, which has a wide variety of emerging and potential applications in cell, developmental, and organismal biology, as well as in the physical sciences (hydrology, materials science). Conventional microscopy requiring sectioning (confocal or multiphoton) is too slow to capture a large number of kinetic events: bacterial swimming, heartbeats, blood flow, intracellular organelle movement, and many more. The limitations of conventional imaging modalities could be mitigated if one could record the spatial information of an extended sample volume in a single camera snapshot. Such synchronous volumetric imaging capability not only removes the delicate focusing requirement of conventional microscopy, but also dramatically enhances the imaging throughput. In this project, the PI will tailor the instrument to in situ environmental microbiology of remote or extreme environments. Micron-scale organisms remain a neglected size scale for which limited in situ imaging has been performed. Specific target areas for ELVIS include the open ocean, sea ice, glacier ice, and hydrothermal vents. A dozen interested users from different institutions have been identified.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目的目标是开发一种独特的多模态体积显微镜，能够瞬时成像的样品体积大约是传统荧光显微镜取样的100倍，具有足够的空间分辨率来检测大多数细菌和古细菌。该仪器将首次结合两种模式：数字全息显微镜（DHM）（包括振幅[明场]和定量相位成像）和荧光光场显微镜（FLFM）。各合作项目在其实验室中将每项技术分别确定为研究工具；这将是第一次将具有上述所有功能的显微镜用于研究。将要建造的仪器将被包装起来，以便在水生环境中实地使用。它将在实验室使用，也可供研究人员使用。PSU正在对其研究基础设施、计划和长期前景进行重大改造，其中包括对STEM日益增长的兴趣和博士课程的重大扩展。该工具将实现从高中到博士的跨学术水平的研究和培训，并将成为PSU不断增长的研究形象和与其他机构和社区合作的重要组成部分。PI有既定的公共宣传和培训模式；两个特别优秀的领域是：(1)让本科生参与研究，特别强调招募女性和代表性不足的少数民族，包括英语学习者；(2)为STEM教师制定课程和培训计划。该仪器将有助于这些努力，以及新的努力，特别是：在现有的大三/大四/研究生水平的细胞和分子生物物理学课程中增加实验室部分；创建新的高级顶点课程；参加美国国家航空航天局“向太空进发”教师讲习班。第一个结合体明场，相位和荧光成像系统，将创建耦合全息和光场显微镜。这将使瞬时体积成像成为可能，它在细胞、发育和有机生物学以及物理科学（水文学、材料科学）中具有广泛的新兴和潜在应用。需要切片的常规显微镜（共聚焦或多光子）速度太慢，无法捕捉到大量的动力学事件：细菌游泳、心跳、血液流动、细胞内细胞器运动等等。如果可以在单个相机快照中记录扩展样本量的空间信息，则可以减轻传统成像方式的局限性。这种同步体成像能力不仅消除了传统显微镜对精细聚焦的要求，而且大大提高了成像吞吐量。在这个项目中，PI将调整仪器以适应偏远或极端环境的原位环境微生物学。微米级生物仍然是一个被忽视的尺寸尺度，有限的原位成像已经进行。ELVIS的具体目标区域包括公海、海冰、冰川冰和热液喷口。已经确定了来自不同机构的十几个感兴趣的用户。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

ELVIS：用于现场和实验室研究的相关光场和数字全息显微镜 – 现场演示

DOI：
10.1017/s1551929520001133
发表时间：
2020
期刊：
Microscopy Today
影响因子：
0
作者：
Kim, Taewoo;Serabyn, Eugene;Liewer, Kurt;Oborny, Nathan;Wallace, J. Kent;Rider, Stephanie;Bedrossian, Manuel;Lindensmith, Christian;Nadeau, Jay
通讯作者：
Nadeau, Jay

A Multi-modal Volumetric Microscope with Automated Sample Handling for Surveying Microbial Life in Liquid Samples

具有自动样品处理功能的多模式体积显微镜，用于测量液体样品中的微生物生命

DOI：
10.3389/fspas.2022.763329
发表时间：
2022
期刊：
Frontiers in astronomy and space sciences
影响因子：
3
作者：
Kim, Taewoo;Oborny, Nathan;Serabyn, Eugene;Wallace, J. Kent;Liewer, Kurt;Bedrossian, Manuel;Rider, Stephanie;Noell, Aaron;Willis, Peter;Lindensmith, Chris
通讯作者：
Lindensmith, Chris

ELVIS：用于现场和实验室研究的相关光场和数字全息显微镜

DOI：
10.1017/s1551929520000899
发表时间：
2020
期刊：
Microscopy Today
影响因子：
0
作者：
Kim, Taewoo;Serabyn, Eugene;Schadegg, Maximilian;Liewer, Kurt;Oborny, Nathan;Kent Wallace, J.;Rider, Stephanie;Bedrossian, Manuel;Lindensmith, Christian;Nadeau, Jay
通讯作者：
Nadeau, Jay

Multiwavelength Digital Holographic Imaging and Phase Unwrapping of Protozoa Using Custom Fiji Plug-ins