RoL: FELS: EAGER: Emergent Functions of Secreted Microbial Effectors

这项研究将使可持续农业和粮食安全取得重大进展。 该项目研究了致病微生物如何部署分子组来破坏植物。收集到的信息将用于大学课程开发。此外，还将通过同行评审的出版物向科学家传播，并通过公共社区节日的讨论向公众传播。不同的学生将接受尖端的培训，因为他们的工作，以实现该项目的目标。细菌植物病原体转移到植物效应蛋白，形成物理和功能网络，抑制植物防御，从而损害植物。一个新的观点是效应物在多个隔室（微生物和宿主）中相互相遇。效应子联合体的涌现功能包括效应子之间相互作用产生的相加和拮抗行为，其中一些导致翻译后修饰（PTM）。本研究的目标是确定效应器网络生物化学相互作用事件是否发生在细菌和植物中，并进一步表征网络相互作用，重点是效应器是否形成高阶复合物。该方法包括质谱检测效应器催化的PTM和结合实验，以调查效应器-效应器的相互作用和PTM对他们的影响。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

This research will enable important advances in sustainable agriculture and food security to be achieved. The project investigates how disease-causing microbes deploy groups of molecules to damage plants. The information gleaned will be used for college curriculum development. Furthermore, it will be disseminated to scientists through peer-reviewed publications and the general public through discussions at public community festivals. Diverse students will receive cutting edge training as they work to achieve the goals of the project.Bacterial plant pathogens translocate into plants effector proteins that form physical and functional networks that suppress plant defenses and thus damage plants. A new perspective is that effectors encounter each other in multiple compartments (microbe and host). Emergent functions of effector consortia include additive and antagonistic behaviors that arise from interactions between the effectors, some of which lead to posttranslational modifications (PTMs). The goal of this research is to determine whether effector network biochemical interactions events occur in bacteria as well as in plants, and to further characterize network interactions, with a focus on whether effectors form higher order complexes. The approaches include mass spectrometry to detect effector-catalyzed PTMs and binding experiments to investigate effector-effector interactions and the impact of PTMs on them.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.