RoL: FELS: EAGER: Emergent Functions of Secreted Microbial Effectors

这项研究将使可持续农业和粮食安全方面取得重要进展。该项目调查致病微生物如何部署分子群来破坏植物。收集到的信息将用于大学课程开发。此外，它将通过同行评议的出版物向科学家传播，并通过在公共社区节日上的讨论向公众传播。不同的学生将接受尖端培训，以实现该项目的目标。细菌植物病原体转移到植物效应器蛋白质中，形成物理和功能网络，抑制植物防御，从而损害植物。一种新的观点是，效应器在多个隔间(微生物和宿主)中相遇。效应器联合体的紧急功能包括由效应器之间的相互作用产生的相加和拮抗行为，其中一些行为导致翻译后修饰(PTM)。本研究的目的是确定效应器网络生化相互作用事件是否发生在细菌和植物中，并进一步表征网络相互作用，重点是效应器是否形成更高阶的复合体。这些方法包括检测效应器催化的PTM的质谱学和调查效应器-效应器相互作用和PTM影响的绑定实验。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This research will enable important advances in sustainable agriculture and food security to be achieved. The project investigates how disease-causing microbes deploy groups of molecules to damage plants. The information gleaned will be used for college curriculum development. Furthermore, it will be disseminated to scientists through peer-reviewed publications and the general public through discussions at public community festivals. Diverse students will receive cutting edge training as they work to achieve the goals of the project.Bacterial plant pathogens translocate into plants effector proteins that form physical and functional networks that suppress plant defenses and thus damage plants. A new perspective is that effectors encounter each other in multiple compartments (microbe and host). Emergent functions of effector consortia include additive and antagonistic behaviors that arise from interactions between the effectors, some of which lead to posttranslational modifications (PTMs). The goal of this research is to determine whether effector network biochemical interactions events occur in bacteria as well as in plants, and to further characterize network interactions, with a focus on whether effectors form higher order complexes. The approaches include mass spectrometry to detect effector-catalyzed PTMs and binding experiments to investigate effector-effector interactions and the impact of PTMs on them.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.