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Biomedical sensor platform based on optical microresonator array

基于光学微谐振器阵列的生物医学传感器平台

基本信息

批准号：
235490255
负责人：
Professor Dr.-Ing. Andreas Ostendorf
金额：
--
依托单位：
Lehrstuhl für Laseranwendungstechnik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/235490255?language=en
关键词：
Biomedical sensor platform based optical

项目摘要

Within this project, an innovative microsensor platform should be established and tested, which combines in a simple way the high sensitivity of optical microresonators with the selectivity of biochemical processes, like antigene-antibody reactions. The sensor platform consists of a replaceable sensor chip and the readout unit. The sensor chip contains an array of optical microresonators with slightly different sizes. The particles are coated with a selective layer. Illuminated with laser light, some particles show optical resonances which depend strongly on the ratio between the applied wavelength and the diameter of the particle. Some particles are partly in resonance and some are off resonance. The particles radiate therefore with different brightness. This forms a characteristic light intensity pattern. Changes of the surface, e.g. due to adsorption of substances on the surface of the particle, lead to a change of the resonance properties and therefore of the light intensity pattern. The specific coating provides a selective adsorption of the analyte. This makes it possible to detect the analyte. The pattern is recorded by a CCD-camera and processed by a connected PC. The properties of the sensor are investigated exemplarily by means of the pair Streptavidin (as coating) and Biotin (as analyte). With this platform, it should be possible to detect substance quantities e.g. biotin in the range of 1 nM.

在该项目中，应建立和测试一个创新的微传感器平台，该平台以简单的方式将光学微谐振器的高灵敏度与生物化学过程的选择性相结合，如抗原抗体反应。传感器平台由可更换的传感器芯片和读出单元组成。该传感器芯片包含一个阵列的光学微谐振器略有不同的大小。这些颗粒被一层选择性的涂层所覆盖。用激光照射，一些颗粒显示出光学共振，这强烈依赖于所施加的波长和颗粒直径之间的比率。有些粒子部分处于共振状态，有些则处于非共振状态。因此，粒子以不同的亮度辐射。这形成了特征光强度图案。例如由于物质在颗粒表面上的吸附而引起的表面的变化导致共振特性的变化，并因此导致光强度图案的变化。特定涂层提供分析物的选择性吸附。这使得可以检测分析物。图案由CCD摄像机记录并由连接的PC处理。传感器的特性通过链霉亲和素（作为涂层）和生物素（作为分析物）对进行示例性研究。使用该平台，应该可以检测1 nM范围内的物质量，例如生物素。