CSBR: Living Stocks, Operation of a Culture Collection of Algae

CSBR：活畜、藻类培养物保藏库的运营

• 批准号: 1755220
• 负责人: David Nobles
• 金额: $ 47.7万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-15 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1755220&HistoricalAwards=false
• 关键词: CSBR; Living; Stocks; Operation; Culture

Algae are essential primary producers that profoundly affect the global environment, ecology, and economy. The Culture Collection of Algae at the University of Texas at Austin (UTEX) is one of only a handful of large biodiversity collections of living algae. UTEX maintains more than 3,000 distinct algal strains and is among the most genetically diverse assemblages of living organisms available to the public. Many of the algae in the UTEX Collection are unique and irreplaceable. UTEX distributes thousands of living algal cultures to scientists, educators, and students throughout the U.S. and the world. Great interest in algae as sources of transportation fuels, human food, animal feed, pharmaceuticals, and many other uses makes UTEX cultures and services essential resources for researchers. Every year, several hundred published research articles, many of which recognize NSF support, reference the use of UTEX strains. UTEX provides extensive student training, hands-on workshops on managing algal cultures, an informative interactive web site (https://utex.org/), species identification services, chemical composition analysis, and extensive correspondence with students engaged in algal research. UTEX personnel give presentations at public schools, conduct tours of UTEX facilities, assist teachers and students in conducting high school science projects, and provide presentations on the scientific importance of algae to hundreds of school children at local open house events.UTEX is a public resource whose mission is to promote, support, and enable the use of algae for research, education, and practical applications. The primary goals of UTEX that fulfill this mission are (1) maintaining a well-curated permanent repository for algal biodiversity that insures the continuity and consistency of algal research; (2) providing living algal cultures and related materials to the user community at a modest cost; (3) acting as a source of information regarding algae to researchers, educators, and the interested public; and (4) participating in outreach to the user community through training, support services, and supplies. Additional goals of this project are to (1) enhance the security of UTEX holdings through improved culture maintenance techniques and expansion of the number of strains in cryogenic storage, (2) increase the value of UTEX algal strains to the user community by characterizing their genomic and metabolic diversity, (3) expand interactions with K-12 and undergraduate education programs through the development of teaching kits and online teaching tools, and (4) accession and make available a greater diversity of strains that are of value for emerging and expanding areas of pure and applied research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

藻类是重要的初级生产者，深刻影响着全球环境、生态和经济。德克萨斯大学奥斯汀分校(UTEX)的藻类培养收藏是为数不多的生物多样性活藻类大型收藏之一。UTEX保存着3000多种不同的藻类，是公众可用的最具遗传多样性的生物组合之一。UTEX收藏中的许多藻类都是独一无二的，不可替代。UTEX向美国和世界各地的科学家、教育工作者和学生分发数千种活的藻类培养物。人们对藻类作为运输燃料、人类食物、动物饲料、药品和许多其他用途的来源的极大兴趣，使UTEX培养和服务成为研究人员必不可少的资源。每年，都有数百篇发表的研究文章提到UTEX菌株的使用，其中许多文章承认了NSF的支持。UTEX提供广泛的学生培训、管理藻类养殖的实践讲习班、内容丰富的互动网站(https://utex.org/)，)、物种鉴定服务、化学成分分析以及与从事藻类研究的学生广泛通信。UTEX人员在公立学校演讲，参观UTEX设施，协助教师和学生开展高中科学项目，并在当地的开放日活动上向数百名学童介绍藻类的科学重要性。UTEX是一个公共资源，其使命是促进、支持和使藻类能够用于研究、教育和实际应用。完成这一使命的UTEX的主要目标是：(1)维持一个精心策划的藻类生物多样性永久储存库，确保藻类研究的连续性和一致性；(2)以适度的成本向用户社区提供活的藻类培养和相关材料；(3)向研究人员、教育工作者和感兴趣的公众提供有关藻类的信息来源；以及(4)通过培训、支持服务和用品参与与用户社区的外联活动。该项目的其他目标是(1)通过改进培养维护技术和扩大低温储存中的菌株数量来增强UTEX保有物的安全性，(2)通过表征其基因组和代谢多样性来增加UTEX藻类菌株对用户社区的价值，(3)通过开发教材包和在线教学工具扩大与K-12和本科教育项目的互动，以及(4)加入并提供对理论和应用研究的新兴和扩展领域有价值的更多多样性的菌株。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Phototaxis in a wild isolate of the cyanobacterium Synechococcus elongatus

• DOI: 10.1073/pnas.1812871115
• 发表时间: 2018-12
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden

High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions

• DOI: 10.3390/biom9070276
• 发表时间: 2019-07-01
• 期刊: BIOMOLECULES
• 影响因子: 5.5
• 作者: Saad, Marwa Gamal;Dosoky, Noura Sayed;Shafik, Hesham Mohamed
• 通讯作者: Shafik, Hesham Mohamed

Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

• DOI: 10.1016/j.chom.2020.12.005
• 发表时间: 2021-02-10
• 期刊: CELL HOST & MICROBE
• 影响因子: 30.3
• 作者: Nelson，David R.;Hazzouri，Khaled M.;Salehi-Ashtiani，Kourosh
• 通讯作者: Salehi-Ashtiani，Kourosh

Extraction and Characterization of Lipids from Macroalgae

大型藻类脂质的提取和表征

• DOI: 10.1007/978-1-4939-9484-7_7
• 发表时间: 2019
• 期刊: Methods in molecular biology
• 作者: Nobles DR Jr, Manning SR

A droplet-based gradient microfluidic to monitor and evaluate the growth of Chlorella vulgaris under different levels of nitrogen and temperatures

• DOI: 10.1016/j.algal.2019.101657
• 发表时间: 2019-12-01
• 期刊: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
• 影响因子: 5.1
• 作者: Saad, Marwa Gamal;Selahi, Amirali;Shafik, Hesham Mohamed
• 通讯作者: Shafik, Hesham Mohamed