EAGER: Colorimetric Detection of Zika and other Viruses Through Their Surface Proteins and Nucleic Acids

EAGER：通过表面蛋白和核酸比色检测寨卡病毒和其他病毒

• 批准号: 1931100
• 负责人: Massood Tabib-Azar
• 金额: $ 18.63万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-05-15 至 2021-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1931100&HistoricalAwards=false
• 关键词: EAGER; Colorimetric; Detection; Zika; other

The investigator will develop field-deployable, inexpensive sensors to detect deadly and pathogenic viruses, in general, and Zika virus as a specific example to demonstrate feasibility. The proposed sensors will change their color when they detect specific viruses without any need for recurring DNA sequencing and amplification that requires time and are expensive for global deployment. Zika virus is expected to re-emerge this summer in Latin America and affect our cities such as Miami. The system can be adopted to detection of many other viruses such as Ebola, Crimean Congo hemorrhage fever, and Lassa fever. Polymerase chain reaction kits are currently available to detect the ZIKV virus but are time consuming and cannot be deployed in rural areas. The investigator is proposing to developing an aptamer hydrogel with embedded complementary strands that attach to the surface proteins of the ZIKV and absorbs them through its porous cavities causing the hydrogel to undergo (100%) volume and (~30%) color change caused by its embedded 50 nm diameter gold nano-particles.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

研究人员将开发可现场部署的廉价传感器，以检测致命和致病病毒，并将寨卡病毒作为一个具体的例子来证明可行性。拟议中的传感器在检测特定病毒时会改变颜色，而不需要重复进行DNA测序和扩增，这需要时间，而且全球部署成本高昂。寨卡病毒预计将于今年夏天在拉丁美洲重新出现，并影响我们的城市，如迈阿密。该系统还可用于埃博拉病毒、克里米亚刚果出血热、拉沙热等多种病毒的检测。聚合酶链反应试剂盒目前可用于检测ZIKV病毒，但耗时且无法在农村地区部署。研究者提出开发具有嵌入的互补链的适体水凝胶，所述互补链附着于ZIKV的表面蛋白并通过其多孔腔吸收它们，导致水凝胶经历由其嵌入的50 nm直径的金纳米粒子引起的（100%）体积和（~30%）颜色变化。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。