New peptide ligation technology for the rapid assembly of modified proteins

用于快速组装修饰蛋白的新肽连接技术

• 批准号: DP160101324
• 负责人: Prof Richard Payne
• 金额: $ 31.72万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-05 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160101324
• 关键词: New; peptide; ligation; technology; rapid

The project aims to develop novel technologies to enable the synthesis of modified proteins that are of widespread biological and therapeutic interest. More than 70 per cent of all human proteins are modified with a range of functionalities after translation from the ribosome. Although these modifications are of crucial importance for biological activity, characterising the effect of a given modification on function is difficult due to problems in obtaining the protein in pure form. The goal of this project is to develop a peptide ligation methodology to access pure modified proteins in a rapid manner through the exploitation of a new reaction recently discovered in our laboratory. The project plans to explore the scope and mechanism of the new reaction as well as its application in the total chemical synthesis and structure-function studies of important modified proteins.

该项目旨在开发新技术，以合成具有广泛生物学和治疗意义的修饰蛋白质。超过70%的人类蛋白质在从核糖体翻译后被修饰为具有一系列功能。虽然这些修饰对于生物活性至关重要，但由于获得纯形式的蛋白质存在问题，因此难以表征给定修饰对功能的影响。本项目的目标是开发一种肽连接方法，通过利用我们实验室最近发现的一种新反应，以快速的方式获得纯的修饰蛋白。该项目计划探索新反应的范围和机理，以及其在重要修饰蛋白质的全化学合成和结构-功能研究中的应用。