COLLABORATIVE RESEARCH: THE CRITICAL IMPORTANCE OF DIVERSE LEAF "HAIRSTYLES": INTEGRATIVE QUANTIFICATION OF ANATOMY, FUNCTION, EVOLUTION AND ECOLOGY OF TRICHOMES

合作研究：多样化叶子“发型”的至关重要性：毛状体的解剖学、功能、进化和生态学的综合量化

• 批准号: 1951244
• 负责人: Lawren Sack
• 金额: $ 68.99万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1951244&HistoricalAwards=false
• 关键词: COLLABORATIVE; RESEARCH; CRITICAL; IMPORTANCE; DIVERSE

Humankind depends on domesticated and wild plants for food, fiber, fuel, and fresh air. Understanding how plants work under current and future climates is therefore critical to human welfare. Plant leaf hairs (trichomes) are among the most complex, ubiquitous, important, and little understood biological traits. Leaf trichomes are present in more than half of plant species, have diverse structure and arrangements, and influence the environmental tolerances and productivity of both crop and non-crop species. The effects of trichomes include reflecting excessive light, preventing accumulation of surface water, reducing infection by pathogens, enhancing water capture from saturated air, reducing water loss to dry air, and fending off herbivores. Yet, leaf trichomes have been studied in detail for very few species, and therefore have been typically ignored in predictions of plant production, evolution and ecology. This study will refine and develop state-of-the-art methods to micro-image trichomes in diverse species, and use experiments and models to determine how their costs and benefits affect whole-plant performance. Additionally, the project team will analyze how trichomes vary across environments and through evolution for species of grapevine, for the model species Arabidopsis thaliana, and for entire floras of California and Hawaii. The project will train graduate students and post-doctoral scientists in state of the art technology and approaches to plant research, and will integrate general education, high school and undergraduate education with public dissemination of research, thereby increasing public awareness and fascination with plant structure and function. Through its collaborative research team, this project applies an innovative synthetic approach combining expertise in mathematics, physics, physiology, ecology and evolution. By integrating greenhouse and lab experimentation with state of the art 3D micro-imaging, herbarium work, experimental measurements, mechanistic and statistical modeling and phylogenetic analyses the research will quantify the anatomical diversity of leaf trichomes, their contributions to light absorption, water relations (wettability and foliar water uptake), gas exchange, and protection from herbivory, and their ecological and evolutionary associations. The first aim of this project is to combine cutting edge micro-visualization, experimentation and modeling to quantify the structure and multiple functions of leaf hairs in diverse species from at least 17 genera of flowering plants. The second aim is to integrate these multiple streams of data on the diversity of leaf trichomes and its influence on five known functions to determine how the net benefits scale up to influence whole plant performance. Third, an even wider perspective is necessary to determine how leaf trichomes evolved with climate within lineages and across floras. The project will leverage available data and herbarium material to focus on test cases of large-scale evolutionary and ecological patterns in leaf trichomes. The project’s Broader Impacts will provide curricula based on novel results via the UCLA Botanical Garden, and online modules including 3D models that can be accessed online and 3D printed. All data will be deposited in a publicly available database.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

人类依靠驯化和野生植物获取食物、纤维、燃料和新鲜空气。因此，了解植物在当前和未来气候下的工作方式对人类福祉至关重要。植物叶毛（毛状体）是最复杂，最普遍，最重要，但了解甚少的生物学性状。叶毛存在于超过一半的植物物种中，具有不同的结构和排列，并影响作物和非作物物种的环境耐受性和生产力。毛状体的作用包括反射过多的光线，防止地表水的积累，减少病原体的感染，增强饱和空气中的水分捕获，减少水分流失到干燥空气中，以及抵御食草动物。然而，叶毛状体已被详细研究了极少数物种，因此通常被忽略在植物生产，进化和生态学的预测。这项研究将改进和开发最先进的方法来对不同物种的毛状体进行显微成像，并使用实验和模型来确定其成本和效益如何影响整株植物的性能。此外，该项目小组将分析毛状体如何在不同环境中变化，并通过葡萄藤的进化，模式物种拟南芥，以及整个加州和夏威夷的植物群。该项目将培训研究生和博士后科学家掌握植物研究的最新技术和方法，并将普通教育、高中和本科教育与研究的公共传播相结合，从而提高公众对植物结构和功能的认识和兴趣。通过其合作研究团队，该项目应用了一种创新的综合方法，结合了数学、物理、生理学、生态学和进化学的专业知识。 通过将温室和实验室实验与最先进的3D显微成像，植物标本馆工作，实验测量，机械和统计建模以及系统发育分析相结合，该研究将量化叶毛的解剖多样性，它们对光吸收的贡献，水关系（润湿性和叶面吸水），气体交换和保护免受草食动物，以及它们的生态和进化关联。该项目的第一个目标是结合联合收割机尖端显微可视化，实验和建模，以量化来自至少17个属的开花植物的不同物种的叶毛的结构和多种功能。第二个目标是整合这些关于叶毛多样性及其对五种已知功能的影响的多个数据流，以确定净效益如何扩大到影响整个植物的性能。第三，一个更广泛的视角是必要的，以确定如何叶毛状体进化与气候内谱系和跨植物区系。该项目将利用现有的数据和植物标本材料，重点放在叶毛状体的大规模进化和生态模式的测试案例。该项目的“更广泛的影响”将通过加州大学洛杉矶分校植物园提供基于新成果的课程，以及在线模块，包括可以在线访问和3D打印的3D模型。所有数据将被保存在一个公开的数据库中。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估。

项目成果

Importance of the legacy effect for assessing spatiotemporal correspondence between interannual tree-ring width and remote sensing products in the Sierra Nevada

遗留效应对于评估内华达山脉年际树木年轮宽度与遥感产品之间的时空对应关系的重要性

• DOI: 10.1016/j.rse.2021.112635
• 发表时间: 2021
• 期刊: Remote Sensing of Environment
• 影响因子: 13.5
• 作者: Wong, Christopher Y.S.;Young, Derek J.N.;Latimer, Andrew M.;Buckley, Thomas N.;Magney, Troy S.
• 通讯作者: Magney, Troy S.

Wide variation in the suboptimal distribution of photosynthetic capacity in relation to light across genotypes of wheat

不同基因型小麦的光合能力与光的次优分布存在很大差异

• DOI: 10.1093/aobpla/plaa039
• 发表时间: 2020
• 期刊: AoB PLANTS
• 影响因子: 2.9
• 作者: Salter, William T;Merchant, Andrew;Trethowan, Richard M;Richards, Richard A;Buckley, Thomas N
• 通讯作者: Buckley, Thomas N

Do leaf nitrogen resorption dynamics align with the slow‐fast continuum? A test at the intraspecific level

• DOI: 10.1111/1365-2435.14029
• 发表时间: 2022-03
• 期刊: Functional Ecology
• 影响因子: 5.2
• 作者: Kevin Sartori;C. Violle;D. Vile;François Vasseur;P. Villemereuil;Justine Bresson;Lauren M. Gillespie;Leila R. Fletcher;L. Sack;E. Kazakou

Harvesting water from unsaturated atmospheres: deliquescence of salt secreted onto leaf surfaces drives reverse sap flow in a dominant arid climate mangrove, Avicennia marina

• DOI: 10.1111/nph.17461
• 发表时间: 2021-06-12
• 期刊: NEW PHYTOLOGIST
• 影响因子: 9.4
• 作者: Coopman, Rafael E.;Nguyen, Hoa T.;Ball, Marilyn C.
• 通讯作者: Ball, Marilyn C.

Shifting access to pools of shoot water sustains gas exchange and increases stem hydraulic safety during seasonal atmospheric drought

• DOI: 10.1111/pce.14080
• 发表时间: 2021-05-25
• 期刊: PLANT CELL AND ENVIRONMENT
• 影响因子: 7.3
• 作者: Bryant, Callum;Fuenzalida, Tomas, I;Ball, Marilyn C.
• 通讯作者: Ball, Marilyn C.