Excellence in Research: Antibiotics in the rhizosphere and resistance mechanisms in plants

卓越研究：根际抗生素和植物抗性机制

• 批准号: 2000157
• 负责人: Mentewab Ayalew
• 金额: $ 30万
• 依托单位: Spelman College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2000157&HistoricalAwards=false
• 关键词: Excellence; Research; Antibiotics; rhizosphere; resistance

The goal of this project is to investigate the relationship between metal uptake and antibiotic resistance in plants. Plants mine the soil to extract minerals and in so doing, may take up antibiotics produced by soil microorganisms that adversely affect normal plant function. How plants maintain adequate nutrition while limiting the uptake of antibiotics is unknown. We specifically investigate metal uptake in the model plant Arabidopsis thaliana and its resistance to the antibiotic kanamycin. We propose a model in which the interplay among various transporters explains the trade-offs between metal uptake and antibiotic resistance. Our approach that combines mathematical modeling and experimental validation would allow us to examine these otherwise complex interactions that are not intuitive. Moreover the project provides an excellent opportunity for interdisciplinary training of undergraduate students at Spelman College, a Historically Black College for women. Overall, the project will contribute to enhancing the educational environment through research, curriculum and student development opportunities.Fe and Zn are among the most abundant micronutrients found in plants. Their uptake and movement in the plant is highly regulated. Our preliminary results showed a link between metal uptake in plants and antibiotic resistance. Most notably, Fe uptake declined in plants exposed to kanamycin. We propose a model that integrates what is known about Fe and Zn homeostasis with the effects of the antibiotic kanamycin. Besides Fe, Zn and kanamycin, other factors taken into consideration are the main chelators of Fe and Zn, citrate and nicotianamine (NA), and their known or assumed transporters. The model is centered on xylem loading, a critical step prior for the long distance transport of nutrients. A corresponding dynamic mathematical model based on a Generalized Mass Action system will be formulated and tested both computationally and biochemically. It is expected that this model will offer a new paradigm for understanding the link between metal nutrition and antibiotics in Arabidopsis and possibly crop species.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

本项目的目标是研究植物对金属的吸收与抗生素抗性之间的关系。植物开采土壤以提取矿物质，这样做可能会吸收土壤微生物产生的抗生素，对正常植物功能产生不利影响。植物如何保持足够的营养，同时限制抗生素的吸收是未知的。我们专门研究金属吸收的模式植物拟南芥和它的抗性抗生素卡那霉素。我们提出了一个模型，其中各种转运蛋白之间的相互作用解释了金属吸收和抗生素耐药性之间的权衡。我们的方法结合了数学建模和实验验证，使我们能够检查这些复杂的相互作用，否则是不直观的。此外，该项目为Spelman学院（一所历史上的黑人女子学院）的本科生提供了一个跨学科培训的绝佳机会。总的来说，该项目将有助于通过研究，课程和学生发展机会来改善教育环境。铁和锌是植物中最丰富的微量营养素之一。它们在植物中的吸收和移动受到高度调节。我们的初步结果表明，植物的金属吸收和抗生素抗性之间存在联系。最值得注意的是，铁吸收下降，在植物暴露于卡那霉素。我们提出了一个模型，整合了已知的铁和锌的稳态与抗生素卡那霉素的影响。除了Fe、Zn和卡那霉素之外，考虑的其他因素是Fe和Zn的主要螯合剂、柠檬酸盐和烟酰胺（NA），以及它们已知或假定的转运体。该模型以木质部装载为中心，木质部装载是营养物质长距离运输的关键步骤。一个相应的动态数学模型的基础上广义质量作用系统将制定和测试计算和生化。 预计该模型将为了解拟南芥和可能的农作物物种中金属营养与抗生素之间的联系提供新的范式。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响进行评估而被认为值得支持。审查标准。