RUI: Mechanism of Antibiotic Resistance Associated with the Arabidopsis ABC Protein, Atwbc19

RUI：与拟南芥 ABC 蛋白 Atwbc19 相关的抗生素耐药性机制

• 批准号: 1052172
• 负责人: Mentewab Ayalew
• 金额: $ 37.07万
• 依托单位: Spelman College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1052172&HistoricalAwards=false
• 关键词: RUI; Mechanism; Antibiotic; Resistance; Associated

INTELLECTUAL MERIT ABC (ATP binding cassette ) proteins represent a gene family that has greatly expanded in plants, with twice as many members in Arabidopsis and rice (over 120) as any other sequenced genome of a multicellular organism (eg. human, worm, fly). The reason behind the expansion of this family in plants is unknown but hints to kingdom specific evolutionary constraints. This project focuses on the Arabidopsis ABC protein, Atwbc19 (ABCG19), a member of a plant-specific clade. Atwbc19 confers antibiotic resistance but the underlying mechanism is not clear. Preliminary results suggest that Atwbc19 does not function as a transporter but as a translational regulator. Thus the goal of this project is to demonstrate cleavage of Atwbc19 resulting in a soluble ABC protein, confirm its association with the translation apparatus and identify regulated transcripts. Ultimately, a new paradigm for antibiotic resistance in plants may be established.BROADER IMPACT The project has three educational objectives. First, long term research opportunities will be provided to undergraduates. Second, a research based course will be developed. And finally, research experiences will be provided in the traditional classroom through an upper level Plant Biology course. Overall, the project will contribute to the strategic goal of the College to increase student participation in research or internships from 30% to 100% by 2015. At the departmental level, the project will contribute to a curriculum that is being significantly revised towards a stronger emphasis on linking research to teaching. Ultimately, one goal is to inspire African American female students to engage in intellectual and personal development that leads them to Graduate School.

智力优势ABC(三磷酸腺苷结合盒)蛋白代表了一个在植物中得到极大扩展的基因家族，在拟南芥和水稻中的成员数量是任何其他多细胞有机体测序基因组的两倍(超过120个)。人、虫子、苍蝇)。这个家族在植物中的扩张背后的原因尚不清楚，但暗示着王国特有的进化限制。这个项目的重点是拟南芥ABC蛋白，Atwbc19(ABCG19)，一个植物特有的分支的成员。Atwbc19具有抗生素耐药性，但其潜在机制尚不清楚。初步结果表明，Atwbc19不是作为转运蛋白发挥作用，而是作为翻译调节因子发挥作用。因此，本项目的目标是证明Atwbc19的切割导致可溶的ABC蛋白，确认其与翻译装置的关联，并识别受调控的转录本。最终，可能会建立一种植物中抗生素耐药性的新范例。首先，为本科生提供长期的研究机会。第二，将开发一门以研究为基础的课程。最后，通过高级植物生物学课程，在传统课堂上提供研究经验。总体而言，该项目将有助于学院的战略目标，即到2015年将学生参与研究或实习的比例从30%提高到100%。在部门一级，该项目将促进正在大幅修订的课程，以便更加强调研究与教学的联系。归根结底，一个目标是激励非裔美国女学生参与智力和个人发展，从而引导她们进入研究生院。