GOALI: Innovative Interfaces for Two-Dimensional Liquid Chromatography

GOALI：二维液相色谱的创新接口

• 批准号: 2003734
• 负责人: Dwight Stoll
• 金额: $ 37.5万
• 依托单位: Gustavus Adolphus College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2003734&HistoricalAwards=false
• 关键词: GOALI; Innovative; Interfaces; Two; Dimensional

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, and in collaboration with Tom van de Goor of Agilent Technologies, Professor Dwight Stoll and his group at Gustavus Adolphus College are working to improve the capabilities of two-dimensional liquid chromatography (2D-LC) - an important approach to separating the components of complex chemical mixtures as part of the process of identifying and quantifying components. This academic/industrial collaboration seeks deeper understanding of the role of hardware interfaces in limiting the performance of current 2D-LC approaches. The research also seeks to develop innovative interfaces that will improve the speed and other performance metrics of analyses based on 2D-LC, while decreasing their cost. These innovations may be useful to scientists and engineers facing the need for detailed analysis of complex materials such as new protein-based drugs, chemical products based on renewable starting materials, and biofluids (including blood and urine) for which better analyses can enhance the understanding and diagnosis of disease. Dr. Stoll involves students at the high school and undergraduate levels in this cutting-edge research to prepare them to become the workforce of the future. He also develops simulations to facilitate learning about liquid chromatography and the more effective use of the technique across many sectors of the U.S. economy.In the majority of practical 2D-LC methods, the two separations are coupled using a valve-based interface that transfers small fractions of material exiting the first column to the inlet of the second column for further separation. Despite its critical role in the effectiveness of 2D-LC separations, innovation in the area of interface technology has been sluggish. The valves in common use today are conceptually quite similar to those used for the first 2D-LC separations four decades ago, and have weaknesses that negatively impact the performance of 2D-LC with respect to reliability, ease-of-use, and separation power. In this project, the Stoll Group is evaluating several innovative interface designs that either eliminate the valve entirely, or redefine the function of the valve in ways that have the potential to change the interface from a point of weakness to a point of strength of 2D-LC technology. As part of these studies they are evaluating the impact of different interface designs on other aspects of the system, such as the lifetime of second dimension columns. Finally, they are building simulation tools that recognize the unique features of the new interfaces to support facile development of new 2D-LC methods that are suitable for addressing contemporary separation challenges in the pharmaceutical, chemical, and life sciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学部化学测量和成像项目的支持下，并与安捷伦科技公司的Tom货车de古尔合作，Gustavus Adolphus学院的德怀特斯托尔教授和他的团队正在努力提高二维液相色谱（2D-LC）的能力，作为识别和量化组分过程的一部分，分离复杂化学混合物组分的重要方法。 这种学术/工业合作旨在更深入地了解硬件接口在限制当前2D-LC方法性能方面的作用。 该研究还寻求开发创新的接口，以提高基于2D-LC的分析的速度和其他性能指标，同时降低成本。这些创新可能对需要详细分析复杂材料的科学家和工程师有用，例如新的蛋白质药物，基于可再生原料的化学产品和生物流体（包括血液和尿液），更好的分析可以增强对疾病的理解和诊断。 Stoll博士让高中和本科生参与这项前沿研究，为他们成为未来的劳动力做好准备。 他还开发了模拟，以促进对液相色谱的学习，并在美国经济的许多部门更有效地使用该技术。在大多数实用的2D-LC方法中，两种分离使用基于阀门的接口耦合，该接口将离开第一柱的小部分材料转移到第二柱的入口进行进一步分离。尽管其在2D-LC分离的有效性方面发挥着关键作用，但接口技术领域的创新一直停滞不前。目前常用的阀在概念上与四十年前用于第一次2D-LC分离的阀非常相似，并且具有在可靠性、易用性和分离能力方面对2D-LC的性能产生负面影响的弱点。在这个项目中，Stoll集团正在评估几种创新的接口设计，这些设计要么完全消除阀门，要么重新定义阀门的功能，从而有可能将接口从2D-LC技术的弱点转变为优势。作为这些研究的一部分，他们正在评估不同界面设计对系统其他方面的影响，例如二维柱的寿命。最后，他们正在构建能够识别新界面独特功能的模拟工具，以支持新的2D-LC方法的简便开发，这些方法适用于解决制药、化学和生命科学领域的当代分离挑战。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

An approach to high throughput measurement of accurate retention data in liquid chromatography

液相色谱中准确保留数据的高通量测量方法

• DOI: 10.1016/j.chroma.2022.463350
• 发表时间: 2022
• 期刊: Journal of Chromatography A
• 影响因子: 4.1
• 作者: Stoll, Dwight R.;Kainz, Gudrun;Dahlseid, Tina A.;Kempen, Trevor J.;Brau, Tyler;Pirok, Bob W.J.
• 通讯作者: Pirok, Bob W.J.

Modelling of analyte profiles and band broadening generated by interface loops used in multi-dimensional liquid chromatography

多维液相色谱中使用的界面环产生的分析物谱和谱带展宽的建模

• DOI: 10.1016/j.chroma.2021.462578
• 发表时间: 2021
• 期刊: Journal of Chromatography A
• 影响因子: 4.1
• 作者: Moussa, Ali;Lauer, Thomas;Stoll, Dwight;Desmet, Gert;Broeckhoven, Ken
• 通讯作者: Broeckhoven, Ken

Implications of dispersion in connecting capillaries for separation systems involving post-column flow splitting

涉及柱后分流的分离系统连接毛细管中分散的影响

• DOI: 10.1016/j.chroma.2021.461893
• 发表时间: 2021
• 期刊: Journal of Chromatography A
• 影响因子: 4.1
• 作者: Gunnarson, Caden;Lauer, Thomas;Willenbring, Harrison;Larson, Eli;Dittmann, Monika;Broeckhoven, Ken;Stoll, Dwight R.
• 通讯作者: Stoll, Dwight R.

Characterization of a high throughput approach for large scale retention measurement in liquid chromatography

液相色谱中大规模保留测量的高通量方法的表征

• DOI: 10.1016/j.chroma.2023.464182
• 发表时间: 2023
• 期刊: Journal of Chromatography A
• 影响因子: 4.1
• 作者: Kempen, Trevor;Dahlseid, Tina;Lauer, Thomas;Florea, Alexandru C.;Aase, Isabella;Cole-Dai, Nathan;Kaur, Simerjit;Southworth, Caroline;Grube, Kathleen;Bhandari, Jos
• 通讯作者: Bhandari, Jos

Accuracy of retention model parameters obtained from retention data in liquid chromatography

从液相色谱保留数据获得的保留模型参数的准确性

• DOI: 10.1002/jssc.202100911
• 发表时间: 2022
• 期刊: Journal of Separation Science
• 影响因子: 3.1
• 作者: Brau, Tyler;Pirok, Bob;Rutan, Sarah;Stoll, Dwight
• 通讯作者: Stoll, Dwight