BBSRC-NSF/BIO: A holistic approach to understand drought adaptation in plants, their symbionts, and free-living microbiomes
BBSRC-NSF/BIO:了解植物、共生体和自由生活微生物组的干旱适应的整体方法
基本信息
- 批准号:2016351
- 负责人:
- 金额:$ 71.92万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-06-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
For decades, it has been clear that soil microbes strongly affect plant health. For this reason, plant microbiomes—which mostly come from soil—have great potential to help improve agricultural productivity and restore damaged ecosystems. Because microbiomes are so complex, it is unclear how and why they affect plants. Part of the answer may lie in the evolutionary histories of plants and their microbiomes, which share a habitat and therefore must adapt to the same environmental stresses, such as drought. The research funded by this award will test the hypotheses that (1) drought-adapted soil microbiomes help plants tolerate drought, and (2) drought-adapted plants help soil microbes survive drought. DNA sequencing will be used to identify differences in drought-adapted versus non-adapted soil microbiomes. Then, physiological and biochemical techniques, such as CT-scanning of root structures, will reveal the effects of those drought-adapted and non-adapted microbiomes on plant health. These experiments will be done using both corn and Eastern Gamagrass, a native species. This research has the potential to improve current methods for predicting the effects of soil microbes on plant health, and thus impact agriculture and conservation practices. This award will also grow the American scientific workforce by supporting the training of one post-doctoral researcher, one Ph.D. student researcher, and two undergraduate student researchers. Finally, this award supports a portable museum exhibit that brings scientific content to hundreds of people in rural communities across Kansas. This project is motivated by the lack of knowledge of how adaptation to a shared environmental stressor affects plant-microbiome interactions. Because root microbiomes are derived from soil, the constituent microbial lineages encounter environmental stressors both with and without a host. Whether the same microbial genes and traits contribute to stress tolerance and survival in both situations, or whether there is a fitness tradeoff between host-associated vs. free-living stages is not clear. Similarly, it is unknown whether microbiome adaptation to a given environmental challenge impacts the ability of a plant host to withstand the same challenge. To address these questions, drought will be used as a model stressor. First, shotgun metagenome sequencing of soils from across two natural precipitation gradients will characterize how historical water limitation shapes community-wide gene content, after controlling for variation in soil structure and chemical content measured using 3D X-ray CT and ICP-MS, respectively. Second, repeated metagenome and metatranscriptome sequencing will test for changes in the frequency and expression of microbial genes in natural soils after six months of experimentally induced drought. This experiment will be replicated in mesocosms both with and without plant hosts. Finally, the effects of drought-adapted and non-adapted soil microbiomes on plant physiology and drought tolerance will be measured using growth assays, RNA-seq, ionomics, and laser ablation tomography. This research may disentangle the genetic, physiological, and ecological interdependencies that shape the evolution of plant microbiomes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
几十年来,人们已经清楚,土壤微生物强烈影响植物健康。因此,植物微生物群(主要来自土壤)在帮助提高农业生产力和恢复受损生态系统方面具有巨大潜力。由于微生物组是如此复杂,目前还不清楚它们如何以及为什么影响植物。部分答案可能在于植物及其微生物组的进化历史,它们共享一个栖息地,因此必须适应相同的环境压力,例如干旱。该奖项资助的研究将测试以下假设:(1)适应干旱的土壤微生物组帮助植物耐受干旱,(2)适应干旱的植物帮助土壤微生物在干旱中生存。DNA测序将用于确定干旱适应与非适应土壤微生物的差异。然后,生理和生化技术,如根结构的CT扫描,将揭示这些干旱适应和非适应微生物对植物健康的影响。这些实验将使用玉米和当地物种Eastern Gamagrass进行。这项研究有可能改善目前预测土壤微生物对植物健康影响的方法,从而影响农业和保护实践。该奖项还将通过支持一名博士后研究人员的培训,一名博士,学生研究员和两名本科生研究员。最后,该奖项支持一个便携式博物馆展览,为堪萨斯农村社区的数百人带来科学内容。该项目的动机是缺乏对共同环境压力的适应如何影响植物-微生物组相互作用的知识。由于根微生物组来源于土壤,因此组成微生物谱系在有宿主和无宿主的情况下都会遇到环境压力。在这两种情况下,是否相同的微生物基因和特征有助于胁迫耐受性和生存,或者宿主相关阶段与自由生活阶段之间是否存在适应性权衡,目前尚不清楚。同样,微生物组对特定环境挑战的适应是否会影响植物宿主承受相同挑战的能力也是未知的。 为了解决这些问题,干旱将被用作一个模型的压力。首先,在控制分别使用3D X射线CT和ICP-MS测量的土壤结构和化学含量的变化后,对两个自然降水梯度的土壤进行鸟枪宏基因组测序,将表征历史水分限制如何影响整个社区的基因含量。第二,重复的宏基因组和元转录组测序将测试在实验诱导干旱六个月后自然土壤中微生物基因的频率和表达的变化。该实验将在有和没有植物宿主的围隔生态系统中重复进行。最后,干旱适应和非适应土壤微生物组对植物生理和耐旱性的影响将使用生长测定,RNA-seq,ionomics和激光消融断层扫描来测量。这项研究可能会解开遗传,生理和生态的相互依赖性,塑造植物微生物组的演变。这个奖项反映了NSF的法定使命,并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Microbial effects on plant phenology and fitness
微生物对植物物候和适应性的影响
- DOI:10.1002/ajb2.1743
- 发表时间:2021
- 期刊:
- 影响因子:3
- 作者:O'Brien, Anna M.;Ginnan, Nichole A.;Rebolleda‐Gómez, María;Wagner, Maggie R.
- 通讯作者:Wagner, Maggie R.
Utility of large subunit for environmental sequencing of arbuscular mycorrhizal fungi: a new reference database and pipeline
- DOI:10.1111/nph.17080
- 发表时间:2020-12-04
- 期刊:
- 影响因子:9.4
- 作者:Delavaux, Camille S.;Sturmer, Sidney L.;Bever, James D.
- 通讯作者:Bever, James D.
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Maggie Wagner其他文献
Maggie Wagner的其他文献
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{{ truncateString('Maggie Wagner', 18)}}的其他基金
RESEARCH-PGR: Genetics and Mechanisms of Microbe-dependent Heterosis
研究-PGR:微生物依赖性杂种优势的遗传学和机制
- 批准号:
2033621 - 财政年份:2021
- 资助金额:
$ 71.92万 - 项目类别:
Standard Grant
NSF Postdoctoral Fellowship in Biology FY 2016
2016 财年 NSF 生物学博士后奖学金
- 批准号:
1612951 - 财政年份:2016
- 资助金额:
$ 71.92万 - 项目类别:
Fellowship Award
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