I-Corps: Interferometric Imaging for Multiplexed Molecular Kinetics

I-Corps：多重分子动力学的干涉成像

• 批准号: 2027109
• 负责人: Selim Unlu
• 金额: $ 5万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2021-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027109&HistoricalAwards=false
• 关键词: Corps; Interferometric; Imaging; Multiplexed; Molecular

The broader impact/commercial potential of this I-Corps project is to explore translation of a sensor for better diagnostics and therapeutics. The proposed technology can be created at low cost, enabling an instrument that integrates parallel sensors for a high-throughput system.This I-Corps project explores applications for the Interferometric Reflectance Imaging Sensor (IRIS), a label-free detection (LFD) system. The proposed system uses optical biosensors in multiplexed imaging detection of molecular binding kinetics on Si-based microfluidic chips as low-cost consumables. Hundreds of capture probes can be arrayed on the IRIS chip and tested against a target molecule in parallel. The proposed system addresses needs for multiplexed and robust small molecule affinity measurements, which can enable the discovery of new ligands for detection and monitoring of small molecules for compact sensors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该I-Corps项目的更广泛的影响/商业潜力是探索传感器的翻译，以获得更好的诊断和治疗学。提出的技术可以以低成本的形式创建，从而使能够集成高通量系统的平行传感器的仪器。本I-Corps项目探讨了干涉反射成像传感器（IRIS）的应用，这是一个无标签检测（LFD）系统。拟议的系统在基于Si的微流体芯片上的分子结合动力学的多路形成像检测中使用光学生物传感器作为低成本消耗品。数百个捕获探针可以在虹膜芯片上排列，并并行针对目标分子进行测试。所提出的系统解决了对多重和稳健的小分子亲和力测量的需求，这可以使发现新的配体进行检测和监测小分子以进行紧凑的传感器。该奖项反映了NSF的法定任务，并通过使用该基金会的知识分子优点和广泛影响来评估NSF的法定任务，并被视为值得的支持。

项目成果

Simultaneous evaluation of multiple microarray surface chemistries through real-time interferometric imaging

• DOI: 10.1007/s00216-019-02276-1
• 发表时间: 2020-01-04
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Chiodi, Elisa;Sola, Laura;Chiari, Marcella
• 通讯作者: Chiari, Marcella