I-Corps: Interferometric Imaging for Multiplexed Molecular Kinetics

I-Corps：多重分子动力学的干涉成像

• 批准号: 2027109
• 负责人: Selim Unlu
• 金额: $ 5万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2021-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027109&HistoricalAwards=false
• 关键词: Corps; Interferometric; Imaging; Multiplexed; Molecular

The broader impact/commercial potential of this I-Corps project is to explore translation of a sensor for better diagnostics and therapeutics. The proposed technology can be created at low cost, enabling an instrument that integrates parallel sensors for a high-throughput system.This I-Corps project explores applications for the Interferometric Reflectance Imaging Sensor (IRIS), a label-free detection (LFD) system. The proposed system uses optical biosensors in multiplexed imaging detection of molecular binding kinetics on Si-based microfluidic chips as low-cost consumables. Hundreds of capture probes can be arrayed on the IRIS chip and tested against a target molecule in parallel. The proposed system addresses needs for multiplexed and robust small molecule affinity measurements, which can enable the discovery of new ligands for detection and monitoring of small molecules for compact sensors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

I-Corps项目更广泛的影响/商业潜力是探索传感器的翻译，以更好地诊断和治疗。所提出的技术可以以低成本创建，使仪器集成了高通量系统的并行传感器。这个I-Corps项目探索了干涉反射成像传感器（IRIS）的应用，这是一种无标签检测（LFD）系统。该系统采用光学生物传感器对硅基微流控芯片上的分子结合动力学进行多路成像检测，作为低成本耗材。IRIS芯片上可以排列数百个捕获探针，并对目标分子进行并行测试。提出的系统解决了多路和鲁棒小分子亲和测量的需求，这可以发现用于检测和监测小型传感器小分子的新配体。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Simultaneous evaluation of multiple microarray surface chemistries through real-time interferometric imaging

• DOI: 10.1007/s00216-019-02276-1
• 发表时间: 2020-01-04
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Chiodi, Elisa;Sola, Laura;Chiari, Marcella
• 通讯作者: Chiari, Marcella