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PFI-TT: Interferometric Imaging for Multiplexed Molecular Kinetics

PFI-TT：多重分子动力学干涉成像

基本信息

批准号：
1941195
负责人：
Selim Unlu
金额：
$ 25万
依托单位：
Trustees of Boston University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-01 至 2022-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1941195&HistoricalAwards=false
关键词：
PFI TT Interferometric Imaging Multiplexed

项目摘要

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project is to advance the current state-of-the-art biological detection technologies. Both in vitro diagnostics and the pharmaceutical industry rely on biotechnology tools to discover and develop new molecular interactions. Since all biological pathways rely on protein-DNA and protein-protein interactions, not only is accurate characterization of these interactions crucial, but they are prime targets for disruption with small molecules in disease treatment. Emerging diseases call for new diagnostic tests and thus new molecular discoveries. This Technology Translation (PFI-TT) project will pave the way to commercialization of a new technology based on light interference. There will be a direct societal impact by enabling better diagnostics and therapeutics. In addition, we will train several women engineering PhD students in technology development and entrepreneurship. The proposed project builds on innovations in Interferometric reflectance imaging sensor (IRIS) technology. The underlying measurement principle is based on the interference of light reflected from an optically transparent thin film—the same phenomenon that gives rainbow colors to a soap film when illuminated by white light. The proposed innovation uses a different approach than the conventional method of enhancing the signal through complex optical resonances; instead, we exploit the power of signal averaging to achieve very high sensitivity in a simple instrument platform. By design, the IRIS technique is resilient to the background signal due to variations in the solution (termed “bulk effect”). The proposed new technique will further improve the ability to distinguish molecular binding on the surface from the variations in the bulk solution. The proposed IRIS platform is unique among all label-free optical biosensors with multiplexed detection of molecular binding kinetics on Si microfludic chips as low-cost consumables. Hundreds of capture probes can be arrayed on the IRIS chip and tested against a target molecule in parallel. The expected low cost of the instrument will allow for integration of parallel sensors to achieve high throughput and multiplexing simultaneously.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这一创新-技术转化伙伴关系(PFI-TT)项目的更广泛影响/商业潜力是促进当前最先进的生物检测技术。体外诊断和制药业都依赖生物技术工具来发现和开发新的分子相互作用。由于所有的生物途径都依赖于蛋白质-DNA和蛋白质-蛋白质的相互作用，因此不仅对这些相互作用的准确描述至关重要，而且它们也是疾病治疗中用小分子干扰的主要目标。新出现的疾病需要新的诊断测试，从而需要新的分子发现。这一技术转化(PFI-TT)项目将为一种基于光干涉的新技术的商业化铺平道路。通过实现更好的诊断和治疗，将产生直接的社会影响。此外，我们还将在技术开发和创业方面培训几名女性工程学博士生。拟议的项目建立在干涉反射成像传感器(IRIS)技术创新的基础上。基本的测量原理是基于光学透明薄膜反射的光的干涉--这与肥皂膜在白光照射下呈现彩虹颜色的现象相同。建议的创新使用了一种与传统方法不同的方法，即通过复杂的光学共振来增强信号；相反，我们利用信号平均的力量在简单的仪器平台上实现非常高的灵敏度。通过设计，IRIS技术对由于溶液变化而产生的背景信号具有弹性(称为“体效应”)。拟议的新技术将进一步提高区分表面分子结合和主体溶液中的变化的能力。提出的IRIS平台在所有无标记光学生物传感器中是独一无二的，它以低成本的消耗品对硅微流控芯片上的分子结合动力学进行多路检测。数百个捕获探针可以排列在IRIS芯片上，并与目标分子进行平行测试。该仪器的预期低成本将允许集成并行传感器，以同时实现高吞吐量和多路复用。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。