Collaborative Research: IIBR Instrumentation: The Nanosizer - A new tool for the preparation of arbitrary bioactive surfaces

合作研究：IIBR 仪器：Nanosizer - 用于制备任意生物活性表面的新工具

• 批准号: 2032176
• 负责人: Adam Braunschweig
• 金额: $ 45万
• 依托单位: Research Foundation CUNY - Advanced Science Research Center
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2032176&HistoricalAwards=false
• 关键词: Collaborative; Research; IIBR; Instrumentation; Nanosizer

An award is made to Northwestern University and the City University of New York to develop an instrument capable of synthesizing arbitrary 3D nanopatterns of biologically active materials, coined “the Nanosizer.” The development of said instrument, the instrument itself, and the new photochemistry developed, will have wide reaching impacts on trainees, the nearby Chicago and New York City communities, and the broader scientific community. Both Mirkin and Braunschweig groups have previously and will continue to mentor trainees, including undergraduates, graduate students, and postdoctoral fellows, on the design of experiments and communication skills. Importantly, the researchers will lead the experimentation effort and work closely with the PIs on the dissemination of results through high-impact publications, patents, grant reports, and conference presentations. Exposure to all aspects of the research process will provide trainees with the highest probability of success on their paths forward, as proven by the track record of academic and industrial job placement from the two groups. The neighboring Chicago and New York communities stand to benefit from the award, through participation in outreach endeavors. Braunschweig group will participate in the Research Internships in Materials Science and Mirkin members will volunteer with Science In your Community Center with the goal of engaging K-12 students, primarily those from traditionally underrepresented groups, in science and technology. The Nanosizer will make arbitrary 3D nanopatterns of biologically active macromolecules with 100 nm resolution a reality through in situ surface patterning and fully orthogonal biomacromolecule construction. Through a combination of beam-pen lithography with 100 nm pixel resolution, precise multiwavelength irradiation control, and continuous flow of variable reagent streams, the Nanosizer will enable control over the reaction conditions at any 100 nm pixel over a 1 cm2 area. This, in conjunction with the development of orthogonal photochemistries for surface functionalization and synthesis of biomolecules, such as oligopeptides, oligonucleotides, and glycans, will enable the massively parallel generation of biomolecule arrays, with each pixel containing a chemically unique and well-defined molecule. Synthetic interfaces coated with biologically active materials have screening and functional applications in nearly every field of biology. The Nanosizer will provide monumental improvements in batch-to-batch reproducibility, resolution size, feature density, feature quantity, and overall cost in the preparation of such substrates. Results from these studies will be published in peer-reviewed journals, presented at scientific meetings, broadly distributed through commercialization, and incorporated into outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

西北大学和纽约城市大学获得了一项奖励，以开发一种能够合成生物活性材料的任意3D纳米片的仪器，称为“纳米尺寸仪”。所述仪器的开发、仪器本身以及开发的新的光化学将对受训者、附近的芝加哥和纽约市社区以及更广泛的科学界产生广泛的影响。米尔金和布伦瑞克集团以前和将继续指导学员，包括本科生，研究生和博士后研究员，对实验设计和沟通技巧。重要的是，研究人员将领导实验工作，并与PI密切合作，通过高影响力的出版物，专利，赠款报告和会议演示来传播结果。接触研究过程的各个方面将为学员提供最大的成功概率，正如这两个群体的学术和工业就业记录所证明的那样。邻近的芝加哥和纽约社区将通过参与外展活动从该奖项中受益。布伦瑞克组将参加在材料科学和米尔金成员的研究实习将与科学志愿者在您的社区中心与从事K-12学生的目标，主要是那些从传统上代表性不足的群体，在科学和技术。Nanosizer将通过原位表面图案化和完全正交的生物大分子结构，实现具有100 nm分辨率的生物活性大分子的任意3D纳米图案。通过将具有100 nm像素分辨率的射束笔光刻、精确的多波长照射控制和可变试剂流的连续流动相结合，Nanosizer将能够在1 cm 2面积上的任何100 nm像素处控制反应条件。这与用于表面功能化和合成生物分子（如寡肽、寡核苷酸和聚糖）的正交光化学的发展相结合，将使得能够大规模并行生成生物分子阵列，其中每个像素包含化学上独特且明确定义的分子。涂覆有生物活性材料的合成界面在生物学的几乎每个领域都有筛选和功能应用。Nanosizer将在批次间重现性、分辨率大小、特征密度、特征数量和制备此类基底的总体成本方面提供巨大的改进。这些研究的结果将发表在同行评审的期刊上，在科学会议上发表，通过商业化广泛传播，并纳入外展演示文稿。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。

项目成果

Orthogonal Images Concealed Within a Responsive 6‐Dimensional Hypersurface

• DOI: 10.1002/adma.202100803
• 发表时间: 2021-04
• 期刊: Advanced Materials
• 影响因子: 29.4
• 作者: Yerzhan S. Zholdassov;Daniel J. Valles;Samiha Uddin;Joanna Korpanty;N. Gianneschi;Adam B. Braunschweig

Acceleration of Diels-Alder reactions by mechanical distortion

• DOI: 10.1126/science.adf5273
• 发表时间: 2023-06-08
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Zholdassov，Yerzhan S.;Yuan，Li;Braunschweig，Adam B.
• 通讯作者: Braunschweig，Adam B.

Evolution and applications of polymer brush hypersurface photolithography

• DOI: 10.1039/d1py01073e
• 发表时间: 2021-09
• 期刊: Polymer Chemistry
• 影响因子: 4.6
• 作者: Daniel J. Valles;Yerzhan S. Zholdassov;Adam B. Braunschweig

Glycopolymer Microarrays with Sub‐Femtomolar Avidity for Glycan Binding Proteins Prepared by Grafted‐To/Grafted‐From Photopolymerizations

通过 Grafted–To/Grafted–From 光聚合制备的糖聚合物微阵列，对聚糖结合蛋白具有亚飞摩尔亲和力

• DOI: 10.1002/anie.202105729
• 发表时间: 2021
• 期刊: Angewandte Chemie International Edition
• 作者: Valles, Daniel J.;Zholdassov, Yerzhan S.;Korpanty, Joanna;Uddin, Samiha;Naeem, Yasir;Mootoo, David R.;Gianneschi, Nathan C.;Braunschweig, Adam B.
• 通讯作者: Braunschweig, Adam B.