Calcium Phosphate Mineralization of Hydrogels, their Microstructure and Mechanical Behavior

水凝胶的磷酸钙矿化、微观结构和力学行为

• 批准号: 2035122
• 负责人: Rosa Espinosa-Marzal
• 金额: $ 42.04万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2035122&HistoricalAwards=false
• 关键词: Calcium; Phosphate; Mineralization; Hydrogels; their

This grant supports research that advances knowledge of the manufacturing process promoting both the progress of science and national health. Every year, over half a million patients need bone defect repairs in the U.S. and advanced manufacturing methods of materials for bone replacement and regeneration are needed. A promising method is the use of calcium phosphate mineralization of hydrogel materials. Calcium phosphate is the mineral component of bone and mineralized hydrogels could yield both suitable biomimetic properties and mechanical strength as a bone defect replacement material. Despite the significant advance in understanding mineralization, knowledge of the relation between the mineralization pathway, the microstructure and the mechanical response of these hybrid materials is still lacking, which hinders advances in manufacturing science. This grant supports fundamental research to provide that needed knowledge for the development of mineralization processes of hydrogels that allow control of their structure-mechanical property relation. The gained knowledge will be applicable to advance manufacturing of biomaterials via abiotic mineralization for bone replacement. Furthermore, the gained insight will be also relevant to control biotic mineralization pathways, and hence, for bone regeneration based on tissue engineering. Therefore, results from this research will benefit the U.S. society. This research involves several disciplines including materials science and engineering, chemistry, and mechanics and will contribute to the development of workforce in the U.S. The multi-disciplinary research will help broaden participation of underrepresented groups in research and positively impact engineering education.The overall objective of this research is to comprehend, quantify and model the relation between mineralization pathways of calcium phosphate in the presence of amorphous calcium carbonate (ACC) precursors, the microstructure of the mineralized hydrogel and its mechanical response. This grant supports the research that will elucidate i) how the mineralization pathway is affected by the presence of ACC in the hydrogels, ii) the relation between polymer properties (charge density, chemical crosslinks vs. physical entanglements and self-assembly capability), mineralization mechanisms and microstructure, iii) the effect of the solution chemistry on the mineralization rate, and iv) the relation between microstructure and rheology of mineralized hydrogels. The findings of this work will be used to develop a conceptual framework to predict strengthening mechanisms of hydrogels via calcium phosphate mineralization as a function of solution composition and hydrogel properties. Experiments are designed to examine specific underlying mechanisms and test the posed hypotheses concerning issues such as the initial formation of a liquid-phase precursor en route to mineralization, the induced polymer-mineral interaction strength, and the nanoparticle aggregation in the hydrogel.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该补助金支持研究，促进生产过程的知识，促进科学进步和国民健康。在美国，每年有超过50万患者需要进行骨缺损修复，因此需要先进的骨替代和再生材料制造方法。一种很有前途的方法是利用磷酸钙矿化水凝胶材料。磷酸钙是骨的矿物质成分，矿化水凝胶可以作为骨缺损的替代材料，具有合适的仿生性能和机械强度。尽管在理解矿化方面取得了重大进展，但仍然缺乏对这些混合材料的矿化途径、微观结构和机械响应之间关系的了解，这阻碍了制造科学的进步。该补助金支持基础研究，为水凝胶矿化过程的发展提供所需的知识，从而控制其结构-机械性能关系。所获得的知识将适用于通过非生物矿化用于骨替代的生物材料的先进制造。此外，所获得的洞察力也将与控制生物矿化途径有关，因此，基于组织工程的骨再生。因此，这项研究的结果将有利于美国社会。这项研究涉及多个学科，包括材料科学与工程，化学和力学，并将有助于在美国劳动力的发展。多学科的研究将有助于扩大在研究中的代表性不足的群体的参与，并积极影响工程教育。这项研究的总体目标是了解，量化和模拟在无定形碳酸钙（ACC）前体存在下磷酸钙的矿化途径、矿化水凝胶的微观结构及其机械响应之间的关系。该基金支持的研究将阐明i）水凝胶中ACC的存在如何影响矿化途径，ii）聚合物性质之间的关系，（电荷密度、化学交联与物理缠结和自组装能力）、矿化机制和微观结构，iii）溶液化学对矿化速率的影响，矿化水凝胶的微观结构与流变性之间的关系。这项工作的结果将被用来开发一个概念框架，通过磷酸钙矿化作为溶液组成和水凝胶性能的函数来预测水凝胶的强化机制。实验的目的是研究具体的潜在机制，并测试提出的假设有关的问题，如初始形成的液相前体途中矿化，诱导聚合物-矿物相互作用强度，该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查进行评估，被认为值得支持的搜索.

项目成果

Insight into the assembly of lipid-hyaluronan complexes in osteoarthritic conditions

深入了解骨关节炎条件下脂质-透明质酸复合物的组装

• DOI: 10.1116/6.0002502
• 发表时间: 2023
• 期刊: Biointerphases
• 影响因子: 2.1
• 作者: Sun, Kangdi;Shoaib, Tooba;Rutland, Mark W.;Beller, Joesph;Do, Changwoo;Espinosa-Marzal, Rosa M.
• 通讯作者: Espinosa-Marzal, Rosa M.