Collaborative Research: IODP-enabled Insights into Fungi and Their Metabolic Interactions with Other Microorganisms in Deep Subsurface Hydrothermal Sediments
合作研究:借助 IODP 深入了解深层地下热液沉积物中的真菌及其与其他微生物的代谢相互作用
基本信息
- 批准号:2048489
- 负责人:
- 金额:$ 3.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-15 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The marine subsurface is one of the least explored habitats on Earth. International Ocean Discovery Program (IODP) Expedition 385 drilled into the seafloor in Guaymas Basin, Mexico, and was the first to drill directly into subsurface sediments and sediment-hosted basalt sill intrusions of an active hydrothermal basin. This expedition provides a direct microbiological window into a deep hydrothermal biosphere across an active plate spreading center where complex hydrocarbons are generated by heating of buried organic matter under high temperature and pressure. Mounting evidence suggests that Fungi constitute an active and ecologically important fraction of the subsurface biosphere community. This is especially true in organic-rich continental margin sediments that are ideal for colonization by aerobic and anaerobic Fungi, where fungal activities may contribute significantly to nutrient cycling. Major knowledge gaps in our knowledge of subsurface microbiota preclude our ability to estimate their full impact: how active Fungi are distributed along temperature and depth gradients, the range of substrates utilized by active cells, and how Fungi may cooperate with bacteria in degradation of complex organic matter, including hydrocarbons. Fungi are known to participate in degradation of refractory organics and cycling of metals and to produce novel metabolites with interesting properties. This project informs us on origins of different lineages of microbial life on Earth, the extent of marine subsurface carbon cycling, limits of life, how life adapts to environmental change, and the potential for Fungi to accelerate the biodegradation of complex hydrocarbons. Given the extent of the potential subsurface biosphere, Fungi likely play an important role in global nutrient cycling. The culture collection of fungal isolates created by this project will be available for exploration of their ecology and novel properties by other interested researchers, and may also yield insights into basal fungal lineages. These biogeochemical and potential evolutionary outcomes are of great interest to other research disciplines, educators, and students alike. The project’s K-16 education program capitalizes on programs aimed at increasing involvement of under-represented undergraduate populations in research. High school students, undergraduates, a graduate student, and postdoc are involved in the research. An art-in-science project with a local high school is being displayed at the community library along with education materials on marine Fungi and their ecological roles. This project examines how abundance, diversity and distribution of Fungi and co-inhabiting bacteria and archaea changes in subsurface sediment samples exhibiting a wide range of in situ temperatures and pressure, what the active fraction of cells is along these gradients, and whether/how Fungi impact carbon cycling in this biosphere by interacting metabolically with bacteria to break down hydrocarbon substrates. The project is assessing the activities of in situ microorganisms in this active hydrothermal subsurface biosphere using a cutting-edge combination of molecular approaches and culture-based studies of enrichments and microbial isolates applied to an extensive collection of samples from 8 sites in Guaymas Basin varying in temperature profile, presence of old, buried magmatic sills, and geochemical conditions. The investigators are examining 1) marker genes and metagenomes of sorted active cells using new bioorthogonal non-canonical amino acid tagging (BONCAT) approaches, 2) the distribution of bacterial, archaeal, and fungal cells and their marker genes along depth and geochemical gradients using microscopy, ‘meta-omics’ and lipid biomarker analyses, 3) substrate usage by fungal isolates, 4) metabolite pools, nutrients, and hydrocarbons with depth, and 5) fungal metabolism of complex organics (and syntrophies between Fungi and Bacteria) using time-course stable isotope probing of RNA from culture-based studies coupled with analyses of expressed genes and pools of metabolites.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
海洋地下是地球上最少探索的栖息地之一。国际海洋发现计划第385号考察队在墨西哥瓜伊马斯盆地的海底钻探,是第一个直接钻探地下沉积物和活跃热液盆地沉积物托管玄武岩岩床侵入体的考察队。这次考察提供了一个直接的微生物窗口,进入一个活动板块扩张中心的深层热液生物圈,在那里,复杂的碳氢化合物是通过在高温高压下加热埋藏的有机物而产生的。越来越多的证据表明,真菌构成了地下生物圈群落的一个活跃的和生态上重要的部分。在富含有机物的大陆边缘沉积物中尤其如此,这些沉积物是好氧和厌氧真菌定植的理想场所,真菌活动可能对营养循环有重大贡献。我们对地下微生物群的认识存在重大知识缺口,因此无法估计它们的全部影响:活性真菌如何沿沿着温度和深度梯度分布,活性细胞利用的底物范围,以及真菌如何与细菌合作降解复杂的有机物,包括碳氢化合物。已知真菌参与难降解有机物的降解和金属的循环,并产生具有有趣特性的新型代谢物。这个项目告诉我们地球上微生物生命的不同谱系的起源,海洋地下碳循环的程度,生命的极限,生命如何适应环境变化,以及真菌加速复杂碳氢化合物生物降解的潜力。考虑到潜在的地下生物圈的范围,真菌可能在全球营养循环中发挥重要作用。由该项目创建的真菌分离物的培养物收集将可用于其他感兴趣的研究人员探索其生态学和新特性,并且还可能产生对基础真菌谱系的见解。这些地球化学和潜在的进化结果对其他研究学科,教育工作者和学生都非常感兴趣。该项目的K-16教育计划利用旨在增加代表性不足的本科生参与研究的计划。高中生、本科生、研究生和博士后都参与了这项研究。 一个与当地一所高中合作的科学艺术项目正在社区图书馆沿着,同时展出的还有关于海洋真菌及其生态作用的教育材料。该项目研究了地下沉积物样品中真菌和共同居住的细菌和古菌的丰度,多样性和分布如何变化,表现出广泛的原位温度和压力,细胞的活性部分是沿着这些梯度,以及真菌是否/如何通过与细菌代谢相互作用来影响生物圈中的碳循环,以分解碳氢化合物底物。该项目正在评估这一活跃的地下热液生物圈中原地微生物的活动,采用先进的分子方法和基于培养的富集和微生物分离研究相结合的方法,对从瓜伊马斯盆地8个地点广泛收集的样品进行研究,这些地点的温度分布、是否存在古老的埋藏岩浆岩床以及地球化学条件各不相同。研究人员正在检查1)使用新的生物正交非规范氨基酸标记(BONCAT)方法分选的活性细胞的标记基因和宏基因组,2)使用显微镜,“元组学”和脂质生物标志物分析,细菌,古细菌和真菌细胞及其标记基因沿着深度和地球化学梯度的分布,3)真菌分离物的底物使用,4)代谢物池,营养物,碳氢化合物的深度真菌对复杂有机物的代谢(以及真菌和细菌之间的同养型)使用培养物中RNA的时程稳定同位素探测-该奖项反映了NSF的法定使命,并被认为值得通过利用基金会的智力价值和更广泛的影响进行评估来支持审查标准。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Microbial gene expression in Guaymas Basin subsurface sediments responds to hydrothermal stress and energy limitation
- DOI:10.1038/s41396-023-01492-z
- 发表时间:2023-09-01
- 期刊:
- 影响因子:11
- 作者:Mara, Paraskevi;Zhou, Ying-Li;Edgcomb, Virginia
- 通讯作者:Edgcomb, Virginia
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Andreas Teske其他文献
Nitrogen isotope homogenization of dissolved ammonium with depth and <sup>15</sup>N enrichment of ammonium during incorporation into expandable layer silicates in organic-rich marine sediment from Guaymas Basin, Gulf of California
- DOI:
10.1016/j.chemgeo.2024.122203 - 发表时间:
2024-10-20 - 期刊:
- 影响因子:
- 作者:
Toshiro Yamanaka;Arisa Sakamoto;Kanon Kiyokawa;Jaeguk Jo;Yuji Onishi;Yoshihiro Kuwahara;Ji-Hoon Kim;Lucie C. Pastor;Andreas Teske;Daniel Lizarralde;Tobias W. Höfig;Ivano W. Aiello;Jeanine L. Ash;Diana P. Bojanova;Martine D. Buatier;Virginia P. Edgcomb;Christophe Y. Galerne;Swanne Gontharet;Verena B. Heuer;Shijun Jiang - 通讯作者:
Shijun Jiang
Uncultured archaea in deep marine subsurface sediments: have we caught them all?
深海海洋地下沉积物中的未培养古细菌:我们已经把它们全部捕获了吗?
- DOI:
10.1038/ismej.2007.90 - 发表时间:
2007-11-08 - 期刊:
- 影响因子:10.000
- 作者:
Andreas Teske;Ketil B Sørensen - 通讯作者:
Ketil B Sørensen
Nitrogen isotope homogenization of dissolved ammonium with depth and sup15/supN enrichment of ammonium during incorporation into expandable layer silicates in organic-rich marine sediment from Guaymas Basin, Gulf of California
加利福尼亚湾瓜伊马斯盆地富含有机质的海洋沉积物中,溶解态铵随深度的氮同位素均一化以及铵在纳入膨胀层硅酸盐过程中的δ¹⁵N富集
- DOI:
10.1016/j.chemgeo.2024.122203 - 发表时间:
2024-10-20 - 期刊:
- 影响因子:3.600
- 作者:
Toshiro Yamanaka;Arisa Sakamoto;Kanon Kiyokawa;Jaeguk Jo;Yuji Onishi;Yoshihiro Kuwahara;Ji-Hoon Kim;Lucie C. Pastor;Andreas Teske;Daniel Lizarralde;Tobias W. Höfig;Ivano W. Aiello;Jeanine L. Ash;Diana P. Bojanova;Martine D. Buatier;Virginia P. Edgcomb;Christophe Y. Galerne;Swanne Gontharet;Verena B. Heuer;Shijun Jiang;Guangchao Zhuang - 通讯作者:
Guangchao Zhuang
Characteristics and Evolution of sill-driven off-axis hydrothermalism in Guaymas Basin – the Ringvent site
瓜伊马斯盆地-环喷口地点的岩席驱动的离轴热液活动的特征和演化
- DOI:
10.1038/s41598-019-50200-5 - 发表时间:
2019-09-25 - 期刊:
- 影响因子:3.900
- 作者:
Andreas Teske;Luke J. McKay;Ana Christina Ravelo;Ivano Aiello;Carlos Mortera;Fernando Núñez-Useche;Carles Canet;Jeffrey P. Chanton;Benjamin Brunner;Christian Hensen;Gustavo A. Ramírez;Ryan J. Sibert;Tiffany Turner;Dylan White;Christopher R. Chambers;Andrew Buckley;Samantha B. Joye;S. Adam Soule;Daniel Lizarralde - 通讯作者:
Daniel Lizarralde
IODP Expedition 385: Guaymas Basin Tectonics and Biosphere
IODP 385 号探险队:瓜伊马斯盆地构造和生物圈
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Yuki Morono;Andreas Teske;Daniel Lizarralde;Tobias W Hofig;IODP EXPEDITION 385 SCIENTISTS - 通讯作者:
IODP EXPEDITION 385 SCIENTISTS
Andreas Teske的其他文献
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{{ truncateString('Andreas Teske', 18)}}的其他基金
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
合作研究:瓜伊马斯盆地碳氢化合物生态系统中的热液真菌
- 批准号:
1829680 - 财政年份:2018
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: Next generation physiology: a systems-level understanding of microbes driving carbon cycling in marine sediments
合作研究:下一代生理学:对驱动海洋沉积物碳循环的微生物的系统级理解
- 批准号:
1817381 - 财政年份:2018
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
合作研究:瓜伊马斯盆地热液沉积物中微生物碳循环及其与硫和氮转化的相互作用
- 批准号:
1357238 - 财政年份:2014
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
RAPID proposal: Site characterization cruise to document the active and extensive subsurface biosphere in the Guaymas Basin
RAPID 提案:现场表征巡航,记录瓜伊马斯盆地活跃且广泛的地下生物圈
- 批准号:
1449604 - 财政年份:2014
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
RAPID: The Microbial Response to the Deepwater Horizon Oil Spill
RAPID:微生物对深水地平线漏油事件的反应
- 批准号:
1045115 - 财政年份:2010
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
MO: COLLABORATIVE RESEARCH: A MICROBIAL OBSERVATORY EXAMINING MICROBIAL ABUNDANCE, DIVERSITY, ASSOCIATIONS AND ACTIVITY AT SEAFLOOR BRINE SEEPS
MO:合作研究:微生物观测站检查海底盐水渗漏处的微生物丰度、多样性、关联性和活动
- 批准号:
0801742 - 财政年份:2008
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Microbial carbon and sulfur cycling in the hydrothermally altered sediments of Guaymas Basin
瓜伊马斯盆地热液蚀变沉积物中的微生物碳和硫循环
- 批准号:
0647633 - 财政年份:2007
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Proposal: Site Survey and Initial Characterization of North Pond: Microbiology, Biogeochemistry, Seismic Profiles
合作提案:北池塘的现场调查和初步表征:微生物学、生物地球化学、地震剖面
- 批准号:
0727175 - 财政年份:2007
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: Drilling Site Survey-Life in Subseafloor Sediments of the South Pacific Gyre
合作研究:钻探现场调查——南太平洋环流海底沉积物中的生命
- 批准号:
0527039 - 财政年份:2005
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
LExEn: Hyperthermophiles of the Hydrothermal Vent Subsurface and Their Environmental Tolerance
LExEn:热液喷口地下的超嗜热生物及其环境耐受性
- 批准号:
0085534 - 财政年份:2000
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
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相似海外基金
Collaborative Research: IODP-enabled Insights into Fungi and Their Metabolic Interactions with Other Microorganisms in Deep Subsurface Hydrothermal Sediments
合作研究:借助 IODP 深入了解深层地下热液沉积物中的真菌及其与其他微生物的代谢相互作用
- 批准号:
2046056 - 财政年份:2021
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: IODP-enabled Insights into Fungi and Their Metabolic Interactions with Other Microorganisms in Deep Subsurface Hydrothermal Sediments
合作研究:借助 IODP 深入了解深层地下热液沉积物中的真菌及其与其他微生物的代谢相互作用
- 批准号:
2046799 - 财政年份:2021
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Standard Grant
Collaborative Research: On how the Bengal-Nicobar fan deposition influenced carbonate cementation in the incoming sediment to the Sumatra subduction zone (IODP Exp 362)
合作研究:孟加拉-尼科巴扇沉积如何影响苏门答腊俯冲带传入沉积物中的碳酸盐胶结作用(IODP Exp 362)
- 批准号:
1833269 - 财政年份:2018
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: On how the Bengal-Nicobar fan deposition influenced carbonate cementation in the incoming sediment to the Sumatra subduction zone (IODP Exp 362)
合作研究:孟加拉-尼科巴扇沉积如何影响苏门答腊俯冲带传入沉积物中的碳酸盐胶结作用(IODP Exp 362)
- 批准号:
1833296 - 财政年份:2018
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: The four-dimensional distribution of magmatism during the growth of lower oceanic crust: High precision U-Pb dating of IODP Hole U1473A, Atlantis Bank, SWIR
合作研究:下洋地壳生长过程中岩浆活动的四维分布:IODP孔U1473A的高精度U-Pb测年,亚特兰蒂斯浅滩,SWIR
- 批准号:
1636678 - 财政年份:2016
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: The four-dimensional distribution of magmatism during the growth of lower oceanic crust: High precision U-Pb dating of IODP Hole U1473A, Atlantis Bank, SWIR
合作研究:下洋地壳生长过程中岩浆活动的四维分布:IODP孔U1473A的高精度U-Pb测年,亚特兰蒂斯浅滩,SWIR
- 批准号:
1636703 - 财政年份:2016
- 资助金额:
$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: Geochemistry of IODP Site 1438 and West Philippine Basin Volcanic Rocks: Constraints on Subduction Initiation and the Early Development of the Izu-Bonin-Mar
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- 批准号:
1537861 - 财政年份:2015
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$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: Geochemistry of IODP Site 1438 and West Philippine Basin Volcanic Rocks: Constraints on Subduction Initiation and the Early Development of the Izu-Bonin-Mar
合作研究:IODP 1438号站点和西菲律宾盆地火山岩的地球化学:俯冲起始和伊豆博宁马早期发育的制约因素
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1537135 - 财政年份:2015
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$ 3.86万 - 项目类别:
Standard Grant
Collaborative Research: Resolving Centennial-Scale Environmental Change and Geomagnetic Field Variability From Iberian Margin Sediment Drifts Cored on IODP Expedition 339
合作研究:解决以 IODP 339 号探险为核心的伊比利亚边缘沉积物漂移带来的百年规模的环境变化和地磁场变化
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1334163 - 财政年份:2013
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Standard Grant
Collaborative Research: Resolving Centennial-Scale Environmental Change and Geomagnetic Field Variability From Iberian Margin Sediment Drifts Cored on IODP Expedition 339
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1335233 - 财政年份:2013
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Standard Grant