MRI: Acquisition of a High Resolution Mass Spectrometer, Liquid Chromatography System to Support Research and Teaching within the Sciences

MRI：购置高分辨率质谱仪、液相色谱系统以支持科学研究和教学

• 批准号: 2116635
• 负责人: Patrick Hare
• 金额: $ 33.51万
• 依托单位: Northern Kentucky University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2116635&HistoricalAwards=false
• 关键词: MRI; Acquisition; Resolution; Mass; Spectrometer

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Northern Kentucky University is acquiring a benchtop high performance quadrupole tandem mass spectrometer equipped with a high pressure liquid chromatograph and a nitrogen generator to support Professor Molly Hopper and colleagues Patrick Hare and Christine Curran. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used by collaborators from nearby institutions.The award of the quadrupole tandem mass spectrometer is aimed at enhancing research and education at all levels. It is especially useful for identification of metabolites after crop exposure to environmental stressors and for monitoring the metabolomic impact of gut microbiome changes following activation of the aryl hydrocarbon receptor (AHR) following benzo[a]pyrene exposure from coal tar, tobacco smoke and many foods, especially grilled meats. The instrumentation is also used for understanding the photophysics and photochemistry of estrogens and their photodegradation products and the type of compounds formed during cyclization prior to the preparation of analytically pure compounds used for characterization in phenylethynylene- and annulene-oxacalixarene. The instrument also serves researchers carrying out hybrids synthesis and those synthesizing diarylheptanoid analogues to obtain a selective microsomal prostaglanding E synthase-1 (mPGES-1) enzyme inhibitor with low nanomolar potency in vitro. The instrumentation is also used for studying palladium-catalyzed direct alpha-C(sp3-hybridized)-heteroarylation of carbonyl compounds for use in organic chemistry, medicinal chemistry and chemical industries.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器计划支持。北方肯塔基州大学正在购买一台台式高性能四极杆串联质谱仪，配备有高压液相色谱仪和氮气发生器，以支持莫莉·霍珀教授和同事帕特里克黑尔和克莉丝汀柯伦。一般来说，质谱（MS）是用于识别和表征复杂样品中少量化学物质的关键分析方法之一。带有液相色谱仪的仪器通过在化合物到达质谱仪之前分离化合物的混合物来提供额外的结构鉴定能力。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与这种现代分析技术来扩大参与。授予四极杆串联质谱仪的目的是加强各级的研究和教育。它特别适用于鉴定作物暴露于环境压力后的代谢物，以及监测煤焦油、烟草烟雾和许多食物（特别是烤肉）中苯并[a]芘暴露后芳香烃受体（AHR）激活后肠道微生物组变化的代谢组学影响。该仪器还用于了解雌激素及其光降解产物的光化学和光化学，以及在制备用于表征苯乙炔基和轮烯氧杂杯芳烃的分析纯化合物之前环化过程中形成的化合物类型。该仪器还为研究人员进行杂交合成和那些合成二芳基庚烷类似物，以获得选择性微粒体结合E合酶-1（mPGES-1）酶抑制剂，在体外具有低纳摩尔效力。该仪器还用于研究钯催化的直接α-C（sp3-杂化）-羰基化合物的杂芳基化反应，用于有机化学、药物化学和化学工业。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。