Collaborative Research: EDGE FGT: Genome-wide knock-out mutant libraries for the microcrustacean Daphnia
合作研究:EDGE FGT:微甲壳动物水蚤的全基因组敲除突变体文库
基本信息
- 批准号:2220695
- 负责人:
- 金额:$ 110万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-08-15 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Mutating the DNA sequences of genes of interest is a valuable approach for understanding their biological functions in diverse organisms. In contrast to established model systems, large collections of gene knock-out mutants are not widely available in emerging model organisms. This limitation greatly diminishes the power of emerging model systems for making novel scientific discoveries. To address the issue, this project will develop a large collection of gene knock-out mutants using CRISPR gene editing in three species of the freshwater microcrustacean Daphnia (D. magna, D. pulex, and D. pulicaria), which is an emerging model system in ecological genomics, evolutionary genomics, and environmental genomics. The Daphnia knock-out mutants will provide unprecedented opportunities for the scientific community to characterize the biological functions of the genes. The project will also disseminate technical expertise for CRISPR gene editing in Daphnia through free workshops, targeting biologists at different career stages including undergraduates, graduates, postdoctoral researchers, and faculty. Furthermore, the project will develop an innovative genomics curriculum to enable hands-on research in the classroom and enhance the education experience of diverse undergraduate students. The plan is to knock out genes in Daphnia, including many candidate genes that may play critical roles in acclimation, adaptation, and evolution of these species. Individual knock-out mutant lines will be generated, maintained in the laboratory, and freely shared with the research community. Each mutant line will be whole-genome sequenced to characterize its genomic background. In collaboration with the global Daphnia research community, this project will evaluate the functional changes in knock-out mutants at both genomic and organism levels to validate the role of the genes in various environmental settings and to infer gene interaction and gene regulation networks. Lastly, a free, public-facing database will be developed to host the genomic and phenotypic data of the mutant lines, to promote and manage the distribution of Daphnia mutants, and to distribute experimental protocols and reagents.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
突变感兴趣的基因的DNA序列是了解它们在不同生物体中的生物学功能的有价值的方法。与已建立的模型系统相反,大量的基因敲除突变体在新兴的模式生物中并不广泛存在。这种局限性大大削弱了新兴模型系统的力量,使新的科学发现。为了解决这个问题,该项目将使用CRISPR基因编辑在三种淡水微甲壳动物水蚤(D. magna,D. pulex和D. Pulicaria),是生态基因组学、进化基因组学和环境基因组学中的新兴模式系统。水蚤敲除突变体将为科学界提供前所未有的机会来表征基因的生物学功能。该项目还将通过免费研讨会传播水蚤CRISPR基因编辑的技术专业知识,针对不同职业阶段的生物学家,包括本科生,研究生,博士后研究人员和教师。此外,该项目将开发一个创新的基因组学课程,使动手研究在课堂上,并提高不同的本科生的教育经验。 该计划是敲除水蚤中的基因,包括许多可能在这些物种的适应,适应和进化中发挥关键作用的候选基因。将在实验室中产生、维持单个敲除突变株系,并与研究界自由分享。将对每个突变系进行全基因组测序,以表征其基因组背景。该项目与全球水蚤研究界合作,将在基因组和生物体水平评估敲除突变体的功能变化,以验证基因在各种环境环境中的作用,并推断基因相互作用和基因调控网络。最后,将建立一个免费的、面向公众的数据库,用于存储突变品系的基因组和表型数据,促进和管理水蚤突变体的分布,并分发实验方案和试剂。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Sen Xu其他文献
Negative thermal expansion of Ca2RuO4 with oxygen vacancies
氧空位 Ca2RuO4 的负热膨胀
- DOI:
10.1088/1674-1056/ab8a36 - 发表时间:
2020-07 - 期刊:
- 影响因子:1.7
- 作者:
Sen Xu;Yangming Hu;Yuan Liang;Chenfei Shi;Yuling Su;Juan Guo;Qilong Gao;Mingju Chao;Erjun Liang - 通讯作者:
Erjun Liang
Non-iterative constrained compressive beamforming
- DOI:
10.1016/j.measurement.2023.112730 - 发表时间:
2023 - 期刊:
- 影响因子:
- 作者:
Qijia Guo;Haifeng Zhao;Tian Zhou;Zhongmin Wang;Sen Xu;Hong-Liang Cui - 通讯作者:
Hong-Liang Cui
Cause analysis of spontaneous combustion in an ammonium nitrate emulsion explosive
- DOI:
10.1016/j.jlp.2016.05.010 - 发表时间:
2016-09-01 - 期刊:
- 影响因子:
- 作者:
Sen Xu;Liu Tan;Jing-ping Liu;Xiang Chen;Wei Jiang;Yuan Chen;Da-bin Liu - 通讯作者:
Da-bin Liu
Flow paths control on spatiotemporal weathering dynamics in high-relief regions, eastern Qinghai-Tibet Plateau: Insight from dissolved Li-Sr-O isotopes
青藏高原东部高地势地区水流路径对时空风化动力学的控制:来自溶解态锂 - 锶 - 氧同位素的启示
- DOI:
10.1016/j.chemgeo.2025.122854 - 发表时间:
2025-09-05 - 期刊:
- 影响因子:3.600
- 作者:
Tingting Ma;Jun Zhong;Si-Liang Li;Sen Xu;Han Luo;Cong-Qiang Liu - 通讯作者:
Cong-Qiang Liu
Seasonal variations and hydrological management regulate nutrient transport in cascade damming: Insights from carbon and nitrogen isotopes
- DOI:
10.1016/j.watres.2024.122894 - 发表时间:
2025-03-01 - 期刊:
- 影响因子:
- 作者:
Yujing Yang;Wanfa Wang;Shengde Yu;Yuanbi Yi;Sen Xu;Yuanzhi Yao;Jun Zhong;Wenhong Shi;Sainan Chen;Qixin Wu;Zuhong Ou;Hu Ding;Si-Liang Li - 通讯作者:
Si-Liang Li
Sen Xu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Sen Xu', 18)}}的其他基金
Collaborative Research: EDGE FGT: Genome-wide knock-out mutant libraries for the microcrustacean Daphnia
合作研究:EDGE FGT:微甲壳动物水蚤的全基因组敲除突变体文库
- 批准号:
2324639 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Continuing Grant
CAREER: Understanding the evolution and genetic basis of meiotic recombination rate
职业:了解减数分裂重组率的进化和遗传基础
- 批准号:
2348390 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Continuing Grant
CAREER: Understanding the evolution and genetic basis of meiotic recombination rate
职业:了解减数分裂重组率的进化和遗传基础
- 批准号:
2042490 - 财政年份:2021
- 资助金额:
$ 110万 - 项目类别:
Continuing Grant
相似国自然基金
Research on Quantum Field Theory without a Lagrangian Description
- 批准号:24ZR1403900
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
Cell Research
- 批准号:31224802
- 批准年份:2012
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research
- 批准号:31024804
- 批准年份:2010
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research (细胞研究)
- 批准号:30824808
- 批准年份:2008
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
- 批准号:10774081
- 批准年份:2007
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
Collaborative Research: Conference: DESC: Type III: Eco Edge - Advancing Sustainable Machine Learning at the Edge
协作研究:会议:DESC:类型 III:生态边缘 - 推进边缘的可持续机器学习
- 批准号:
2342498 - 财政年份:2024
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
Collaborative Research: Learning for Safe and Secure Operation of Grid-Edge Resources
协作研究:学习电网边缘资源的安全可靠运行
- 批准号:
2330154 - 财政年份:2024
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
Collaborative Research: Conference: DESC: Type III: Eco Edge - Advancing Sustainable Machine Learning at the Edge
协作研究:会议:DESC:类型 III:生态边缘 - 推进边缘的可持续机器学习
- 批准号:
2342497 - 财政年份:2024
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
Collaborative Research: Design Decisions under Competition at the Edge of Bounded Rationality: Quantification, Models, and Experiments
协作研究:有限理性边缘竞争下的设计决策:量化、模型和实验
- 批准号:
2419423 - 财政年份:2024
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
Collaborative Research: Learning for Safe and Secure Operation of Grid-Edge Resources
协作研究:学习电网边缘资源的安全可靠运行
- 批准号:
2330155 - 财政年份:2024
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
Collaborative Research: SHF: Small: Quasi Weightless Neural Networks for Energy-Efficient Machine Learning on the Edge
合作研究:SHF:小型:用于边缘节能机器学习的准失重神经网络
- 批准号:
2326895 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
Collaborative Research: SHF: Medium: Towards Harmonious Federated Intelligence in Heterogeneous Edge Computing via Data Migration
协作研究:SHF:中:通过数据迁移实现异构边缘计算中的和谐联邦智能
- 批准号:
2312617 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Continuing Grant
Collaborative Research: NeTS: Medium: Towards High-Performing LoRa with Embedded Intelligence on the Edge
协作研究:NeTS:中:利用边缘嵌入式智能实现高性能 LoRa
- 批准号:
2312676 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Standard Grant
III: Medium: Collaborative Research: Integrating Large-Scale Machine Learning and Edge Computing for Collaborative Autonomous Vehicles
III:媒介:协作研究:集成大规模机器学习和边缘计算以实现协作自动驾驶汽车
- 批准号:
2348169 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Continuing Grant
Collaborative Research: EDGE CMT: Genomic and molecular bases of pollination syndrome evolution in monkeyflowers
合作研究:EDGE CMT:猴花授粉综合征进化的基因组和分子基础
- 批准号:
2319721 - 财政年份:2023
- 资助金额:
$ 110万 - 项目类别:
Continuing Grant