CAREER: 3D Printing High Lipid Content Cultivated Meat to Minimize Livestock Environmental Impacts

事业：3D 打印高脂质含量的栽培肉以最大限度地减少牲畜环境影响

• 批准号: 2236998
• 负责人: Rosalyn Abbott-Beauregard
• 金额: $ 60万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-15 至 2028-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2236998&HistoricalAwards=false
• 关键词: CAREER; 3D; Printing; Lipid; Content

The current agricultural supply chain will be unable to sustainably meet the world’s increasing demand for animal proteins. Currently, the majority of livestock are reared in concentrated animal feeding operations causing environmental, public health, and food security concerns. An emerging option is to replace conventionally produced meat with meat-like tissues grown in the lab (i.e., cultured meat). Cultured meat can help to reduce the environmental, public health, and food security issues posed by commercial farming. However, major scientific, technological, and educational barriers related to consumer acceptance must be overcome. Developing cultured meat products (i.e., ground meat and/or steaks) that have the texture, flavor, and familiarity of conventional meat products will be critical to consumer acceptance. Current cultured meat technologies are expensive and have focused on ground meat products that do not re-create the three-dimensional (3D) tissue structures of animal steaks, especially with regards to the distribution of fat in these tissues. Therefore, the goal of this CAREER project is to establish a fundamental understanding of the biological dynamics of fat accumulation in muscle and develop 3D bioprinting techniques and parameters to create cultured meat products that match the properties and structures of conventional meats. 3D bioprinting would add minimal production costs while significantly increasing the price point of the final cultured product, improving the financial viability of the industry. In parallel, this proposal seeks to engage in educational and public outreach activities to increase consumer awareness of the current animal protein supply chain’s critical weaknesses and the biomedical engineering solutions to relieve these, drive consumer acceptance, and grow the cellular agriculture research community. The investigator’s long-term research goals involve 3D printing of biological tissues, mechanistic studies of disease processes, and regenerative medicine. Towards this goal, this CAREER project seeks to enhance consumer acceptance and palatability of cultured meats by accurately reproducing the texture, properties, and costs associated with conventional meat products. The Research Plan is organized under two objectives: (1) Obtain a fundamental understanding of intramuscular fat accumulation and (2) Print viable, lipid-laden adipocytes alone and into the intramuscular space. These objectives will provide an open-source foundation for the cultured meat industry and future work in human disease modelling, regenerative medicine, and reconstructive medicine. The proposed studies will (1) develop conditions for co-culturing mature adipocytes and skeletal muscle cells that have not been achieved or reported, (2) make significant contributions to creating new models of biological mechanisms for intramuscular fat accumulation, and (3) establish parameters for the 3D bioprinting of tissues that contain both lipid-laden adipocytes and skeletal muscle cells. These 3D printed tissues should be mechanically and compositionally similar to their biological counterparts and will significantly improve the fundamental research and application-driven capabilities of technical personnel in cellular agriculture and biomedical engineering.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

当前的农业供应链将无法持续满足世界对动物蛋白日益增长的需求。目前，大多数牲畜都是在集中饲养场饲养，引起环境、公共卫生和粮食安全问题。一种新兴的选择是用实验室培养的类肉组织（即培养肉）替代传统生产的肉类。人造肉有助于减少商业化养殖带来的环境、公共卫生和粮食安全问题。然而，必须克服与消费者接受度相关的主要科学、技术和教育障碍。开发具有传统肉制品质地、风味和熟悉度的培养肉制品（即碎肉和/或牛排）对于消费者接受度至关重要。目前的培养肉技术价格昂贵，并且主要集中在碎肉产品上，这些产品无法重现动物牛排的三维 (3D) 组织结构，尤其是这些组织中的脂肪分布。因此，该 CAREER 项目的目标是建立对肌肉中脂肪积累的生物动力学的基本了解，并开发 3D 生物打印技术和参数，以创建与传统​​肉类的特性和结构相匹配的培养肉产品。 3D生物打印将增加最小的生产成本，同时显着提高最终培养产品的价格，从而提高该行业的财务生存能力。与此同时，该提案旨在开展教育和公共宣传活动，以提高消费者对当前动物蛋白供应链的关键弱点以及缓解这些弱点的生物医学工程解决方案的认识，提高消费者的接受度，并发展细胞农业研究社区。 研究人员的长期研究目标涉及生物组织的 3D 打印、疾病过程的机制研究和再生医学。 为了实现这一目标，该 CAREER 项目旨在通过准确再现与传统肉制品相关的质地、特性和成本来提高消费者对培养肉的接受度和适口性。 该研究计划的组织目标有两个：(1) 获得对肌内脂肪积累的基本了解；(2) 单独打印活的、充满脂质的脂肪细胞并将其放入肌内空间。 这些目标将为培养肉行业以及人类疾病建模、再生医学和重建医学的未来工作提供开源基础。拟议的研究将(1)开发尚未实现或报道的共培养成熟脂肪细胞和骨骼肌细胞的条件，(2)为创建肌内脂肪积累的生物机制的新模型做出重大贡献，(3)建立含有脂质脂肪细胞和骨骼肌细胞的组织的3D生物打印参数。这些3D打印组织在机械和成分上应与生物组织相似，并将显着提高细胞农业和生物医学工程技术人员的基础研究和应用驱动能力。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。