CAREER:Contaminants of Emerging Concern in Treated Wastewater Impact Plant-Associated Microbes and Plant Stress Responses

这项研究将探讨新出现的关注化学品（CEC）如何影响大豆与土壤细菌的关系，从而影响大豆产量。蚕豆是美国的主要作物，每年产生超过460亿美元的农业收入。菜豆是一种受欢迎的轮作作物，因为它们通过与土壤细菌属慢生根瘤菌的互利关系增加土壤氮。种植大豆减少了化肥的使用，而不影响与大豆轮作的粮食作物的产量。了解CEC对直接影响产量的因素的影响，例如大豆-慢生根瘤菌相互作用的建立，接种和植物生长，将为水区和种植者的回收废水质量决策提供信息，以确保可持续的农业生产力。拟议的研究还将提供指导的研究经验和培训，通过加州大学-滨江科学与工程研究[上升]计划招募的代表性不足的少数民族（URM）的学生。为期10周的夏季研究模块旨在向学生传授植物-微生物相互作用，并为他们提供劳动力所需的实验室经验。一个本科URM学生将从这个组中招募加入PI的实验室作为付费学生助理，并帮助培训下一年的夏季研究培训学生。在与科学和技术的成功合作：与角色模型（姐妹篇）计划的参与，URM女孩和教师从大学高地中学，在滨江CA，将获得动手培训植物微生物相互作用的好处，并就如何CEC可以破坏它们。尽管有压倒性的证据表明，新出现的关注（CEC）的化学品改变微生物多样性，研究CEC对植物微生物相互作用的影响是至关重要的植物健康和生产力是缺乏的。这项研究将首次利用大豆-慢生根瘤菌的相互作用来展示CEC对具有巨大经济价值的共生相互作用的影响。PI将使用加利福尼亚州南部常见的四种CEC来研究CEC如何影响大豆根瘤、根和根际微生物组。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The proposed study will investigate how chemicals of emerging concern (CECs) affect soybean associations with soil bacteria and consequently soybean yield. Soybeans are a major crop in the USA, generating more than $46 billion annual farm revenue. Soybeans are a popular rotation crop because they increase soil nitrogen through a mutualistic relationship with Bradyrhizobium, a genus of soil bacteria. Planting soybeans reduces chemical fertilizers application without compromising the yield of grain crops planted in rotation with soybeans. Understanding the effects of CECs on factors that directly impact yield, such as establishment of the soybean-Bradyrhizobium interaction, nodulation, and plant growth, will inform recycled wastewater quality decisions by water districts and growers in efforts to ensure sustainable agricultural productivity. The proposed research will also provide mentored research experience and training for underrepresented minority (URM) students recruited through the University of California - Riverside Research In Science and Engineering [RISE] Program. The 10-week summer research module is designed to teach the students about plant-microbe interactions and provide them with lab experience needed in the workforce. One undergraduate URM students will be recruited from this group to join the PI’s lab as paid student assistants and to help in training the next year's Summer Research Training students. In collaboration with the Success in Science and Technology: Engagement with Role-Models (SISTERS) program, URM girls and teachers from University Heights Middle School, in Riverside CA, will get hands-on training on the benefits of plant-microbe interactions and on how CECs can disrupt them. Despite overwhelming evidence that chemicals of emerging concern (CECs) alter microbial diversity, research regarding the impacts of CECs on plant-microbe interactions that are critical to plant health and productivity is lacking. For the first time, the proposed study will show the impacts of CECs on symbiotic interactions of great economic value using the soybean-Bradyrhizobium interaction. Using four CECs that are frequently found in southern Califormia the PI will investigate how CECs affect the soybean nodule, root and rhizosphere microbiome.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.