2D polarization resolved optical spectroscopy for exploration of conformation, energy transfer and aggregation of macromolecules (conjugated polymers and proteins) within different environments under the goal to contribute to the improvement of organic bu

本计画将利用最新发展的二维偏光影像技术（2D POLIM）来探讨大分子的构象与聚集。研究的目的是建立新的见解与前景的有机电子学中的应用改进所研究的大分子的组织。研究对象，一方面，将共轭聚合物，这是由于其电子和光学性质，适合于有机电子学。应用二维POLIM的液晶（LC）矩阵共轭聚合物的稀释溶液将使我们能够评估的拓扑结构的聚合物链/颗粒和测量这些对象内的能量传递的程度。与无定形聚合物基质相比，LC提供了已知的局部结构，其可以通过温度和电场来控制。链的大小，链构象，聚集度和能量转移效率之间的明确的相关性的建立仍然缺乏。这些知识对于有机电子尤其重要，其中聚合物链形态似乎是器件性能的关键。另一方面，在活细胞和体外的生物聚合物的调查，预计将提供新的见解，其功能和组织的复杂途径。功能结构在细胞生物学中起着重要作用。通过将我们的研究活动扩展到生物材料的研究，我们期望为有机太阳能电池的改进找到可行的线索。初步研究证明，2D POLIM的力量，以解决生物医学样品中的结构是上级传统的荧光光谱技术。在与瓦伦堡神经科学中心，生物医学中心，隆德大学的合作将探讨蛋白质的聚集状态。2DPOLIM能够分辨能量传递和粒子取向，为研究蛋白质相互作用提供了一种比其他荧光显微技术更直接的方法，从这两种材料的研究中，我们期望为有机体异质结太阳能电池的改进提供新的见解。与瑞典有机电子中心（COE）的现有合作将为我们提供材料，以直接测试我们研究结果所激发的结构和材料成分。

This project will explore conformation and aggregation of macromolecules using recently developed 2D polarization imaging techniques (2D POLIM). The aim of the investigation is to establish new insight into the organization of the studied macromolecules with the prospect for improved applications in organic electronics. The studied objects, one the one hand, will be conjugated polymers, which due to their electronic and optical properties are suitable for organic electronics. Application of 2D POLIM to diluted solutions of conjugated polymers in liquid crystal (LC) matrices will enable us to evaluate the topology of the polymer chains/particles and measure the extend of energy transfer within these objects. In contrast to amorphous polymer matrices, LCs provide a known local structure, which can be controlled by temperature and electric field. Establishing of unambiguous correlations between the chain size, chain conformation, degree of aggregation and energy transfer efficiency is still lacking. Such knowledge is especially important for organic electronics where the polymer chain morphology seems to be the key for the device performance. On the other hand, investigation of biopolymers in living cells and in vitro is expected to provide new insight in the complex pathways of their functionality and organization. Functional structures play a prominent role in cell biology. By extending our research activities to the investigation of biological materials, we expect to find viable clues for the improvement of organic solar cells. Preliminary investigation proved the power of 2D POLIM to resolve structures in biomedical samples to be superior to conventional fluorescence spectroscopy techniques. In cooperation with the Wallenberg Neuroscience Center, Bio-Medical Center, Lund University aggregation states in proteins will be explored. The ability of 2D POLIM to resolve energy transfer together with particle orientations, provides a more direct approach on interaction of proteins than other fluorescence microscopy techniques.From the investigations of both materials, we expect new insight for the improvement of organic bulk heterojunction solar cells. The already existing collaboration with the Swedish Center for Organic Electronics (COE) will provide us with materials to directly test structures and material compositions motivated by the results from our studies.