The Role of Meiotic-Stage Non-Coding RNA in the Modulation of Anther & Pollen Development in Grasses

减数分裂阶段非编码 RNA 在花药调节中的作用

基本信息

批准号：
1754097
负责人：
Blake Meyers
金额：
$ 352.1万
依托单位：
Donald Danforth Plant Science Center
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-15 至 2024-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754097&HistoricalAwards=false
关键词：
Role Meiotic Stage Non Coding

项目摘要

This project will investigate the lifecycle and functions of a class of small RNAs that support pollen development in grass flowers, specifically in anthers. The experiments focus on the biogenesis, precise localization, and quantification of these 24-nucleotide RNAs, the proteins that utilize them for anther functions, and their role in plant fertility. Prior work demonstrated that these small RNAs are required for robust male fertility under even slight temperature stress: without them anther development fails. The project will focus on maize anthers because it is easy to determine accurately what developmental stage they are in, they are easy to dissect, there are numerous developmental mutants that affect anthers for which the gene has been isolated, and because understanding male fertility is important to the production of hybrid corn seed. A more detailed understanding of the molecular basis of pollen development enables improvements in seed production, including hybrid seeds; in the grasses, hybrid corn and rice have significantly boosted world food production. Outcomes of this project could include discovering new genetic pathways for more precise control of male fertility in plants, and plants with fertility that is more resilient to environmental change. The project will utilize plant genomics and targeted mutagenesis, cutting-edge imaging techniques, bioinformatics, with a focus on developmental biology. Broader impacts of the project include training of students in plant and computational biology via continued integration with long-running and successful undergraduate and high school internship programs. The project will focus specifically on the class of 24-nt phased, secondary siRNAs, known as "24-nt phasiRNAs" that are highly enriched in meiotic-stage anthers. The work will be done in maize as the primary species for analysis because there are significant, existing genetic and molecular resources. The project will address several important questions. Do meiotic cells differ from somatic cells in the accumulation of small RNAs and their functions? Which transcription factors guide the transitions required for setting cell fate and differentiation of cell type-specific properties that include phasiRNAs? How are meiotic-stage small RNAs deployed to guide anther cell fate and differentiation? Where and when do the microRNA triggers, precursor mRNAs, and biogenesis or effector proteins accumulate? The project will characterize phenotypes and molecular genetic analysis of loss-of-function knock-out mutations that impact phasiRNAs, including mutations of the Dicer-like5 (Dcl5) gene, for which preliminary data demonstrate male sterility. Project members will be trained broadly in plant small RNA biology and genomics, cell biology and microscopy, and computational methods through their individual and group projects in the analysis of the localization, biogenesis, and biochemistry of small RNAs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将研究一类小型RNA的生命周期和功能，这些RNA支持草花，特别是花药中的花粉发育。该实验的重点是对这些24-核苷酸RNA的生物发生，精确定位和定量，该核苷酸RNA是将它们用于花药功能的蛋白质及其在植物生育中的作用。先前的工作表明，在轻微的温度压力下，稳健的男性生育能力是必需的：没有它们的花药发育失败。该项目将集中在玉米花药上，因为很容易确定它们所处的发育阶段，它们很容易剖析，有许多发育突变体会影响基因被隔离的花药，并且因为了解男性生育能力对杂化玉米玉米种子的产生至关重要。对花粉发育的分子基础的更详细的了解可以改善种子生产，包括杂交种子；在草丛中，混合玉米和大米显着增强了世界粮食生产。该项目的结果可能包括发现新的遗传途径，以更精确地控制植物中男性生育能力，而生育植物则对环境变化更具弹性。该项目将利用植物基因组学和靶向诱变，尖端成像技术，生物信息学，重点是发育生物学。该项目的更广泛的影响包括通过与长期和成功的本科和高中实习计划进行培训，对植物和计算生物学的学生培训。该项目将专门集中于24-NT分阶段的次级sirnas，称为“ 24-nt Phasirnas”，它们在减数分裂阶段的花药中高度富集。这项工作将在玉米中作为主要物种进行分析，因为存在大量现有的遗传和分子资源。该项目将解决几个重要问题。减数分裂细胞在小RNA及其功能的积累中与体细胞不同吗？哪些转录因子指导设置细胞命运和包括phasirnas的细胞类型特异性特性所需的过渡？如何部署减数分裂阶段来指导花药细胞的命运和分化？ microRNA在何时何地触发前体mRNA以及生物发生或效应蛋白积累？该项目将表征表型和分子遗传分析的功能丧失基因敲除突变，这些突变会影响Phasirnas，包括dicer-like5（DCL5）基因的突变，初步数据证明了男性不育。项目成员将在植物小型RNA生物学和基因组学，细胞生物学和显微镜以及计算方法中进行广泛培训，并通过其个人和小组项目分析小型RNA的本地化，生物发生和生物化学。该奖项反映了NSF的法定任务，并通过使用基础的智力效果和宽阔的范围进行评估，并被认为是值得评估的，并具有值得评估。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Gametophyte genome activation occurs at pollen mitosis I in maize

DOI：
10.1126/science.abl7392
发表时间：
2022-01-28
期刊：
SCIENCE
影响因子：
56.9
作者：
Nelms, Brad;Walbot, Virginia
通讯作者：
Walbot, Virginia

Crowdsourcing biocuration: The Community Assessment of Community Annotation with Ontologies (CACAO).

DOI：
10.1371/journal.pcbi.1009463
发表时间：
2021-10
期刊：
PLoS computational biology
影响因子：
4.3
作者：
Ramsey J;McIntosh B;Renfro D;Aleksander SA;LaBonte S;Ross C;Zweifel AE;Liles N;Farrar S;Gill JJ;Erill I;Ades S;Berardini TZ;Bennett JA;Brady S;Britton R;Carbon S;Caruso SM;Clements D;Dalia R;Defelice M;Doyle EL;Friedberg I;Gurney SMR;Hughes L;Johnson A;Kowalski JM;Li D;Lovering RC;Mans TL;McCarthy F;Moore SD;Murphy R;Paustian TD;Perdue S;Peterson CN;Prüß BM;Saha MS;Sheehy RR;Tansey JT;Temple L;Thorman AW;Trevino S;Vollmer AC;Walbot V;Willey J;Siegele DA;Hu JC
通讯作者：
Hu JC

A cascade of bHLH-regulated pathways programs maize anther development