Regulatory Hierarchies and Roles of Non-Coding RNAs in Maize Anthers

玉米花药中非编码 RNA 的调控层次和作用

基本信息

  • 批准号:
    1649424
  • 负责人:
  • 金额:
    $ 412.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-01-01 至 2020-05-31
  • 项目状态:
    已结题

项目摘要

PI: Blake C. Meyers (University of Delaware)CoPIs: Virginia Walbot (Stanford University) and Greg Abrams (Texas Advanced Computing Center/University of Texas - Austin) Information generated from this project will provide new insights into the regulation of developmental events in the anther, the male reproductive organ, of maize and related grasses - crops that feed much of the world. New insights and resources of broad utility should ultimately contribute to controlling plant fertility in agriculture, particularly in the cereal crops. The project builds and improves on the varied and extensive outreach and education components of the PIs and their contributions via scientific leadership to continue a strong, positive impact on local and scientific communities, and on the next generation of scientists. Implementation of the "Virtual Anther" will make the project data widely accessible while helping students and the public understand how anthers develop. An undergraduate-focused project to sequence and annotate a basal grass genome will provide invaluable data for the project and to all those employing phylogenomics analysis in flowering plants, while training the next generation of plant biologists. All project outcomes will be accessible to the research community through a project website and long-term repositories such as GEO and MaizeGDB. All biological resources such as antibodies, clones and vectors will be available upon request. Seed will be deposited and distributed long-term through the Maize Genetics Cooperative. Software for the "Virtual Anther" will be made open-source and available via the Texas Advanced Computing Center website (https://www.tacc.utexas.edu/tacc-software/).Male sterility is a common phenotype in natural species, functioning as one mechanism to promote outcrossing. Furthermore, control of pollen production by plant breeders is a fundamental technology that continues to underpin hybrid seed production in many crops. In the agricultural context, there is also a growing concern over climate disruption: both heat and cold cause male sterility in crop plants. This study seeks to more broadly understand the coordination of anther development and underlying cellular processes, including chromatin remodeling, as plant cells switch from mitosis to meiosis. The specific goals are to: (1) organize hierarchies of gene expression in individual anther cell types and (2) elucidate functions of small RNAs, particularly two unusual classes of "phased" secondary small RNAs (phasiRNAs) that are highly enriched in grass anthers. These phasiRNAs are processed from an extensive set of coordinately-expressed long non-coding RNA precursors into secondary small RNAs of 21 or 24 nucleotides. Using precisely staged maize anthers and laser microdissected cell types, the project will generate copious transcriptomic and proteomic data, organize transcriptional hierarchies of transcription factors, generate extensive data on small RNAs in a spatiotemporal context, and analyze the function and biogenesis of phasiRNAs. Genetic analysis using existing and engineered mutations will be used to test for essential roles of key transcription and phasiRNA biogenesis factors in anther development. Genetic and "omics" experiments will test new hypotheses about phasiRNA functions in anther cells and in meiosis. Most of the analysis will be conducted in maize because of its exquisite developmental regularity, the large size and ease of anther dissection, and existing molecular and genetic knowledge about maize anthers. Key insights will be evaluated in rice to distinguish general and species-specific points, with additional comparisons to other grasses. To generate new hypotheses, all data will be integrated into a "Virtual Anther" visualization system.
Pi:Blake C.Meyers(特拉华大学)Copis:弗吉尼亚·沃尔伯特(Stanford University)和格雷格·艾布拉姆斯(Greg Abrams)(德克萨斯先进计算中心/德克萨斯大学奥斯汀分校)该项目产生的信息将为调节玉米和相关牧草的雄性生殖器官花药的发育事件提供新的见解。新的见解和广泛实用的资源最终应该有助于控制农业中的植物肥力,特别是在谷类作物中。该项目建立并改进了个人投资促进机构的各种广泛的宣传和教育部分及其通过科学领导作出的贡献,以继续对地方和科学界以及对下一代科学家产生强有力的积极影响。“虚拟花药”的实施将使项目数据得到广泛的获取,同时帮助学生和公众了解花药是如何发育的。一个以本科生为重点的测序和注释基本草类基因组的项目将为该项目和所有在开花植物中进行系统发育分析的人提供宝贵的数据,同时培训下一代植物生物学家。所有项目成果将通过一个项目网站和诸如GEO和MaizeGDB等长期储存库向研究界开放。所有生物资源,如抗体、克隆和载体,将根据要求提供。种子将通过玉米遗传合作社长期储存和分发。用于“虚拟花药”的软件将被开源,并可通过德克萨斯州先进计算中心网站获得。(https://www.tacc.utexas.edu/tacc-software/).Male不育是自然物种中一种常见的表型,它是促进异交的一种机制。此外,植物育种者控制花粉产生是一项基本技术,继续支撑着许多作物的杂交种子生产。在农业方面,人们也越来越担心气候破坏:高温和寒冷都会导致作物雄性不育。这项研究试图更广泛地了解植物细胞从有丝分裂到减数分裂过程中花药发育和潜在细胞过程的协调,包括染色质重塑。具体目标是:(1)组织单个花药细胞类型中基因表达的层次结构;(2)阐明小RNA的功能,特别是高度富含在草药中的两类不寻常的“阶段性”次生小RNA(PhasiRNAs)。这些phasiRNA是从一组广泛的协调表达的长非编码RNA前体加工成21或24个核苷酸的次级小RNA。利用精确分期的玉米花药和激光显微解剖的细胞类型,该项目将产生丰富的转录和蛋白质组数据,组织转录因子的转录层次结构,在时空背景下产生关于小RNA的广泛数据,并分析phasiRNAs的功能和生物发生。使用现有的和工程的突变进行的遗传分析将用于测试关键的转录和phasiRNA生物发生因子在花药发育中的关键作用。遗传学和“组学”实验将检验有关花药细胞和减数分裂中的phasiRNA功能的新假说。大部分的分析将在玉米上进行,因为它精致的发育规律,花药解剖的大小和容易,以及现有的关于玉米花药的分子和遗传知识。将对水稻的关键见解进行评估,以区分一般和物种特定的点,并与其他草本植物进行额外的比较。为了产生新的假设,所有的数据都将被整合到一个“虚拟花药”可视化系统中。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Blake Meyers其他文献

Blake Meyers的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Blake Meyers', 18)}}的其他基金

Roles of meiotic-stage non-coding RNAs in maize anther development
减数分裂阶段非编码RNA在玉米花药发育中的作用
  • 批准号:
    2320971
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
Collaborative Research: RESEARCH-PGR: Extracellular RNA Produced By Plants: What, Where, How, Who, and Why?
合作研究:RESEARCH-PGR:植物产生的细胞外 RNA:什么、在哪里、如何、谁以及为什么?
  • 批准号:
    2141970
  • 财政年份:
    2022
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
Collaborative Research: BBSRC-NSF/BIO: An autonomous registry system for plant microRNAs
合作研究:BBSRC-NSF/BIO:植物 microRNA 自主注册系统
  • 批准号:
    2130883
  • 财政年份:
    2021
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Continuing Grant
EAGER: Single-cell, spatial transcriptomics of plant-fungal interactions using a maskless array technology
EAGER:使用无掩模阵列技术进行植物-真菌相互作用的单细胞空间转录组学
  • 批准号:
    1945854
  • 财政年份:
    2019
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
The Role of Meiotic-Stage Non-Coding RNA in the Modulation of Anther & Pollen Development in Grasses
减数分裂阶段非编码 RNA 在花药调节中的作用
  • 批准号:
    1754097
  • 财政年份:
    2018
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Continuing Grant
ERA-CAPS: Collaborative Research: Role of Extracellular Vesicles in Plant-Microbe Interactions
ERA-CAPS:合作研究:细胞外囊泡在植物-微生物相互作用中的作用
  • 批准号:
    1842698
  • 财政年份:
    2018
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
EDGE: High Efficiency Identification of Products of Homologous Recombination in Plants as a Tool to Test Gene Function
EDGE:高效鉴定植物同源重组产物作为测试基因功能的工具
  • 批准号:
    1827761
  • 财政年份:
    2018
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
IOS: The Function and Evolution of Plant Phased siRNAs in Signaling Pathways and Microbial Interactions
IOS:植物定相 siRNA 在信号通路和微生物相互作用中的功能和进化
  • 批准号:
    1650843
  • 财政年份:
    2016
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Continuing Grant
RCN: An International Arabidopsis Informatics Consortium
RCN:国际拟南芥信息学联盟
  • 批准号:
    1656152
  • 财政年份:
    2016
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Continuing Grant
Regulatory Hierarchies and Roles of Non-Coding RNAs in Maize Anthers
玉米花药中非编码 RNA 的调控层次和作用
  • 批准号:
    1339229
  • 财政年份:
    2014
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Continuing Grant

相似海外基金

Collaborative Research: Constraining the Role of the Antarctic Slope Current on Tracer Exchange at the Antarctic Margin using Model Hierarchies
合作研究:利用模型层次结构约束南极坡流对南极边缘示踪剂交换的作用
  • 批准号:
    2319828
  • 财政年份:
    2024
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
Collaborative Research: Constraining the Role of the Antarctic Slope Current on Tracer Exchange at the Antarctic Margin using Model Hierarchies
合作研究:利用模型层次结构约束南极坡流对南极边缘示踪剂交换的作用
  • 批准号:
    2319829
  • 财政年份:
    2024
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Standard Grant
Elucidation of Characteristics and Functions of Rituals of Japanese New Religions : To Reconsider Their Worldviews and Hierarchies
日本新宗教仪式的特征和功能的阐释:重新考虑他们的世界观和等级制度
  • 批准号:
    22KJ1273
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Cultural Hierarchies in Health: Does inherited sociocultural position (biraderi) shape diet and nutrition among British Pakistani children?
健康中的文化等级:继承的社会文化地位(biraderi)是否影响了英属巴基斯坦儿童的饮食和营养?
  • 批准号:
    ES/X012816/1
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Research Grant
'RITUAL FRACTURE': DISMANTLING PATRIARCHAL HIERARCHIES THROUGH A QUEER, ANTI-CASTE, ECOFEMINIST RITUAL POETICS
“仪式断裂”:通过酷儿、反种姓、生态女性主义的仪式诗学来瓦解父权等级制度
  • 批准号:
    2891647
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Studentship
'RITUAL FRACTURE': DISMANTLING PATRIARCHAL HIERARCHIES THROUGH A QUEER, ANTI-CASTE, ECOFEMINIST RITUAL POETICS
“仪式断裂”:通过酷儿、反种姓、生态女性主义的仪式诗学来瓦解父权等级制度
  • 批准号:
    2904689
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Studentship
CAREER: Programming Heterogeneous Memory Hierarchies
职业:异构内存层次结构编程
  • 批准号:
    2239373
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Continuing Grant
Neural processing of status signals in social hierarchies
社会等级中状态信号的神经处理
  • 批准号:
    10646570
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
Dynamic task scheduling strategies for deep memory hierarchies in the future
未来深度内存层次结构的动态任务调度策略
  • 批准号:
    22KJ0677
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Hierarchies of spatiotemporal anticipation in the human brain
人脑中时空预期的层次结构
  • 批准号:
    10558919
  • 财政年份:
    2023
  • 资助金额:
    $ 412.88万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了