IOS: The Function and Evolution of Plant Phased siRNAs in Signaling Pathways and Microbial Interactions

IOS：植物定相 siRNA 在信号通路和微生物相互作用中的功能和进化

• 批准号: 1650843
• 负责人: Blake Meyers
• 金额: $ 42.27万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-01-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1650843&HistoricalAwards=false
• 关键词: IOS; Function; Evolution; Plant; Phased

The goal of this work is to investigate in plants the function and evolution of a novel class of regulatory small RNAs. Prior work demonstrated that plant genes like those known to confer resistance to pathogens are the targets of a set of "microRNAs"--suppressive regulatory RNAs just 22 nucleotides in length. These microRNAs are believed to function as master regulators of a very large family of protein coding disease-resistance genes, and part of their activity is to trigger the production of additional small RNAs (21 nucleotides in length) that have an unusual, regularly spaced pattern, or "phasing" of biogenesis. The regulation of this gene family, and several others targeted by microRNAs in the same way, is a new paradigm for microRNA function. The investigators have identified these small RNAs as conserved in many crop plants, including soybean, an alfalfa relative, tomato and potato. The aim of this project is to investigate this novel gene regulatory circuit to understand its function in plants, its role in plant defenses or beneficial microbial interactions, and whether it can ultimately be developed as a tool for improving plants or altering plant-microbe interactions to reduce disease or improve beneficial interactions. The project will utilize methods that include plant genomics, plant cell biology, plant-microbial interactions, and RNA analysis. The expected result is the dissection and understanding of a novel and complex gene regulatory mechanism. The broader impacts of the project range from a local impact on the training of students and a post-doc who will be directly involved in the project, to national and international impact to the scientific community in the databases and web tools that the project will release.

这项工作的目标是研究植物中一类新的调节性小RNA的功能和进化。先前的工作表明，植物基因，如那些已知的赋予病原体抗性的基因，是一组“microRNA”的靶点--抑制性调控RNA只有22个核苷酸长。这些microRNA被认为是一个非常大的蛋白质编码抗病基因家族的主要调节因子，它们的部分活性是触发额外的小RNA（长度为21个核苷酸）的产生，这些小RNA具有不寻常的，规则间隔的模式，或生物发生的“阶段”。这个基因家族的调节，以及其他几个以同样方式被microRNA靶向的基因家族，是microRNA功能的新范例。研究人员已经确定这些小RNA在许多作物中是保守的，包括大豆，苜蓿的亲戚，番茄和马铃薯。该项目的目的是研究这种新的基因调控电路，以了解其在植物中的功能，其在植物防御或有益微生物相互作用中的作用，以及它是否最终可以被开发为改善植物或改变植物-微生物相互作用的工具，以减少疾病或改善有益的相互作用。该项目将利用包括植物基因组学，植物细胞生物学，植物-微生物相互作用和RNA分析的方法。预期的结果是解剖和理解一个新的和复杂的基因调控机制。该项目更广泛的影响范围从对学生培训的当地影响和将直接参与该项目的博士后，到该项目将发布的数据库和网络工具对科学界的国家和国际影响。