I-Corps: A point-of-care diagnostic sensor for tuberculosis

I-Corps:结核病即时诊断传感器

基本信息

  • 批准号:
    2323442
  • 负责人:
  • 金额:
    $ 5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-05-01 至 2024-04-30
  • 项目状态:
    已结题

项目摘要

The broader impact/commercial potential of this I-Corps project is the development of point-of-care diagnostic sensor to support health agencies globally in overcoming tuberculosis (TB). Current laminar-flow diagnostic tests do not target the pathogen directly, but instead detect macromolecules produced by the bacteria, mainly lipoarabinomannan (LAM) in urine. However, not all TB patients excrete this molecule into the urine, and LAM concentrations can be affected significantly by the infection sites. This may explain the low sensitivity (~50%) of such assays. TB is the world’s second leading cause of death from a single infectious agent. In 2020, 10 million people were infected, and 1.8 million died. It is recognized that the high mortality is attributed to lack of TB testing affecting 3-6 million people per year, as well as misdiagnoses, late diagnoses, co-infection with HIV and malaria, and lack of adherence to treatment strategy. The proposed technology may overcome the limitations of current tests, especially for developing areas where medical resources are limited.This I-Corps project is based on the development of a point-of-care sensor for bacteria detection in biological fluids. The proposed sensor is built on the interactions of silver (Ag+) and bacteria. It is established that Ag+ can enter various bacterial species through outer membrane proteins, particularly the OmpF—a 39 kD transmembrane protein with a trimeric β-barrel structure, and binds to cytoplasm components, including proteins and nucleic acid. The proposed method is based on detecting the loss of free [Ag+] added to a sample with bacteria, and determining the bacteria number. The detection limit may be as low as 80 bacteria per milliliter sample. Moreover, an open-source potentiometer provides a low-cost and customizable opportunity for such sensor development and translation, especially in under-developed areas with limited budgets. The sensor also allows for wireless data transfer and storage in smartphones, which may allow for large-scale screening and remote healthcare. For TB detection, antibodies that are specific to m. tuberculosis will be integrated in the sensor for selective diagnosis of the presence of the bacteria.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
I-Corps项目的更广泛影响/商业潜力是开发即时诊断传感器,以支持全球卫生机构战胜结核病。目前的层流诊断测试并不直接针对病原体,而是检测细菌产生的大分子,主要是尿液中的脂阿拉伯甘露聚糖(LAM)。然而,并非所有结核病患者都将这种分子排泄到尿液中,并且LAM浓度可能受到感染部位的显著影响。这可以解释这种测定的低灵敏度(~50%)。结核病是世界上第二大单一传染性病原体致死原因。2020年,有1000万人感染,180万人死亡。人们认识到,死亡率高的原因是缺乏结核病检测,每年影响300万至600万人,以及误诊、迟诊、艾滋病毒和疟疾合并感染,以及不坚持治疗战略。拟议的技术可能会克服目前的测试的局限性,特别是对于发展中地区的医疗资源是有限的。这个I-Corps项目是基于一个点的护理传感器的生物流体中的细菌检测的发展。该传感器是建立在银(Ag+)和细菌的相互作用。已经确定Ag+可以通过外膜蛋白,特别是具有三聚体β-桶结构的OmpF-a 39 kD跨膜蛋白进入各种细菌物种,并结合到细胞质组分,包括蛋白质和核酸。 所提出的方法是基于检测添加到具有细菌的样品中的游离[Ag+]的损失,并确定细菌数量。检测极限可低至每毫升样品80个细菌。此外,开源电位计为这种传感器的开发和转换提供了低成本和可定制的机会,特别是在预算有限的欠发达地区。该传感器还允许在智能手机中进行无线数据传输和存储,这可能允许大规模筛查和远程医疗。对于TB检测,特异性针对M.该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Chengpeng Chen其他文献

Simplified One-stop Protocols to Extract Collagen from Various Animal Tissues for 3D Cell Cultures
从各种动物组织中提取胶原蛋白用于 3D 细胞培养的简化一站式方案
Class Agnostic Image Common Object Detection
类无关图像常见对象检测
  • DOI:
    10.1109/tip.2019.2891124
  • 发表时间:
    2019-01
  • 期刊:
  • 影响因子:
    10.6
  • 作者:
    Shuqiang Jiang;Sisi Liang;Chengpeng Chen;Yaohui Zhu;Xiangyang Li
  • 通讯作者:
    Xiangyang Li
Extracellular Matrix Microstructures Modulate Hepatic Methionine Cycle and Methylations
细胞外基质微结构调节肝脏蛋氨酸循环和甲基化
  • DOI:
    10.1021/acs.biomac.4c01748
  • 发表时间:
    2025-06-09
  • 期刊:
  • 影响因子:
    5.400
  • 作者:
    John A. Terrell;Chengpeng Chen
  • 通讯作者:
    Chengpeng Chen
A Low-Cost and Customizable TEER Meter for the Measurements of Cellular Barrier Integrity
用于测量细胞屏障完整性的低成本、可定制的 TEER 测量仪
  • DOI:
    10.26434/chemrxiv.11374260
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Curtis G. Jones;Chengpeng Chen
  • 通讯作者:
    Chengpeng Chen
Emerging 3D printing technologies and methodologies for microfluidic development.
用于微流体开发的新兴 3D 打印技术和方法。
  • DOI:
    10.1039/d2ay00798c
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    3.1
  • 作者:
    G. Kabandana;Tao Zhang;Chengpeng Chen
  • 通讯作者:
    Chengpeng Chen

Chengpeng Chen的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

相似国自然基金

单片三维相变存储器高速高可靠读取技术研究
  • 批准号:
    61904186
  • 批准年份:
    2019
  • 资助金额:
    26.0 万元
  • 项目类别:
    青年科学基金项目
解大型非对称鞍点(Saddle Point) 问题的有效算法的研究
  • 批准号:
    60573157
  • 批准年份:
    2005
  • 资助金额:
    20.0 万元
  • 项目类别:
    面上项目

相似海外基金

I-Corps: Mini-Disc Point-of-Care Personalized Diagnostic Platform
I-Corps:迷你光盘护理点个性化诊断平台
  • 批准号:
    2330092
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Multiplex diagnostic assay using interdigitated nano-sensing technology implemented point-of-care device
I-Corps:使用叉指纳米传感技术实施的护理点设备进行多重诊断测定
  • 批准号:
    2318433
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Artificial Intelligence (AI)-enabled point-of-care diagnostic ultrasound for injured children
I-Corps:为受伤儿童提供人工智能 (AI) 护理点超声诊断
  • 批准号:
    2233863
  • 财政年份:
    2022
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Rapid, Low Cost Point-of-Care Microfluidic Cancer Screening Device
I-Corps:快速、低成本的护理点微流控癌症筛查设备
  • 批准号:
    2224974
  • 财政年份:
    2022
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Development of a rapid point-of-care test for coronavirus (COVID-19) and antibody testing
I-Corps:开发冠状病毒 (COVID-19) 快速护理点测试和抗体测试
  • 批准号:
    2041364
  • 财政年份:
    2020
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Point-of-Care Microfluidic Device for Rapid Pathogen Detection
I-Corps:用于快速病原体检测的护理点微流体装置
  • 批准号:
    1953841
  • 财政年份:
    2020
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Disposable Microflow Cytometer for Point-of-Care and Mobile Testing
I-Corps:用于现场护理和移动测试的一次性微流式细胞仪
  • 批准号:
    1937629
  • 财政年份:
    2019
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Early risk assessment of infant head malformations at the point-of-care
I-Corps:婴儿头部畸形的护理点早期风险评估
  • 批准号:
    1924125
  • 财政年份:
    2019
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: H-meter - point of care device for the analysis of hormones in blood
I-Corps:H-meter - 用于分析血液中激素的护理点设备
  • 批准号:
    1929292
  • 财政年份:
    2019
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
I-Corps: Barcode embedded rapid diagnostic tests for point-of-care fertility tests
I-Corps:嵌入式条形码快速诊断测试,用于即时生育测试
  • 批准号:
    1817594
  • 财政年份:
    2018
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了