权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Revealing neocortical mechanisms for declarative learning: Functional role and cellular mechanisms of primary sensory cortex plasticity for trace eyeblink conditioning in mice.

揭示陈述性学习的新皮质机制：初级感觉皮层可塑性对小鼠微量眨眼调节的功能作用和细胞机制。

基本信息

批准号：
270837099
负责人：
Professor Dr. Cornelius Schwarz
金额：
--
依托单位：
Werner Reichardt-Centrum für integrative Neurowissenschaften (CIN)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/270837099?language=en
关键词：
Revealing neocortical mechanisms declarative learning

项目摘要

Associative learning is characterized by sensible alterations in the behavior of a subject in response to relevant changes in the world. In case the sensory stimulus signaling the changed environment is temporally separate from the to-be-adapted behavior, the association of the two requires mnemonic brain processes. In trace eyeblink conditioning, an established model system for declarative learning, the temporal gap between the CS and the CR is bridged by sustained mPFC neuronal activity. Primary sensory cortices are critical for trace conditioning too, but their exact mechanistic role in bridging the gap is still elusive. Here we propose to study mouse trace eyeblink conditioning, taking advantage of the availability of a tactile variant of the trace eyeblink conditioning paradigm, and the very well-studied primary somatosensory area BCx. We aim at elucidating whether and how BCx represents mnemonic signals and how this is underpinned by re-arrangement of synaptic connections. Our starting point is previous work by others that showed that BCx function is indispensable for this task, and our preliminary work using 2PI that showed that dendritic spines on apical dendrites of L5 pyramids in L1 are eliminated during trace conditioning. In a first set of experiments, mnemonic signals will be searched employing multi-site electrophysiology in BCx and mPFC. Concerted activity in the two structures will be monitored during CS, trace and CR periods. A second set of experiments will focus on the question whether the number of presynaptic excitatory axonal boutons in L1 is changed in concert with the postsynaptic spines using virus-based tracing techniques in combination with 2PI. Finally, using 2PI calcium imaging of apical dendrites during learning we will elucidate whether dendritic tuft calcium transients predict (and possibly cause) spine elimination.

联想学习的特点是受试者在行为上做出明智的改变，以应对世界上的相关变化。在感觉刺激信号改变的环境是暂时分开的适应行为，两者的关联需要记忆的大脑过程。在痕迹眨眼条件反射，一个既定的模型系统的陈述性学习，CS和CR之间的时间差距是桥接持续mPFC神经元活动。初级感觉皮层对痕迹条件反射也很关键，但它们在弥合差距中的确切机制作用仍然难以捉摸。在这里，我们建议研究小鼠痕迹眨眼条件反射，利用触觉的变化的痕迹眨眼条件反射范例的可用性，和非常好的研究初级体感区BCx。我们的目标是阐明是否以及如何BCx代表记忆信号，这是如何支持的重新安排突触连接。我们的出发点是其他人以前的工作，表明BCx功能对于这项任务是不可或缺的，我们使用2PI的初步工作表明，L1中L5金字塔顶端树突上的树突棘在痕迹调节过程中被消除。在第一组实验中，将采用BCx和mPFC中的多位点电生理学来搜索记忆信号。在CS、跟踪和CR期间，将监测两个结构中的协调活动。第二组实验将集中在这个问题上，是否突触前兴奋性轴突终扣在L1的数量变化与突触后棘使用基于病毒的跟踪技术结合2PI。最后，使用2PI钙成像的顶端树突在学习过程中，我们将阐明是否树突簇钙瞬变预测（并可能导致）脊柱消除。