Development of solid state NMR methodology to study RNA and protein-RNA complexes

开发用于研究 RNA 和蛋白质-RNA 复合物的固态 NMR 方法

• 批准号: 274441581
• 负责人: Professorin Dr. Teresa Carlomagno
• 金额: --
• 依托单位: School of Biosciences
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/274441581?language=en
• 关键词: Development; solid; state; NMR; methodology

In this research project we plan to develop novel NMR methodology for structural studies of RNA and RNA-protein complexes by means of solid-state NMR (ssNMR). ssNMR leverages the requirements of moderate size and high solubility that limit structural studies by solution-state NMR, or the necessity of high-resolution diffracting crystals for structure determination by X-ray crystallography. ssNMR has been developing fast in the fields of amyloid fibrils, membrane proteins and large protein complexes; however, significantly fewer studies have been performed on RNA or protein-RNA complexes (RNPs), due to the lack of a general approach to study RNA by ssNMR. Taking advantage of recent development of the NMR hardware, different labeling techniques and modern selective magnetization transfer schemes, we will develop a strategy to determine the structure of RNA by ssNMR. These results will pave the way to study large RNAs and RNPs that cannot be studied either by crystallography or solution-state NMR.

在本研究项目中，我们计划开发新的NMR方法，通过固态NMR（ssNMR）的RNA和RNA-蛋白质复合物的结构研究。ssNMR利用了限制通过溶液状态NMR进行结构研究的中等尺寸和高溶解度的要求，或者利用X射线晶体学进行结构测定的高分辨率衍射晶体的必要性。ssNMR在淀粉样纤维、膜蛋白和大蛋白复合物等研究领域发展迅速，但由于ssNMR研究RNA缺乏通用方法，对RNA或蛋白质-RNA复合物（RNP）的研究相对较少。利用最近发展的核磁共振硬件，不同的标记技术和现代选择性磁化转移计划，我们将开发一种策略，确定RNA的结构ssNMR。这些结果将为研究晶体学或溶液态NMR无法研究的大RNA和RNP铺平道路。