Coordination Funds

协调基金

• 批准号: 283863204
• 负责人: Professor Dr. Dierk Niessing
• 金额: --
• 依托单位: Institut für Pharmazeutische Biotechnologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/283863204?language=en
• 关键词: Coordination; Funds

Work of the last 20 years demonstrated that gene regulation at the level of mRNAs is as important as transcriptional control. In eukaryotic cells, mRNAs are always associated with various RNA-binding proteins in a well-defined manner, forming higher-order complexes. These complexes regulate the temporal and spatial availability of transcripts for translation. They are highly dynamic and change their composition during the life of an mRNA from transcription to degradation. A special type of RNA-based gene regulation is the active transport of mRNAs by molecular motors along the cytoskeleton to the cell periphery, where they are locally translated. This results in subcellular protein gradients. mRNA localization and localized translation is found throughout eukaryotes and plays a role in diverse processes such as asymmetric cell division, embryogenesis, and neuronal plasticity. Impaired mRNA transport factors have been implicated in a number of heritable, mostly neuronal diseases. The aim of the FOR2333 Research Unit is to bring together various experts from throughout Germany, studying different aspects of mRNA localization. The research team consists of scientists from a broad range of expertise, including cell biology, biochemistry, genetics, structural biology and bioinformatics. In our second funding period, we wish to understand (i) how cis-acting elements within localizing mRNAs are defined, (ii) how different domains within one RNA-binding protein cooperate to mediate RNA localization, and (iii) why and how remodeling of mRNA transport complexes occurs when they change their subcellular location or upon environmental cues. We will make use of our strong collaborative ties, established in the first funding period, to train young scientists at regular network meetings, a method workshop and one international conference. In short, this Research Unit brings together junior and senior scientists from throughout Germany to advance our understanding of mRNA localization.

过去20年的研究表明，mRNA水平的基因调控与转录调控同样重要。在真核细胞中，mRNA总是以明确的方式与各种RNA结合蛋白结合，形成更高级的复合物。这些复合体调节转录本在时间和空间上的可用性。它们是高度动态的，并且在mRNA从转录到降解的生命期间改变其组成。一种特殊类型的基于RNA的基因调控是mRNA通过分子马达沿着细胞骨架主动转运到细胞周边，在那里它们被局部翻译。这导致亚细胞蛋白梯度。mRNA定位和定位翻译在真核生物中广泛存在，并在细胞不对称分裂、胚胎发生和神经元可塑性等过程中发挥作用。受损的mRNA转运因子与许多遗传性疾病（主要是神经元疾病）有关。FOR2333研究中心的目标是汇集来自德国各地的专家，研究mRNA定位的不同方面。研究团队由来自细胞生物学、生物化学、遗传学、结构生物学和生物信息学等广泛专业领域的科学家组成。在我们的第二个资助期，我们希望了解（i）如何定义定位mRNA内的顺式作用元件，（ii）一个RNA结合蛋白内的不同结构域如何合作介导RNA定位，以及（iii）当mRNA转运复合物改变其亚细胞位置或环境线索时，它们为什么以及如何发生重塑。我们将利用我们在第一个资助期建立的强大合作关系，在定期网络会议、方法研讨会和一次国际会议上培训年轻科学家。简而言之，这个研究单位汇集了来自整个德国的初级和高级科学家，以促进我们对mRNA定位的理解。