Transport of large cargoes, such as ribosomal subunits, through the permeability barrier of nuclear pore complexes (P08)

通过核孔复合物的渗透性屏障运输大货物，例如核糖体亚基（P08）

• 批准号: 290054213
• 金额: --
• 依托单位: Institut für Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/290054213?language=en
• 关键词: Transport; large; cargoes; such; ribosomal

The nuclear envelope with embedded nuclear pore complexes (NPCs) divides eukaryotic cells into a nuclear and a cytoplasmic compartment. NPCs usually restrict the passage of inert macromolecules. Shuttling nuclear transport receptors (NTRs), however, confer interactions with nucleoporin FG repeats and thereby mediate facilitated NPC-passage of selected cargoes. In this project, we are studying the transport of large (MDa-sized) cargoes using ribosomal subunits as a model system. By generating nanobodies against components of mature ribosomes, fusing them with nuclear transport signals and complexing them with NTRs, we will analyze the number, distribution, and nature of interactions that are required for making mature ribosomal subunits transport-competent. As a complementary approach, we will characterize endogenous, export-competent ribosomal precursors, and study the features needed for their translocation through NPCs and through reconstituted permeability barriers.

具有嵌入核孔复合物（NPCs）的核包膜将真核细胞分为核室和细胞质室。npc通常限制惰性大分子的通过。然而，穿梭的核转运受体（NTRs）与核孔蛋白FG重复序列相互作用，从而介导选择性货物的npc通过。在这个项目中，我们正在使用核糖体亚基作为模型系统研究大型（mda大小）货物的运输。通过生成针对成熟核糖体组分的纳米体，将其与核转运信号融合，并将其与ntr络合，我们将分析使成熟核糖体亚基具有转运能力所需的相互作用的数量、分布和性质。作为一种补充方法，我们将描述内源性、出口能力核糖体前体的特征，并研究它们通过npc和重构的渗透性屏障进行易位所需的特征。