Synthetic genetic analysis, automated microscopy and image analysis (Z03)

对于所有使用酵母作为模式生物的项目（P04 - Schwappach，P06 - Thumm和P13 -Bastling），该技术项目将使用合成基因阵列（SGA）技术提供定制的突变酵母菌株库。这些库将允许各个项目推动他们基于假设的研究计划。系统分析将是CRC的生物学问题的资产，因为我们对隔室门功能的了解受到非常少的模型货物蛋白的广泛使用的限制。在房室接触部位领域，这种方法最近取得了重大进展，并将继续取得进展。中央平台提供了阵列钉扎机器人、液体处理机器人、自动显微镜和图像分析管道的组合，非常适合在酵母中进行系统定位筛选。此外，该平台将支持P07（Kehlenbach）和P10（Brose）在哺乳动物细胞中实施定量成像分析，与手动显微镜和图像分析相比，通量大幅增加。该中心项目使用现有的尖端设备为CRC提供强大的试剂和方法。

For all projects using yeast as a model organism (P04 - Schwappach, P06 - Thumm and P13 - Rehling) this technical project will provide tailor-made libraries of mutant yeast strains using synthetic genetic array (SGA) technology. These libraries will allow the individual projects to drive their hypothesis-based research program. Systematic analysis will be an asset to the biological problems of the CRC because our insight into compartmental gate function is limited by the extensive use of very few model cargo proteins. In the area of compartmental contact sites such approaches have recently spurred major advances and will continue to do so. The central platform provides a combination of array pinning robot, liquid handling robot, automated microscope, and image analysis pipeline ideally suited to perform systematic localization screens in yeast. Furthermore, the platform will support P07 (Kehlenbach) and P10 (Brose) to implement quantitative imaging assays in mammalian cells with substantially increased throughput as compared to manual microscopy and image analysis. This central project uses existing cutting-edge equipment to provide powerful reagents and methodology to the CRC.