Understanding the interaction between enzymes, ionic liquids and water

了解酶、离子液体和水之间的相互作用

• 批准号: 29263522
• 负责人: Professor Dr. Udo Kragl
• 金额: --
• 依托单位: Lehrstuhl für Technische Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/29263522?language=en
• 关键词: Understanding; interaction; between; enzymes; ionic

Biocatalysts, namely enzymes are active in the presence of ionic liquids. However, there is still a lack of understanding how some of the effects observed such as improved substrate solubility or improved enzyme stability can be explained. The goals of this project is described by four topics, which are closely related to each other. The first is the investigation and understanding of the aggregate formation of ionic liquids depending on the type and concentration of solvents or solutes used. The second is to gain insight in the interaction of ionic liquids with proteins in order to understand the requirements to obtain dissolved enzymes active under these conditions. For the complete picture the interaction of ionic liquids with water and enzyme substrates or products has to be understood to explain fully distribution coefficients, mass transport or solubility. Finally the understanding can be used to design a system for an enzymatic gas or solution phase reaction using supported ionic liquids.

生物催化剂，即酶在离子液体存在下具有活性。然而，仍然缺乏理解如何解释所观察到的一些效应，例如改善的底物溶解度或改善的酶稳定性。该项目的目标由四个主题描述，这四个主题彼此密切相关。第一个是调查和了解离子液体的聚集体形成取决于所使用的溶剂或溶质的类型和浓度。第二是深入了解离子液体与蛋白质的相互作用，以了解在这些条件下获得溶解的活性酶的要求。对于完整的图片，离子液体与水和酶底物或产物的相互作用必须被理解，以充分解释分配系数，质量传递或溶解度。最后，该理解可用于设计使用负载离子液体的酶气相或溶液相反应的系统。