Spray encapsulation as novel method for generation of micro encapsulated biocatalysts and their application in multi step synthesis

喷雾封装作为微封装生物催化剂生成的新方法及其在多步合成中的应用

• 批准号: 239209252
• 负责人: Professor Dr. Udo Kragl
• 金额: --
• 依托单位: Lehrstuhl für Technische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/239209252?language=en
• 关键词: Spray; encapsulation; novel; method; generation

Microencapsulation of biocatalysts is versatile and useful method used in biochemical reaction engineering. Within this project spray encapsulation will be investigated for the heterogenisation of enzymes. As a first test system laccases will be investigated. The properties of the resulting enzyme immobilisate will be characterized with respect to surface properties, activity, stability, mass transport and so on. Also the activity against a variety of substrates will be investigated. Besides laccases other enzymes such as Bayer-Villiger-Monooxygenases, hydroxylnitril lyases will be encapsulated as well. The immobilized enzymes will be used in a continuously operated reactor for multi-step-biotransformations. Process control will be achieved using online analytic. Membrane processes and micro-extractors will be used for downstream-processing and in situ removal.

生物催化剂微胶囊化是生化反应工程中一种用途广泛、用途广泛的方法。在这个项目中，将研究喷雾封装以实现酶的异质性。作为第一个测试系统，将对漆酶进行研究。所得到的固定化酶的性质将从表面性质、活性、稳定性、传质等方面进行表征。此外，还将研究其对各种底物的活性。除了漆酶，其他酶，如拜耳-维利格-单加氧酶，羟基硝基裂解酶也将被包裹。固定化酶将在连续运行的反应器中进行多步生物转化。过程控制将使用在线分析来实现。膜过程和微萃取器将用于下游加工和就地去除。

项目成果

Spray congealing as innovative technique for enzyme encapsulation

喷雾凝结作为酶封装的创新技术

• DOI: 10.1002/jctb.5339
• 发表时间: 2018
• 期刊: Journal of Chemical Technology & Biotechnology
• 影响因子: 3.4
• 作者: Engelmann;Kragl U.
• 通讯作者: Kragl U.