Analysis of early events in the Arabidopsis-Verticillium interaction

拟南芥-黄萎病相互作用的早期事件分析

• 批准号: 30286126
• 负责人: Professor Dr. Rudolf Tischner
• 金额: --
• 依托单位: Albrecht-von-Haller-Institut für Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/30286126?language=en
• 关键词: Analysis; early; events; Arabidopsis; Verticillium

Verticilium longisporum is a soil born fungus, which infects Brassicaceae in a compatible interaction and causes significant losses in the yield of several crops. The fungus infects the roots and stays in the xylem causing a wild disease. In the xylem conidia are formed and these spread into the shoot carried by the transpiration stream. However, plugging the xylem is a late event, there are signals produced either by the plant or the fungus that cause earlier incidents. The analyses currently available focus on events more than 2 weeks after inoculation. Advanced flowering, degradation of Rubisco and chlorosis were measured using the pathosystem Arabidopsis/V. dahliae, but the symptoms observed did not correlate with the fungal tissue colonization. This led us to ask, if much earlier events in the infection with the fungus can be found maybe even before the germination of the conidia. Our hypothesis postulates that already the contact between the roots and the conidia is sufficient to initiate responses of the plant tissue. These responses include NO synthesis. We established a sterile pathogen system where a distinct number of conidia (V. longisporum) get in contact with a well-defined number of plants for a controlled period of time. We want to focus on changes in the protein pattern of the soluble fraction and the plasma membrane. We are planning to analyse the responses of Arabidopsis wild type and mutants (with defects in NO-, ethylene-, salicylic acid- and jasmonate ¿ dependent signalling) to the contact with the conidia.

长孢轮枝菌（Verticilium longisporum）是一种土生真菌，以亲和互作的方式侵染油菜科植物，对多种作物造成严重的产量损失。真菌感染根部并停留在木质部，引起野生疾病。在木质部中形成分生孢子，这些分生孢子通过蒸腾流传播到芽中。然而，堵塞木质部是一个晚期事件，有信号产生的植物或真菌，导致早期事件。目前可用的分析重点关注接种后2周以上的事件。提前开花，Rubisco的降解和褪绿使用的病理系统拟南芥/大丽花，但观察到的症状没有相关的真菌组织定殖。 这让我们不禁要问，是否可以发现真菌感染的更早事件，甚至在分生孢子萌发之前。我们的假设假定根和分生孢子之间的接触已经足以引发植物组织的反应。这些反应包括NO合成。我们建立了一个无菌的病原体系统，其中不同数量的分生孢子（长孢伏尔氏菌）与明确定义数量的植物接触一段受控的时间。我们想把重点放在可溶性部分和质膜的蛋白质模式的变化。我们正计划分析拟南芥野生型和突变体（在NO-，乙烯-，水杨酸-和茉莉酸盐依赖的信号转导缺陷）与分生孢子接触的反应。