Axonal local translation and its implications in the pathogenesis of amyotrophic lateral sclerosis

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1) We finished the characterization of our motor neuron cultures from a library of 6 ALS and 4 healthy control human iPSC lines from Cedar Sinai Cell Bank for the occurrence of axonal degeneration, protein aggregation and neuronal cell death.2) We looked at the role of mitochondria and ATP production in correlation with local translation and phase separation for some of the lines.2) We collected cell pellets and culture medium from pre-symptomatic, early onset and late stage of disease motor neurons in culture.3) We started to isolate extracellular vesicles, which will be subjected to proteomics and their content will be tested as biomarkers.4) We started to optimize the proteomic analysis of the collected lysates for cell pellets and media.