Host control of persistent Salmonella infection

持续性沙门氏菌感染的宿主控制

• 批准号: 313128590
• 负责人: Professor Dr. Mathias Walter Hornef
• 金额: --
• 依托单位: Institut für Medizinische Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/313128590?language=en
• 关键词: Host; control; persistent; Salmonella; infection

Chronic infection with Salmonella can develop despite adequate antibiotic treatment. In fact some infected individuals become life-long asymptomatic carriers spreading the infection and maintaining Salmonella infection in the human population. In this research project we will study the immunological mechanisms that support Salmonella persistence. We will employ mixed infections with a collection of isogenic Salmonella strains to identify key sites for the establishment of persistent infection and to define routes of Salmonella dissemination in the host. This approach of mixed infection will be combined with a neonate infection model, a model mimicking relapsing infection after antibiotic treatment and naturally resistant mouse strains. Our preliminary experiments revealed independent sites of persisting Salmonella. We therefore aim to dissect host mechanisms that segregate sites of persistence and to identify differences in myeloid cell subsets to support long term infection. We expect that these experiments will yield exciting insights into the dynamics of chronic Salmonella infection and help to improve the treatment of chronically infected patients.

沙门氏菌的慢性感染可以发展，尽管充分的抗生素治疗。事实上，一些感染者成为终身无症状携带者，在人群中传播感染并维持沙门氏菌感染。在这个研究项目中，我们将研究支持沙门氏菌持久性的免疫机制。我们将采用混合感染与收集的同基因沙门氏菌菌株，以确定建立持续感染的关键网站，并确定沙门氏菌传播的主机。这种混合感染的方法将与新生儿感染模型相结合，该模型模拟抗生素治疗后的复发性感染和天然耐药小鼠品系。我们的初步实验揭示了持续存在沙门氏菌的独立位点。因此，我们的目标是剖析主机的机制，隔离网站的持久性和确定的差异，骨髓细胞亚群，以支持长期感染。我们期望这些实验将对慢性沙门氏菌感染的动力学产生令人兴奋的见解，并有助于改善慢性感染患者的治疗。